石油工人职业紧张致高血压队列及相关分子生物学研究
发布时间：2018-07-21 15:21
【摘要】：目的:从队列研究、分子生物学方面探索石油工人职业紧张与高血压的关系,为防治石油工人高血压提供科学依据。方法:本研究采用队列研究的方法,研究基线队列为本课题组于2013年建立的新疆克拉玛依石油工人队列,前瞻性随访3年,最终1,233人进入队列。采用职业紧张量表(OSI-R)和付出-回报失衡问卷(ERI)进行流行病学调查。采用病例对照研究,选择DNA合格的样本进行聚合酶链式反应限制性片段长度多态性(PCR-RFLP)检测ADD1基因、β2-AR基因,高血压病例组、对照组各176人。从病例组中筛选出低、高度职业紧张各3例进行450K甲基化芯片测定。结果:1)本研究的基线队列共计1,535人,剔除基线时已患高血压者136人,问卷数据无效者22人。2016年随访终止时,共失访144人,最终1,233人进入队列;2)基线队列石油工人得分与国内职业紧张常模得分相比,除任务不适外,职业任务问卷、个体紧张反应问卷和个体应对资源问卷及其下属的子项得分差异均有统计学意义(P0.05)。基线队列与随访队列OSI-R得分相比,职业任务问卷、个体紧张反应问卷及个体应对资源问卷得分差异均有统计学意义(P0.05)。不同性别、族别、工龄、倒班情况、文化程度、工种、婚姻状况及收入的低、中、高三种职业紧张程度不同(P0.05),男性、汉族、工龄15年、钻井工职业紧张程度较高。随访至2016年,共315人在随访期间患高血压,累积发病率为22.76%。付出回报失衡的工人职业紧张程度较高(P0.05)。病例组与对照组ERI得分的差异有统计学意义(P0.05)。总胆固醇、总胆红素和总蛋白在病例组与对照组间差异无统计学意义(P0.05);3)ADD1基因rs17833172位点病例组与对照组差异有统计学意义(P0.05)。ADD1基因rs17833171和rs4961两位点间A-T单体型为高血压危险因素单体型,G-G单体型为高血压保护因素单体型(P0.05)。年龄、工种为钻井工或采油工、吸烟、rs17833172位点AG+GG基因型、高BMI、高空腹血糖、高乳酸脱氢酶和高尿酸是高血压的危险因素。rs4961位点与rs1042717位点之间的交互作用是高血压的保护因素。职业紧张与rs17833172位点、付出-回报与rs4961位点之间的交互作用是高血压产生的保护因素,付出-回报与rs17833172位点之间的交互作用是导致高血压产生的危险因素。450K甲基化芯片筛选出高度职业紧张组共有11,977个差异甲基化位点,低度职业紧张组共有8,312个差异甲基化位点,不同的染色体所含差异甲基化基因位点不同,ISLANDInd区域差异甲基化基因最多。结论:1)克拉玛依石油工人队列存在长期的职业紧张情况,暴露因素相对稳定;2)石油工人职业紧张程度较高,钻井工紧张程度最高。付出大于回报者职业紧张程度较高。BMI、甘油三脂、低密度脂蛋白、乳酸脱氢酶等指标影响着高血压的产生;3)ADD1基因rs17833172位点GG基因型是高血压的易感基因型。ADD1基因rs17833171和rs4961两位点间A-T单体型为高血压危险因素单体型,G-G单体型为高血压保护因素单体型。年龄大、工种为钻井工或采油工、吸烟、rs17833172位点AG+GG基因型、高BMI、高空腹血糖、高乳酸脱氢酶和高尿酸能够导致高血压的产生。个体应对资源得分高、携带rs4961位点GT+TT基因型是高血压的保护因素。各基因位点之间、基因与环境之间交互作用能够导致高血压的产生。450K甲基化芯片筛选出高度职业紧张组存在较多的低甲基化位点,低度职业紧张组高甲基化位点较多,不同的染色体所含差异甲基化位点不同,ISLANDInd区域差异甲基化基因最多,高血压与职业紧张在全基因组层面上存在甲基化表达谱的差异。
[Abstract]:Objective: To explore the relationship between occupational stress and hypertension in oil workers from cohort study and molecular biology, and to provide a scientific basis for the prevention and treatment of hypertension in oil workers. Methods: This study was conducted by cohort study, and the baseline cohort was established in 2013 in Karamay, Xinjiang, with a prospective follow-up of 3 years. The final 1233 people entered the cohort. The epidemiological survey was conducted using the Occupational Stress Scale (OSI-R) and the pay return imbalance questionnaire (ERI). A case-control study was used to select the DNA qualified samples for the polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) for the detection of the ADD1 gene, the beta 2-AR gene, the hypertension case group and the control group of 176 persons each. From the case group, 3 cases of low and high occupational stress were screened by 450K methylation chip. Results: 1) the baseline cohort of this study was 1535 people, 136 people had been suffering from hypertension at baseline and 22 were followed up at the end of the follow-up of 22.2016, the final 1233 people entered the queue; 2) the baseline queue of oil workers was scored. Compared with the domestic occupational stress norm score, the occupational task questionnaire, the individual stressful response questionnaire, the individual coping resource questionnaire and the sub item scores of the subordinates were statistically significant (P0.05). The baseline cohort was compared with the OSI-R scores in the follow-up cohort, and the occupational task questionnaire, individual stressful response questionnaire and individual coping funds were compared. The difference in the score of the source questionnaire was statistically significant (P0.05). There was a high degree of tension between the three types of occupational stress (P0.05), male, Han, 15 years, and a high degree of tension in the drilling job. 315 people were followed up to 2016, a total of 315 people suffered high blood pressure during the follow-up period. The occupational stress of the workers with the cumulative incidence of 22.76%. was high (P0.05). The difference in ERI scores between the case group and the control group was statistically significant (P0.05). There was no significant difference between the total cholesterol, total bilirubin and total protein in the case group and the control group (P0.05); 3) the ADD1 gene rs17833172 loci group was poor with the control group. The A-T haplotype between rs17833171 and rs4961 two loci of.ADD1 gene was statistically significant (A-T), and G-G haplotype was a type of hypertension protective factor haplotype (P0.05). Age, age, drillers or oil workers, smoking, AG+GG based rs17833172 loci, high BMI, high altitude abdominal blood glucose, high lactate dehydrogenase and high uric acid. The interaction between the.Rs4961 locus and the rs1042717 locus, the risk factor for hypertension, is a protective factor for hypertension. The interaction between occupational stress and rs17833172, the interaction between pay return and rs4961 loci is a protective factor for hypertension, and the interaction between pay and return and rs17833172 sites leads to high blood pressure The risk factors of.450K methylation chips were screened for 11977 different methylation sites in the high occupational stress group. There were 8312 difference methylation sites in the low occupational stress group, different methylation locus in different chromosomes, and the most methylation in the ISLANDInd region. Conclusion: 1) Karamay oil workers' queue There were long-term occupational stress conditions and relatively stable exposure factors; 2) oil workers' occupational stress was higher and the drillers had the highest tension. The higher occupational tension of.BMI, glycerol three fat, low density lipoprotein, lactate dehydrogenase and other indexes affecting the production of hypertension; 3) the rs17833172 locus GG genotype of ADD1 gene. The susceptibility gene type.ADD1 gene rs17833171 and rs4961 two loci between the hypertension and the A-T haplotype are the haplotype of hypertension risk factor, and the G-G haplotype is the haplotype of the hypertension protection factor. The age is large, the type of work is the drilling worker or the oil worker, the smoking, the rs17833172 locus AG+GG genotype, the high BMI, the high altitude abdominal blood sugar, the high lactate dehydrogenase and the high uric acid energy. High blood pressure can lead to high blood pressure. Individual response to resources is high, and rs4961 locus GT+TT genotype is a protective factor for hypertension. The interaction between gene loci, gene and environment can lead to the production of hypertension by.450K methylation chips to screen high occupational stress groups with more hypomethylation sites and low degree occupations. There are more methylation sites in the tension group, different methylation sites in different chromosomes, the most methylation genes in the ISLANDInd region, and the difference in the methylation expression profiles at the whole genome level between hypertension and occupational stress.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R135
本文编号：2135950
[Abstract]:Objective: To explore the relationship between occupational stress and hypertension in oil workers from cohort study and molecular biology, and to provide a scientific basis for the prevention and treatment of hypertension in oil workers. Methods: This study was conducted by cohort study, and the baseline cohort was established in 2013 in Karamay, Xinjiang, with a prospective follow-up of 3 years. The final 1233 people entered the cohort. The epidemiological survey was conducted using the Occupational Stress Scale (OSI-R) and the pay return imbalance questionnaire (ERI). A case-control study was used to select the DNA qualified samples for the polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) for the detection of the ADD1 gene, the beta 2-AR gene, the hypertension case group and the control group of 176 persons each. From the case group, 3 cases of low and high occupational stress were screened by 450K methylation chip. Results: 1) the baseline cohort of this study was 1535 people, 136 people had been suffering from hypertension at baseline and 22 were followed up at the end of the follow-up of 22.2016, the final 1233 people entered the queue; 2) the baseline queue of oil workers was scored. Compared