ATP生物荧光技术在口腔治疗用水污染状况现场快速检测中的应用初探

发布时间：2018-07-16 20:14

【摘要】：目的探讨ATP生物荧光技术在口腔治疗用水污染状况现场快速检测中的可行性。方法采集48家相同规模的医疗机构口腔科共720份治疗用水,按照口腔科综合治疗台供水水源类型不同分为4组,检测水中细菌菌落总数,同时用专用检测棒检测ATP荧光值。分析两种方法的相关性,建立数学模型,得出现场快速检测时ATP荧光法限值,并用该限值分析在其他医疗机构口腔科采集的治疗用水水样,验证试验的可行性。结果不同供水水源的口腔治疗用水均存在污染。4组不同水源的水样ATP荧光法检测结果与实验室平皿计数法定量检测结果均有较高的相关性(r=0.32,P0.01)。4组数据可拟合一般线性方程,y=0.869X-0.495(R~2=0.819,P=0.00)。参照生活饮用水中细菌总数应100CFU/mL的标准,现场快速检测时的ATP荧光法限值(RLU)分别为787。用该限值分析2015年在其他医疗机构口腔科采集的120份水样,试验模型总体灵敏度83.87%,特异度87.64%,Kappa值0.673。结论 ATP荧光法不能完全替代传统微生物检测法,但可与实验室方法相互配合作为口腔治疗用水污染状况现场快速检测和筛查的方法,提高监督执法效率。
[Abstract]:Objective to investigate the feasibility of ATP bioluminescence technique for rapid detection of water pollution in oral treatment. Methods A total of 720 water samples were collected from the stomatology department of 48 medical institutions of the same size. According to the types of water supply in the comprehensive treatment table of stomatology department, they were divided into 4 groups. The total bacterial colony in the water was detected, and the ATP fluorescence value was detected with the special detection rod. The correlation between the two methods was analyzed and a mathematical model was established. The limit value of ATP fluorescence method was obtained when rapid detection was carried out in the field, and the water samples collected in the stomatology department of other medical institutions were analyzed to verify the feasibility of the test. Results there was significant correlation between ATP fluorescence method and laboratory plate counting method (rn 0.32 P 0.01). 4 groups of water samples were polluted in different water supply sources. 4 groups of data could fit the general linear equation of 0.869X-0.495 (Rhizoma Rhizoma 0.819P0.00). According to the standard of 100 CFU / mL for total bacteria in drinking water, the limit value of ATP fluorescence method (RLU) for field rapid detection is 787, respectively. 120 water samples collected in the stomatology department of other medical institutions in 2015 were analyzed with this limit. The overall sensitivity of the model was 83.87 and the specificity was 87.64 and the Kappa value was 0.673. Conclusion ATP fluorescence method can not completely replace the traditional microbial detection method, but it can be used as a rapid detection and screening method for the contamination of oral water in the field and improve the efficiency of supervision and law enforcement.
【作者单位】：宜昌市卫生计生综合监督执法局;
【基金】：湖北省预防医学会“消毒领域科研创新”项目(XKC2015-09)
【分类号】：R123;R115

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