亚硫酸钠对人正常二倍体肝细胞的损伤作用程序性坏死相关蛋白受体相互作用蛋白1表达的影响

发布时间：2018-06-20 15:40

本文选题：亚硫酸钠 + 肝损伤　；参考：《环境与健康杂志》2015年12期

【摘要】：目的探讨亚硫酸钠对人正常二倍体肝细胞(HL-7702)的损伤作用及程序性坏死相关蛋白受体相互作用蛋白1(RIP1)表达的影响。方法将处于对数生长期的HL-7702细胞分别暴露于含终浓度为0(对照)、0.01、0.1、1、10 mmol/L亚硫酸钠的培养基中培养2、4、12、24、48 h。采用CCK-8法检测细胞活性,采用全自动生化分析仪检测细胞上清液中丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)以及乳酸脱氢酶(LDH)的活力,采用Western Blot法检测细胞RIP1蛋白的表达水平。结果与对照组相比,1、10 mmol/L亚硫酸钠染毒不同时间的HL-7702细胞存活率降低,差异有统计学意义(P0.05);且随着亚硫酸钠染毒浓度的升高,HL-7702细胞的存活率呈下降趋势。与对照组相比,仅10 mmol/L亚硫酸钠染毒24、48 h HL-7702细胞上清液中的ALT活力较高;1 mmol/L亚硫酸钠染毒4、24 h及10 mmol/L亚硫酸钠染毒2、4 h HL-7702细胞上清液中的AST活力较低,而10 mmol/L亚硫酸钠染毒24、48 h HL-7702细胞上清液中的AST活力较高;10 mmol/L亚硫酸钠染毒2、4 h HL-7702细胞上清液中的LDH活力较低,而染毒24、48 h HL-7702细胞上清液中的LDH活力较高,差异均有统计学意义(P0.05)。与对照组相比,各浓度亚硫酸钠染毒组HL-7702细胞中RIP1蛋白的表达水平均较高,差异多有统计学意义(P0.05)。结论程序性坏死可能参与了亚硫酸钠引起的肝细胞死亡,但其可能机制还有待于进一步研究。
[Abstract]:Objective to investigate the effects of sodium sulfite on the damage of human diploid hepatocytes HL-7702 and the expression of programmed necrosis associated protein receptor interaction protein 1 (RIP1). Methods HL-7702 cells at logarithmic growth stage were exposed to a culture medium containing 0 (0. 01) (control 0. 01) and 10 mmol 路L ~ (-1) sodium sulfite, respectively. The activity of cells was detected by CCK-8 method, and the activities of alanine aminotransferase (alt), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) in supernatant of cells were detected by automatic biochemical analyzer. The expression of RIP1 protein was detected by Western blot. Results compared with the control group, the survival rate of HL-7702 cells exposed to 10 mmol / L sodium sulfite decreased significantly (P 0.05), and the survival rate of HL-7702 cells decreased with the increase of sodium sulfite concentration. Compared with the control group, the alt activity in the supernatant of HL-7702 cells exposed to 10 mmol / L sodium sulfite for 24 h or 48 h was higher than that in the supernatant of HL-7702 cells exposed to 10 mmol / L sodium sulfite for 24 h and 10 mmol / L sodium sulfite for 24 h and 2 h, respectively, and the AST activity in the supernatant of HL-7702 cells was lower than that in the supernatant of HL-7702 cells. The activity of AST in the supernatant of HL-7702 cells was higher than that in the supernatant of HL-7702 cells exposed to 10 mmol / L sodium sulfite for 24 h for 48 h. The activity of LDH in the supernatant of HL-7702 cells was lower than that in the supernatant of HL-7702 cells treated with 10 mmol / L sodium sulfite for 2 h or 4 h, but the activity of LDH in the supernatant of HL-7702 cells was higher than that in the supernatant of HL-7702 cells at 24 h after exposure to 24 mmol / L sodium sulfite. The difference was significant (P 0.05). Compared with the control group, the expression of RIP1 protein in HL-7702 cells exposed to sodium sulfite at different concentrations was higher than that in the control group, and the difference was statistically significant (P 0.05). Conclusion programmed necrosis may be involved in the hepatic cell death induced by sodium sulfite, but the possible mechanism remains to be further studied.
【作者单位】：山西医科大学公共卫生学院环境卫生学教研室;
【基金】：山西省回国留学人员科研资助项目(2014-034) 山西省留学回国人员科技活动择优资助项目(晋人社厅函[2014]376号)
【分类号】：R114

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