with the domestic occupational stress norm score, the occupational task questionnaire, the individual stressful response questionnaire, the individual coping resource questionnaire and the sub item scores of the subordinates were statistically significant (P0.05). The baseline cohort was compared with the OSI-R scores in the follow-up cohort, and the occupational task questionnaire, individual stressful response questionnaire and individual coping funds were compared. The difference in the score of the source questionnaire was statistically significant (P0.05). There was a high degree of tension between the three types of occupational stress (P0.05), male, Han, 15 years, and a high degree of tension in the drilling job. 315 people were followed up to 2016, a total of 315 people suffered high blood pressure during the follow-up period. The occupational stress of the workers with the cumulative incidence of 22.76%. was high (P0.05). The difference in ERI scores between the case group and the control group was statistically significant (P0.05). There was no significant difference between the total cholesterol, total bilirubin and total protein in the case group and the control group (P0.05); 3) the ADD1 gene rs17833172 loci group was poor with the control group. The A-T haplotype between rs17833171 and rs4961 two loci of.ADD1 gene was statistically significant (A-T), and G-G haplotype was a type of hypertension protective factor haplotype (P0.05). Age, age, drillers or oil workers, smoking, AG+GG based rs17833172 loci, high BMI, high altitude abdominal blood glucose, high lactate dehydrogenase and high uric acid. The interaction between the.Rs4961 locus and the rs1042717 locus, the risk factor for hypertension, is a protective factor for hypertension. The interaction between occupational stress and rs17833172, the interaction between pay return and rs4961 loci is a protective factor for hypertension, and the interaction between pay and return and rs17833172 sites leads to high blood pressure The risk factors of.450K methylation chips were screened for 11977 different methylation sites in the high occupational stress group. There were 8312 difference methylation sites in the low occupational stress group, different methylation locus in different chromosomes, and the most methylation in the ISLANDInd region. Conclusion: 1) Karamay oil workers' queue There were long-term occupational stress conditions and relatively stable exposure factors; 2) oil workers' occupational stress was higher and the drillers had the highest tension. The higher occupational tension of.BMI, glycerol three fat, low density lipoprotein, lactate dehydrogenase and other indexes affecting the production of hypertension; 3) the rs17833172 locus GG genotype of ADD1 gene. The susceptibility gene type.ADD1 gene rs17833171 and rs4961 two loci between the hypertension and the A-T haplotype are the haplotype of hypertension risk factor, and the G-G haplotype is the haplotype of the hypertension protection factor. The age is large, the type of work is the drilling worker or the oil worker, the smoking, the rs17833172 locus AG+GG genotype, the high BMI, the high altitude abdominal blood sugar, the high lactate dehydrogenase and the high uric acid energy. High blood pressure can lead to high blood pressure. Individual response to resources is high, and rs4961 locus GT+TT genotype is a protective factor for hypertension. The interaction between gene loci, gene and environment can lead to the production of hypertension by.450K methylation chips to screen high occupational stress groups with more hypomethylation sites and low degree occupations. There are more methylation sites in the tension group, different methylation sites in different chromosomes, the most methylation genes in the ISLANDInd region, and the difference in the methylation expression profiles at the whole genome level between hypertension and occupational stress.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R135
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