尿石素A对巨噬细胞极化及巨噬-泡沫细胞形成的作用

发布时间：2018-07-12 20:03

本文选题：尿石素A + 巨噬细胞极化　；参考：《食品科学》2017年13期

【摘要】：旨在探究尿石素A对巨噬细胞极化及对巨噬-泡沫细胞形成的影响及相关的分子机制。结果发现,尿石素A通过调控不同标志基因的表达,不仅能够抑制RAW264.7小鼠巨噬细胞向促炎的M1型巨噬细胞极化,还能促进自然状态巨噬细胞向M2型巨噬细胞极化;油红O染色发现尿石素A能够显著抑制巨噬-泡沫细胞形成,实时荧光定量聚合酶链式反应检测说明尿石素A能够抑制胆固醇合成基因羟甲基戊二酸单酰辅酶A还原酶和脂肪酸合成酶基因的转录;此外,尿石素A显著上调三磷酸腺苷结合盒转运蛋白A1和G1基因的表达,该两个基因表达与促进了胆固醇的排出相关。本研究证明了尿石素A对巨噬细胞极化具有调控作用,同时尿石素A能够抑制巨噬-泡沫细胞形成和胆固醇合成。这些结果揭示了尿石素A具有潜在的抗动脉粥样硬化的作用,为之后深入研究提供了理论参考。
[Abstract]:The aim of this study was to investigate the effects of urolitol A on the polarization of macrophages and the formation of macrophage-foam cells and the related molecular mechanisms. The results showed that urolitol A could not only inhibit the polarization of macrophages from RAW264.7 mice to type M1 macrophages, but also promote the polarization of natural macrophages to M2 type macrophages by regulating the expression of different marker genes. Oil red O staining showed that urostone A could significantly inhibit the formation of macrophages and foam cells. Real-time fluorescence quantitative polymerase chain reaction showed that urostone A could inhibit the transcription of the cholesterol synthase gene hydroxymethyl glutaryl coA reductase and fatty acid synthase gene. The expression of adenosine triphosphate binding cassette transporter A1 and G1 genes was significantly up-regulated by urolithiasis A, which was related to the promotion of cholesterol excretion. This study demonstrated that urolitol A can regulate the polarization of macrophages, and urolide A can inhibit the formation of macrophage foam cells and cholesterol synthesis. These results suggest that urolitol A has a potential anti-atherosclerosis effect, which provides a theoretical reference for further research.
【作者单位】：西北农林科技大学食品科学与工程学院;中国农业大学食品科学与营养工程学院北京食品营养与人类健康高精尖创新中心;定兴县医院;西安交通大学生命科学与技术学院;
【基金】：教育部“新世纪优秀人才支持计划”项目(NCET-13-0488) “十二五”国家科技支撑计划项目(2015BAD16B08) 北京市食品营养与人类健康高精尖创新中心项目(201502910110942) 中央高校基本科研业务费专项资金项目(2452017146)
【分类号】：R151

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