母鼠金雀异黄素干预对雄性子代大鼠肥胖的影响

发布时间：2018-07-10 08:31

本文选题：金雀异黄素 + 子代　；参考：《环境与职业医学》2017年05期

【摘要】：[目的]研究母鼠金雀异黄素干预对雄性子代大鼠肥胖的影响。[方法]选用性成熟雌性大鼠,受孕后在孕期及哺乳期间均给予不同剂量的金雀异黄素干预,并设立对照组。干预组母鼠采用添加金雀异黄素的饲料喂饲,剂量分别为3 600、1 200、400 mg/kg。子鼠断乳后采用未添加金雀异黄素的饲料喂饲,直至8周末。实验期间每周观察雄性子鼠的体重;实验结束后测定大鼠身长计算Lee's指数,留取脂肪组织,计算脂肪脏器系数,分离血清进行血脂、血糖、胰岛素和炎性因子的检测。[结果]实验8周末,与对照组相比:3 600 mg/kg组子鼠体重降低(P=0.028)、高密度脂蛋白升高(P=0.038),3 600 mg/kg组和1 200 mg/kg子鼠组Lee's指数、总脂肪湿重、血糖水平降低(P=0.031,P=0.049;P=0.009,P=0.016;P=0.033,P=0.026)。实验组子鼠胰岛素、炎性因子未发生明显变化。[结论]孕期及哺乳期金雀异黄素干预,可以降低雄性子鼠肥胖的发生。
[Abstract]:Objective: to study the effect of genistein on obesity in male offspring rats. [methods] sexual mature female rats were treated with different doses of genistein during pregnancy and lactation, and the control group was set up. In the intervention group, the female rats were fed with a diet supplemented with genistein at a dose of 1 200400 mg / kg / kg, respectively. After weaning, the offspring were fed with the feed without genistein until the end of 8 weeks. At the end of the experiment, the body length of rats was measured to calculate the Leehs index, to retain adipose tissue, to calculate the fat organ coefficient, to separate the serum for the detection of blood lipids, blood glucose, insulin and inflammatory factors. [results] at the end of the 8th week of the experiment, compared with the control group, the weight loss (P0. 028), high density lipoprotein (HDL-C) elevated (P0. 038), Lees index, total fat wet weight, and blood glucose level of the mice in the w / w 3 600 mg/kg group were lower than those in the control group (P 0. 031, P 0. 049, P 0. 009, P 0. 016, P 0. 033, P 0. 026). There was no significant change in insulin and inflammatory factors in the experimental group. [conclusion] the intervention of genistein during pregnancy and lactation can reduce the incidence of obesity in male offspring.
【作者单位】：哈尔滨医科大学公共卫生学院环境卫生学教研室;
【基金】：国家自然科学基金项目(编号:81202190) 黑龙江省卫生厅课题(编号:2012-768) 哈尔滨医科大学创新科学研究基金项目(编号:2016JCZX21)
【分类号】：R114

【参考文献】