萋-尼染色导致的总挥发性有机物污染及其控制方法

发布时间：2018-07-20 19:17

【摘要】：目的建立一种改良抗酸染色冷染方法,用以控制实验室总挥发性有机物(total volatile organic compound,TVOC)污染。方法采用TVOC检测仪定量测定实验室内空气中的苯系物和TVOC污染程度;以卡介苗(bacillecalmette-guerin,BCG)为染色标本,配制含有1%Triton X-100标本稀释液和初染液,建立不用加温助染的抗酸染色冷染法替代传统抗酸染色法。结果传统萋-尼抗酸染色时,室内TVOC污染在12 min时间点即高达1 014 ppb(约1 mg/m~3),成倍超过国家室内卫生标准(GB50325-2010)规定值(500 ppb,即0.5 mg/m~3);同样条件下,用改良冷染法染色期间,室内TVOC量有所增加,但在试验期间最高值为387 ppb,保持在国家室内卫生标准容许限度以下;利用Triton X-100建立的改良抗酸冷染法具有抗酸染色特性,其对抗酸杆菌的可检测度为3×10~(-6)~6×10~(-5)CFU/mL。结论抗酸染色冷染法可用于替代传统萋-尼抗酸染色,从而控制实验室的TVOC污染问题。
[Abstract]:Objective to establish an improved cold dyeing method for acid fast dyeing to control the pollution of total volatile organic compounds (total volatile organic) in laboratory. Methods the contamination of benzene series and TVOC in the air of the laboratory was determined quantitatively by using TVOC detector, and the diluted solution and the initial dye solution containing 1 Triton X-100 were prepared with Bacillus Calmette-Guerin BCG as the staining specimen. An acid fast cold dyeing method was established to replace the traditional acid fast dyeing method. Results the indoor TVOC pollution was as high as 1 014 ppb (about 1 mg/m~3) at 12 min, which was twice higher than the specified value of the National Indoor Hygiene Standard (GB50325-2010) (500ppb, i.e. 0.5 mg/m~3) at 12 min, under the same conditions, during the period of dyeing with modified cold dyeing method, the indoor TVOC pollution was as high as 1 014 ppb (about 1 mg/m~3) at the time point of 12 min. The amount of indoor TVOC increased, but the highest value was 387 ppb during the trial period, which kept below the allowable limit of national indoor hygiene standard. The modified acid-fast cold dyeing method established by Triton X-100 had the characteristics of acid-fast dyeing, and the detectable degree of acid-resistant bacillus was 3 脳 10 ~ (-6) ~ (-6) ~ (-6) ~ (-5) CFU ~ (-5) CFU / mL. Conclusion the cold dyeing method can be used to control TVOC pollution in laboratory.
【作者单位】：第三军医大学新桥医院感染控制科;第三军医大学基础医学部微生物学教研室重庆市微生物工程实验室;
【基金】：国家自然科学基金面上项目(31570173)~~
【分类号】：R122

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