蛋氨酸羟基类似物对肉鸡肠道氧化还原状态和骨骼发育的影响
发布时间：2018-07-28 13:10
【摘要】：肠道和骨骼是机体重要的消化吸收场所和支柱器官，维护其健康稳态对机体生长发育具有重要意义。蛋氨酸(MET)是人体必需氨基酸，其替代源包括蛋氨酸羟基类似物(HMTBA)和蛋氨酸羟基类似物钙盐(HMTB-Ca)。由于结构上的差异，HMTBA和HMTB-Ca相较于DL-蛋氨酸(DLM)可能具有较强的抗氧化活性，对维护肠道氧化还原稳态以及促进骨骼发育具有积极作用。本论文比较分析了DLM、HMTBA和HMTB-Ca的体外抗氧化活性；并以生长周期短，代谢强度大的肉鸡作为研究对象，研究了不同水平蛋氨酸源对肉鸡生长性能、营养物质消化吸收、肠道氧化还原状态以及骨骼发育的影响，初步探讨了其可能的抗氧化作用机制。 体外部分：考察了DLM、HMTBA和HMTB-Ca对O-2·清除率和对小鼠肝脏质过氧化抑制率；体内部分：210只1日龄雄性罗斯肉鸡，按体重随机分为7组：对照组（无外源性蛋氨酸源添加）、试验组分别饲喂添加DLM、HMTBA及HMTB-Ca的试验饲粮，添加水平分别为0.2%和0.3%(前期)，0.1%和0.2%(后期)。于实验第21天和46天采样，分析各组实验动物生长性能、对营养物质消化吸收率、肠道氧化还原状态及骨骼发育相关指标，并考察了肠道及骨骼组织相关基因的mRNA表达水平。 本论文主要研究结果如下：(1)体外O-2·清除能力：HMTBADLMHMTB-Ca；体外小鼠肝脏脂质过氧化抑制率：HMTBAHMTB-CaDLM。(2)肉鸡饲喂三种蛋氨酸源21天和46天均可提高动物日增重和采食量，且有一定的剂量-效应关系；与DLM组相比，肉鸡饲喂HMTBA和HMTB-Ca21天可显著提高日增重和采食量(P0.05)，饲喂46天可显著提高日增重(P0.05)，且高剂量HMTBA和HMTB-Ca组效果最为显著。(3)与DLM组相比，，高剂量的HMTBA组和HMTB-Ca组肠道淀粉酶和脂肪酶活力显著增强(P0.05)，HMTB-Ca组对营养物质的表观消化率提高；46天肉鸡饲喂高剂量HMTBA和HMTB-Ca可显著提高十二指肠和空肠绒毛长度/隐窝深度比值(V/C)(P0.05)，降低生长抑素(SS)分泌水平。(4)肉鸡饲喂HMTBA和HMTB-Ca显著提高十二指肠和空肠GSH/GSSG比值和CAT活性(P0.05)，降低MDA水平(P0.05)；上调十二指肠和空肠抗氧化相关基因NRF1、SOD1、SOD2、GST、PGC-1α、MT1mRNA表达水平。(5)与DLM组相比，肉鸡饲喂高剂量HMTBA和HMTB-Ca可显著提高胫骨、股骨骨骼强度和骨骼指数(P0.05)，饲喂HMTB-Ca可显著提高胫骨、股骨钙、磷含量(P0.05)，并且显著下调骨分解关键基因MMP2、MMP9mRNA表达水平(P0.05)。
[Abstract]:Intestinal tract and bone are important digestive and absorption sites and pillar organs, and maintaining their health and steady-state is of great significance to the growth and development of the body. Methionine (MET) is an essential amino acid in human body. Its alternative sources include methionine hydroxyl analogues (HMTBA) and methionine hydroxyl analogues (HMTB-Ca). Compared with DL-methionine (DLM), HMTBA and HMTB-Ca may have stronger antioxidant activity than DL-methionine (DLM), and play a positive role in maintaining redox homeostasis and promoting bone development. In this paper, the antioxidant activities of DLM-HMTBA and HMTB-Ca in vitro were comparatively analyzed, and the growth performance, digestibility and absorption of nutrients of broilers with different levels of methionine were studied by using the broilers with short growth cycle and high metabolic intensity. The effects of intestinal redox state and bone development on the mechanism of antioxidation were preliminarily discussed. In vitro, the effects of DLMN HMTBA and HMTB-Ca on the scavenging rate of O-2 and the inhibition rate of liver peroxidation in mice were investigated, and 210 one-day-old male Roth broilers were selected. According to body weight, the rats were randomly divided into 7 groups: control group (without exogenous methionine source), the experimental diet supplemented with DLMM-HMTBA and HMTB-Ca, the supplementation levels were 0.2% and 0.3% (Prophase) and 0.2% (later), respectively. On the 21st and 46th days of the experiment, the growth performance, the digestibility of nutrients, the redox state of the intestine and the related indexes of bone development were analyzed, and the mRNA expression level of the genes related to the intestinal tract and bone tissue were investigated. The main results of this study were as follows: (1) the in vitro O-2 scavenging capacity of 1: HMTBADLMHMTB-Caand the inhibition rate of lipid peroxidation in mice liver in vitro. (2) three methionine sources fed to broilers for 21 and 46 days could increase the daily gain and feed intake. Compared with DLM group, HMTBA and HMTB-Ca21 increased daily gain and feed intake significantly (P0.05), fed 46 days significantly increased daily gain (P0.05), and the effect of high dose HMTBA and HMTB-Ca groups was the most significant. (3) compared with DLM group, the effect of high dose HMTBA and HMTB-Ca groups was the most significant. The