维生素E拮抗双酚A对卵巢颗粒细胞性激素分泌的抑制作用
发布时间：2018-07-15 07:59
【摘要】：目的·研究维生素E能否对双酚A(BPA)的雌性性激素干扰效应起拮抗作用,探索拮抗作用的适宜浓度。方法·提取大鼠卵巢原代颗粒细胞。不同浓度BPA(0、0.01、0.1、1、10、100μmol/L)孵育48 h后,收集并用ELISA法测定细胞培养基中的雌二醇(E_2)及孕酮(P_4)浓度。采用健康女性卵泡液中的平均浓度(简称"人体平均浓度",5μmol/L)或高浓度(25μmol/L)维生素E(α-生育酚)单独孵育或与BPA(10、100μmol/L)共同孵育颗粒细胞48 h后,测定培养液中性激素浓度。结果·10μmol/L及100μmol/L BPA可使大鼠原代卵巢颗粒细胞分泌性激素水平呈浓度依赖性降低(P0.05)。25μmol/L维生素E单独孵育时可分别使E_2和P_4浓度显著升高(44.89±15.18)%和(43.33±8.82)%(P0.05);维生素E(5μmol/L或25μmol/L)和BPA共同孵育时,性激素浓度恢复正常,与阴性对照组相比,差异无统计学意义(P0.05)。结论·人体平均浓度及高浓度维生素E均可拮抗BPA导致的性激素降低效应,而高浓度维生素E的改善效果相对显著。
[Abstract]:Objective to study whether vitamin E can antagonize the female sex hormone interference effect of bisphenol A (BPA) and explore the appropriate concentration of the antagonism. Methods the primary granulosa cells of rat ovary were extracted. The concentrations of estradiol (ESP) and progesterone (PST4) in cell culture medium were determined by Elisa after incubated with different concentrations of BPA (0. 01 ~ 0. 1 ~ 0. 1 ~ 1 ~ (-1) 10 100 渭 mol 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) for 48 h. The average concentration of vitamin E (伪 -tocopherol) in healthy female follicular fluid (5 渭 mol / L for short) or high concentration (25 渭 mol / L) of vitamin E (伪 -tocopherol) was incubated alone or co-incubated with 10100 渭 mol / L of BPA for 48 h. The concentration of sex hormone in the medium was measured. Results 10 渭 mol / L and 100 渭 mol / L BPA decreased the level of sex hormones secreted by primary ovarian granulosa cells in a concentration-dependent manner (P0.05) .25 渭 mol / L vitamin E increased significantly (44.89 卤15.18)% and (43.33 卤8.82)% (P0.05), respectively, and vitamin E (5 渭 mol / L or 25 渭 mol / L) and BPA were incubated with BPA, respectively. Sex hormone concentration returned to normal, compared with the negative control group, the difference was not statistically significant (P0.05). Conclusion the average concentration of vitamin E and high concentration of vitamin E can antagonize the decrease of sex hormone induced by BPA, but the improvement of high concentration of vitamin E is relatively significant.
【作者单位】： 上海交通大学医学院附属新华医院环境与儿童健康教育部和上海市重点实验室;
【基金】：国家重点基础研究发展计划(“973”计划)(2014CB943300) 上海市教育委员会高峰高原学科建设计划(20152220)~~
【分类号】：R114
[Abstract]:Objective to study whether vitamin E can antagonize the female sex hormone interference effect of bisphenol A (BPA) and explore the appropriate concentration of the antagonism. Methods the primary granulosa cells of rat ovary were extracted. The concentrations of estradiol (ESP) and progesterone (PST4) in cell culture medium were determined by Elisa after incubated with different concentrations of BPA (0. 01 ~ 0. 1 ~ 0. 1 ~ 1 ~ (-1) 10 100 渭 mol 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) for 48 h. The average concentration of vitamin E (伪 -tocopherol) in healthy female follicular fluid (5 渭 mol / L for short) or high concentration (25 渭 mol / L) of vitamin E (伪 -tocopherol) was incubated alone or co-incubated with 10100 渭 mol / L of BPA for 48 h. The concentration of sex hormone in the medium was measured. Results 10 渭 mol / L and 100 渭 mol / L BPA decreased the level of sex hormones secreted by primary ovarian granulosa cells in a concentration-dependent manner (P0.05) .25 渭 mol / L vitamin E increased significantly (44.89 卤15.18)% and (43.33 卤8.82)% (P0.05), respectively, and vitamin E (5 渭 mol / L or 25 渭 mol / L) and BPA were incubated with BPA, respectively. Sex hormone concentration returned to normal, compared with the negative control group, the difference was not statistically significant (P0.05). Conclusion the average concentration of vitamin E and high concentration of vitamin E can antagonize the decrease of sex hormone induced by BPA, but the improvement of high concentration of vitamin E is relatively significant.
【作者单位】： 上海交通大学医学院附属新华医院环境与儿童健康教育部和上海市重点实验室;
【基金】：国家重点基础研究发展计划(“973”计划)(2014CB943300) 上海市教育委员会高峰高原学科建设计划(20152220)~~
【分类号】：R114
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