出生前暴露1800MHz和WiFi叠加射频场对大鼠行为发育及认知的影响

发布时间：2018-06-30 00:00

本文选题：出生前暴露 + 1800MHz电磁波　；参考：《昆明医科大学》2017年硕士论文

【摘要】：[目的]探测1800MHz电磁波和1800MHz与WiFi叠加射频场暴露对仔代行为发育及认知的影响。[方法]健康7周龄SPF级Wistar大鼠36只,雌鼠24只,雄鼠12只,适应环境一周后,雌雄鼠2:1合笼交配后将孕鼠随机分为两个实验组和两个对照组,每组6只。实验一组接受1800MHz电磁波(功率密度为1mW/cm2)暴露,实验二组接受1800MHz和WiFi叠加射频场(功率密度为1mW/cm2)暴露,暴露时间从孕0天至孕20天于每晚20:00～次日清晨8:00,各实验组分别设置虚拟暴露作为对照。暴露期间动物自由饮水和饮食,且环境背景(温度、湿度、背景噪声、通风、光照等)条件稳定。暴露完成后,待孕鼠产下仔鼠,观查并测量生理发育和行为发育指标,待仔鼠长至三周龄和七周龄测水迷宫后取海马组织用高效液相色谱仪检测四种氨基酸类神经递质(Glu、Gly、GABA、Asp)含量。[结果]1.发育指标:(1)生理发育指标:体重、睁眼时间两实验组与对照组比较有差异(p0.05),与对照组比较体重存在下降趋势,睁眼时间延长;其他生理发育指标均无统计学差异。(2)行为发育指标:前肢悬挂达标日龄两实验组与对照组比较有差异(p0.05),与对照组比较达标日龄降低,其他行为发育指标均无统计学差异。2.水迷宫实验:(1)三周龄仔鼠水迷宫实验:1800MHz电磁波暴露下,可视平台实验,雄性仔鼠实验第二天实验组较对照组逃避潜伏期延长(p0.05),雌性仔鼠实验第一天和第二天实验组较对照组逃避潜伏期延长(p0.05);隐匿平台实验,雄性仔鼠实验第一天至第四天实验组较对照组逃避潜伏期延长(p0.05),雌性仔鼠实验第一天至第三天实验组较对照组逃避潜伏期延长(p0.05)。1800MHz与WiFi叠加射频场暴露下,可视平台实验,雌性仔鼠实验第一天实验组较对照组避潜伏期延长(p0.05);隐匿平台实验,雄性和雌性仔鼠实验第一天至第四天实验组均较对照组逃避潜伏期延长(p0.05)。(2)七周龄仔鼠水迷宫实验:1800MHz电磁波暴露下,可视平台实验,雄性仔鼠实验第二天实验组较对照组逃避潜伏期延长(p0.05);隐匿平台实验,雄性仔鼠实验第二天与第三天实验组较对照组逃避潜伏期延长(p0.05),雌性仔鼠实验第一天实验组较对照组逃避潜伏期缩短(p0.05);空间探索实验,雌性仔鼠穿台次数实验组较对照组多(p0.05),平台象限时间百分比(%)较对照组高(p0.05)。1800MHz与WiFi叠加射频场暴露下,隐匿平台实验,雄性仔鼠实验第一天、第三天和第四天实验组均较对照组逃避潜伏期缩短(p0.05),雌性仔鼠实验第四天实验组较对照组逃避潜伏期缩短(p0.05);空间探索实验,雄性仔鼠平台象限时间百分比(%)实验组较对照组高(p0.05)。3.四种氨基酸类神经递质测定:1800MHz电磁波暴露下,实验组三周龄仔鼠海马组织Asp表达量与对照组比较表达上调(p0.05)。1800MHz与WiFi叠加射频场暴露下,实验组三周龄仔鼠海马组织Asp表达量与对照组比较表达上调(p0.05),七周龄仔鼠海马组织Glu表达量与对照比较表达下调(p0.05);三周龄与七周龄比较,实验组三周龄仔鼠海马组织Asp表达量较七周龄高,(p0.05)。[结论]本实验条件下:1. 1800MHz电磁波和1800MHz与WiFi叠加射频场暴露对仔鼠体重和睁眼时间有影响;对前肢悬挂影响还需进一步验证。2. 1800MHz电磁波和1800MHz与WiFi叠加射频场暴露对三周龄仔鼠空间学习能力有影响,未发现对三周龄仔鼠空间记忆能力的影响;对七周龄仔鼠空间学习与记忆能力影响还需进一步验证。3. 1800MHz电磁波和1800MHz与WiFi叠加射频场暴露使三周龄仔鼠海马组织氨基酸类神经递质Asp表达上调;后者可使七周龄仔鼠Glu表达下调。4.尚不明确WiFi对仔鼠学习记忆的影响。
[Abstract]:[Objective] to detect the effects of 1800MHz electromagnetic wave and 1800MHz and WiFi superposition radio frequency field exposure on the behavior development and cognition of the offspring. [Methods] 36 healthy 7 week old Wistar rats, 24 female rats and 12 male rats, after one week of adaptation to the environment, the female and male rats were randomly divided into two experimental groups and two control groups after 2:1 cage mating, and 6 rats in each group were tested. One group was exposed to 1800MHz electromagnetic wave (power density 1mW/cm2). The two groups were exposed to 1800MHz and WiFi superposition radiofrequency field (power density 1mW/cm2). The exposure time from 0 days of pregnancy to 20 days of pregnancy was at 20:00 every night to 8:00 in the morning, and the experimental groups set up virtual exposure as control. The background (temperature, humidity, background noise, ventilation, light and so on) was stable. After the exposure was completed, the offspring rats were produced, and the physiological development and behavioral development indexes were observed and measured. The hippocampus tissue was measured by high performance liquid chromatograph (Glu, Gly, GABA, Asp) by high performance liquid chromatograph after three weeks of age and seven water maze. [results]1. development indicators: (1) physiological development indicators: body weight, eye opening time two experimental group and control group is different (P0.05), compared with the control group, the body weight has a downward trend, the eye opening time is prolonged, and other physiological development indexes are not statistically different. (2) the behavioral development index: the two experimental group of the forelimb suspension was compared with the control group. Compared with the control group (P0.05), compared with the control group, the age of the standard days was lower, and the other behavioral development indexes were not statistically different in the.2. water maze experiment: (1) the water maze test of the three week old rats: the 1800MHz electromagnetic wave exposure, the visual platform experiment, the second day experiment group of the male offspring rats prolonged the escape latency (P0.05) and