桶装水中铜绿假单胞菌耐药性分析及RAPD-PCR分型研究

发布时间：2018-06-28 22:51

本文选题：铜绿假单胞菌 + 耐药性　；参考：《现代食品科技》2017年04期

【摘要】：采用Kirby-Bauer纸片扩散法和随机扩增多态性DNA(Random Amplified Polymorphic,RAPD)分型法对23株水源性铜绿假单胞菌进行耐药性及遗传多样性研究。药敏实验结果显示,23株分离株对磺胺甲基异恶唑/甲氧苄氨嘧啶(SXT)、四环素(TE)、米诺环素(MH)的耐药率分别为69.4%、13.2%和39.2%,对另外13种抗生素的敏感性几乎100%。RAPD-PCR指纹图谱聚类分析显示,在相对系数为62%时,引物208将24株菌分为5簇(A~E),其中C为主要的簇,引物272将24株菌分为4簇(F~I),其中I和G簇为主要的簇。23株分离株中无耐药菌株主要集中在B簇,3株多重耐药菌株集中在D簇,对SXT、MH耐药菌株主要集中在I簇。本研究发现水源性铜绿假单胞菌具有较高的遗传多样性,且存在多重耐药,为水源性铜绿假单胞菌的污染溯源和控制提供了相应的数据支持。
[Abstract]:The resistance and genetic diversity of 23 strains of waterborne Pseudomonas aeruginosa were studied by Kirby-Bauer disk diffusion and random Amplified polymorphic DNA (RAPD) typing. The results of drug sensitivity test showed that the resistance rates of 23 isolates to sulfamethoxazole / trimethoprim (SXT), tetracycline (te) and minocycline (MH) were 69.442% and 39.2%, respectively. The sensitivity to the other 13 antibiotics was almost 100% by RAPD-PCR. When the relative coefficient was 62, primer 208 divided 24 strains into 5 clusters (Agne), in which C was the main cluster. Primer 272 divided 24 strains into 4 clusters (FN I), in which I and G clusters were the main cluster. The non-resistant strains were mainly concentrated in cluster B, 3 multidrug resistant strains were concentrated in D cluster, and the resistant strains to SXTMH were mainly concentrated in cluster I. In this study, it was found that Pseudomonas aeruginosa had high genetic diversity and multidrug resistance, which provided data support for the trace and control of the contamination of Pseudomonas aeruginosa in water source.
【作者单位】：广东省食品检验所广东省酒类检测中心;
【基金】：国家自然基金项目(31301478)
【分类号】：R123

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