表面等离子共振传感器检测3种果汁中亚胺硫磷残留

发布时间：2018-07-25 06:14

【摘要】：基于亚胺硫磷多克隆抗体,建立苹果汁、桃汁、橙汁中亚胺硫磷残留的表面等离子共振(surface plasmon resonance,SPR)传感器检测方法。采用磷酸盐缓冲液直接稀释样品10倍后,基本消除基质影响,加标回收率为84.88%~104.69%,变异系数低于6.70%,样品的最低检出限为0.1μg/L,与高效液相色谱检测结果相关系数为0.992,所建立的SPR检测方法达到了快速准确检测的要求。SPR反应芯片对亚胺硫磷的检测时间为30 min,且在3 d内可反复检测30次,可有效降低检测成本。通过与同源抗体建立的酶联免疫方法比较发现:SPR方法在检测限、精确度、灵敏度等方面有着一定优势,但对于单残留样品的检测,在时间上不具有明显优势,更适合于高通量、多残留的检测。
[Abstract]:Based on polyclonal antibody of iminphosphos, a surface plasmon resonance (SPR) sensor was developed for the detection of iminphosphos residues in apple juice, peach juice and orange juice. After directly diluting the sample with phosphate buffer solution for 10 times, the matrix effect was basically eliminated. The recovery rate was 84.88% 104.69, the coefficient of variation was lower than 6.70, the minimum detection limit of the sample was 0.1 渭 g / L, and the correlation coefficient with the result of high performance liquid chromatography was 0.992. The established SPR detection method reached the requirement of rapid and accurate detection. The detection time of sulfur and phosphorus was 30 mins and 30 times in 3 days. It can effectively reduce the cost of detection. By comparing with the enzyme-linked immunosorbent assay (Elisa) established by homologous antibodies, it was found that the detection limit, accuracy, sensitivity and so on had some advantages, but there was no obvious advantage in time for the detection of single residual samples, which was more suitable for high throughput. Multiresidue detection.
【作者单位】：天津师范大学生命科学学院天津市动植物抗性重点实验室;天津市林业果树研究所;
【基金】：国家自然科学基金青年科学基金项目(31301487) “十二五”国家科技支撑计划项目(2014BAD04B03) 天津市自然科学基金项目(13JCQNJC14800)
【分类号】：R155.5

【参考文献】