the female offspring experiment. In one day and second days, the escape latency was prolonged (P0.05) compared with the control group, and the escape latency of the experimental group was prolonged (P0.05) in the first day to the fourth day of the male rat experiment, and the experimental group of the female offspring was longer than the control group in the first day to the third day (P0.05).1800MHz and WiFi superimposed radio frequency field storm. Exposure, visual platform experiment, the first day of the female experiment group was longer than the control group (P0.05), the experiment group was concealed, the experiment group of the male and the female rat were all longer than the control group (P0.05) for the first day to fourth days. (2) the water maze experiment of the seven week old rat: under the exposure of 1800MHz electromagnetic wave, the visual platform experiment, In the second day experiment group, the escape latency of the experimental group was longer than that of the control group (P0.05); the hiding platform experiment, the experiment group of the male offspring and the third day experiment group were longer than the control group, the escape latency was prolonged (P0.05). The first day experiment group of the female offspring was shorter than the control group (P0.05); the space exploration experiment, the female offspring were wearing the platform. The experimental group was more than the control group (P0.05), the percentage of the platform quadrant time (%) was higher than the control group (P0.05).1800MHz and WiFi superposition radiofrequency field exposure, concealed platform experiment, the first day of the male offspring experiment, the third day and the fourth day experiment group were shorter than the control group (P0.05), and the fourth day experiment group of the female offspring rats was compared with the control group. The escape latency shortened (P0.05); space exploration experiment, the percentage (%) of the male offspring's platform quadrant time (%) was higher than the control group (P0.05).3. four amino acid neurotransmitters: under the 1800MHz electromagnetic wave exposure, the expression of Asp expression in the hippocampus of the three week old rats in the experimental group was up to up up (P0.05).1800MHz and WiFi Under field exposure, the expression of Asp expression in hippocampus of three week old rats was up regulated compared with the control group (P0.05). The expression of Glu expression in hippocampal tissue of seven week old rats was down regulated compared with the control group (P0.05). The Asp expression of hippocampus tissue in the experimental group was higher than that at the age of seven weeks at the age of three weeks and at the age of seven weeks, (P0.05). [Conclusion] the experimental conditions were under the condition of this experiment. 1. 1800MHz electromagnetic wave and 1800MHz and WiFi superposition radiofrequency field exposure have influence on the weight and eye opening time of the offspring, and the effects on the forelimb suspension need to be further verified by the influence of the.2. 1800MHz electromagnetic wave and the radio frequency field of 1800MHz and WiFi on the spatial learning ability of the three week old rats, and no effect on the spatial memory ability of the offspring of the three week old mice. The influence of.3. 1800MHz electromagnetic wave and 1800MHz and WiFi superposition radiofrequency field on the expression of amino acid neurotransmitter Asp in the hippocampus of three week old rats was further verified by the influence of the.3. 1800MHz electromagnetic wave and the superposition of the radiofrequency field in the hippocampus of the offspring. The latter could reduce the effect of WiFi on the learning and memory of the offspring of the offspring of the offspring of seven week old mice.
【学位授予单位】：昆明医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R14

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