甜菜碱对蛋氨酸诱导大鼠高同型半胱氨酸血症的抑制作用

发布时间：2018-06-25 15:10

本文选题：甜菜碱 + 蛋氨酸　；参考：《营养学报》2016年02期

【摘要】：目的探讨不同水平蛋白下补充甜菜碱对蛋氨酸诱导大鼠高同型半胱氨酸血症的抑制效果的影响。方法将48只健康Wistar大鼠随机分为低蛋白组:10%酪蛋白(10C),10C+0.75%L-Methionine(10CM),10CM+1.0%Betaine和高蛋白组:40%酪蛋白(40C),40C+0.75%L-Methionine(40CM),40CM+1.0%Betaine。实验喂养14 d后处死动物,采集血液、肝脏样品,用于测定血液生化指标及肝脏蛋氨酸中间代谢物和酶学指标。结果甜菜碱的添加显著抑制了低蛋白膳食中蛋氨酸诱导的同型半胱氨酸浓度的升高,部分抑制了高蛋白膳食中蛋氨酸诱导Hcy浓度的升高,同时使肝脏SAM浓度和SAH比值升高。甜菜碱的添加还有效地恢复了由Met添加诱导的Bet浓度的降低,显著升高了BHMT活性。结论甜菜碱通过促进Hcy的再甲基化,降低了肝脏和血液中Hcy的浓度,显著地抑制了由Met添加诱导的高同型半胱氨酸血症的发生。
[Abstract]:Objective to investigate the inhibitory effect of betaine supplementation at different levels on methionine induced hyperhomocysteinemia in rats. Methods 48 healthy Wistar rats were randomly divided into 10% 10% casein (10C) 10C 0.75 L-Methionine (10CM) 1.0taine and 40C 0.75 L-Methionine (40CM) 1.0taine in high protein group. After 14 days of feeding, the animals were killed, blood and liver samples were collected, and blood biochemical indexes, liver methionine intermediate metabolites and enzymatic indexes were determined. Results the addition of betaine significantly inhibited the increase of homocysteine concentration induced by methionine in low protein diet, partially inhibited the increase of Hcy concentration induced by methionine in high protein diet, and increased the SAM concentration and SAH ratio of liver. The addition of betaine also effectively restored the decrease of Bet concentration induced by Met addition and significantly increased the activity of BHMT. Conclusion Betaine can decrease the concentration of Hcy in liver and blood by promoting the remethylation of Hcy and inhibit the occurrence of hyperhomocysteinemia induced by Met significantly.
【作者单位】：中国疾病预防控制中心营养与健康所;
【基金】：中国营养学会营养科研基金-帝斯曼专项科研基金(No.2014-059)
【分类号】：R151

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