贝类腹泻性毒素现场高通量前处理及快速检测仪器设计

发布时间：2018-06-27 13:03

本文选题：贝类腹泻性毒素 + 前处理装置　；参考：《传感技术学报》2017年01期

【摘要】：为实现贝类腹泻性毒素的现场快速检测,本论文设计了一种现场高通量前处理装置及基于移动终端的快速检测系统。通过使用酶联免疫反应技术对腹泻性贝毒进行显色反应,结合基于移动终端的快速检测装置,采集显色反应结果图像,实现准确快速的检测结果分析。采用所设计的前处理装置与实验室手工前处理标准方法对比,其回收率分别为89%和93%,结果表明,本文设计的前处理装置能够满足现场检测分析的需求。通过加标样品检测,采用移动终端的快速检测系统测量标准差为0.13,平均回收率89.5%。实验结果表明,该检测系统的准确性和重复性满足实际检测的需求。最后,针对实际样品,与酶标仪检测结果相比较,表明了所设计的现场高通量前处理装置及快速检测系统能达到对贝类腹泻性毒素现场快速精确检测,为贝类腹泻性毒素现场检测提供了新的方法和仪器。
[Abstract]:In order to detect shellfish diarrhea toxin quickly, a high throughput preprocessing device and a rapid detection system based on mobile terminal are designed in this paper. By using enzyme linked immunosorbent assay (Elisa), the color reaction of diarrhoeal shellfish was carried out, and the rapid detection device based on mobile terminal was used to collect the image of the result of color reaction, and to realize the accurate and rapid analysis of the result of detection. By comparing the designed preprocessing device with the manual pretreatment standard method, the recoveries are 89% and 93%, respectively. The results show that the preprocessing device designed in this paper can meet the requirements of field detection and analysis. By standard sample detection, the standard deviation of mobile terminal is 0.13, and the average recovery is 89.5%. The experimental results show that the accuracy and repeatability of the detection system meet the needs of the actual detection. Finally, compared with the results of enzymatic marker, the designed high throughput pretreatment device and rapid detection system can be used to detect shellfish diarrhoeal toxins quickly and accurately. It provides a new method and instrument for the field detection of shellfish diarrhoeal toxin.
【作者单位】：浙江大学生物传感器国家专业实验室生物医学工程教育部重点实验室生仪学院;
【基金】：国家海洋公益专项项目(201305010)
【分类号】：R155.3;TP274

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