甲基汞诱导大鼠皮层星形胶质细胞凋亡活性分析

发布时间：2018-06-21 00:04

  本文选题：甲基汞 + 星形胶质细胞　； 参考：《药物分析杂志》2015年07期

【摘要】：目的:研究氯化甲基汞(MMC)对原代培养的大鼠皮质星形胶质细胞存活和凋亡的影响。方法:MMC 0.01、0.05、0.1、0.5、1、2、4和8μmol·L-1孵育原代培养大鼠皮质星形胶质细胞3~4 h,MTT检测星形胶质细胞活性,Hoechst 33342荧光染色和流式细胞仪测定细胞的凋亡,JC-1荧光探针检测线粒体膜电位,Western-blotting法测定凋亡诱导因子和细胞色素C的释放。结果:MMC浓度≤0.1μmol·L-1作用0~6 h,星形胶质细胞的活力无显著变化;暴露时间12~24 h,细胞活力显著降低(P0.05)。MMC浓度≥0.5μmol·L-1,星形胶质细胞活力呈浓度依赖性和时间依赖性降低(r浓度=0.952~0.987,P0.05;r时间=0.831~0.976,P0.05);MMC浓度≤0.1μmol·L-1,随着时间延长使星形胶质细胞凋亡率增加(P0.05)。MMC浓度≥0.5μmol·L-1作用12 h,凋亡率最高,12 h后凋亡率降低,坏死率增高(P0.05);MMC 0.5μmol·L-1呈时间依赖性降低线粒体膜电位(r=0.988,P0.05),凋亡诱导因子和细胞色素C的释放增加。结论:氯化甲基汞可通过线粒体凋亡途径诱导星形胶质细胞凋亡。
[Abstract]:Aim: to study the effects of methylmercury chloride (MMC) on the survival and apoptosis of primary cultured rat cortical astrocytes. Methods the primary cultured rat cortical astrocytes were incubated with 0. 01 0. 05 and 0. 1 0. 5 mol 路L ~ (-1) and 8 渭 mol 路L ~ (-1) for 3 ~ 4 h. The activity of astrocytes was detected by Hoechst 33342 fluorescence staining and the apoptosis was detected by flow cytometry. The mitochondrial membrane potential was detected by Western-blotting with JC-1 fluorescence probe. Release of apoptosis-inducing factor and cytochrome C. Results the activity of astrocytes did not change significantly when the concentration of 0 渭 mol L-1 was less than 0.1 渭 mol L-1 for 6 h. After exposure for 12h, the cell viability decreased significantly (P 0.05N 路MMC concentration 鈮，

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