activities of amylase and lipase in high dose HMTBA group and HMTB-Ca group were significantly increased (P0.05) the apparent digestibility of nutrients was increased in HMTB-Ca group. High-dose HMTBA and HMTB-Ca significantly increased the ratio of villi length to crypt depth in duodenum and jejunum (P0.05) and decreased the level of somatostatin (SS) secretion. (4) HMTBA and HMTB-Ca significantly increased GSH/GSSG ratio of duodenum and jejunum. The activity of CAT (P0.05) decreased the level of MDA (P0.05). (5) compared with DLM group, high dose of HMTBA and HMTB-Ca significantly increased tibia, bone strength and bone index (P0.05), and fed HMTB-Ca significantly increased tibia calcium. P content (P0.05), and significantly down-regulated the expression level of MMP2 and MMP9 mRNA (P0.05).
【学位授予单位】：江南大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R151.1
本文编号：2150250
[Abstract]:Intestinal tract and bone are important digestive and absorption sites and pillar organs, and maintaining their health and steady-state is of great significance to the growth and development of the body. Methionine (MET) is an essential amino acid in human body. Its alternative sources include methionine hydroxyl analogues (HMTBA) and methionine hydroxyl analogues (HMTB-Ca). Compared with DL-methionine (DLM), HMTBA and HMTB-Ca may have stronger antioxidant activity than DL-methionine (DLM), and play a positive role in maintaining redox homeostasis and promoting bone development. In this paper, the antioxidant activities of DLM-HMTBA and HMTB-Ca in vitro were comparatively analyzed, and the growth performance, digestibility and absorption of nutrients of broilers with different levels of methionine were studied by using the broilers with short growth cycle and high metabolic intensity. The effects of intestinal redox state and bone development on the mechanism of antioxidation were preliminarily discussed. In vitro, the effects of DLMN HMTBA and HMTB-Ca on the scavenging rate of O-2 and the inhibition rate of liver peroxidation in mice were investigated, and 210 one-day-old male Roth broilers were selected. According to body weight, the rats were randomly divided into 7 groups: control group (without exogenous methionine source), the experimental diet supplemented with DLMM-HMTBA and HMTB-Ca, the supplementation levels were 0.2% and 0.3% (Prophase) and 0.2% (later), respectively. On the 21st and 46th days of the experiment, the growth performance, the digestibility of nutrients, the redox state of the intestine and the related indexes of bone development were analyzed, and the mRNA expression level of the genes related to the intestinal tract and bone tissue were investigated. The main results of this study were as follows: (1) the in vitro O-2 scavenging capacity of 1: HMTBADLMHMTB-Caand the inhibition rate of lipid peroxidation in mice liver in vitro. (2) three methionine sources fed to broilers for 21 and 46 days could increase the daily gain and feed intake. Compared with DLM group, HMTBA and HMTB-Ca21 increased daily gain and feed intake significantly (P0.05), fed 46 days significantly increased daily gain (P0.05), and the effect of high dose HMTBA and HMTB-Ca groups was the most significant. (3) compared with DLM group, the effect of high dose HMTBA and HMTB-Ca groups was the most significant. The activities of amylase and lipase in high dose HMTBA group and HMTB-Ca group were significantly increased (P0.05) the apparent digestibility of nutrients was increased in HMTB-Ca group. High-dose HMTBA and HMTB-Ca significantly increased the ratio of villi length to crypt depth in duodenum and jejunum (P0.05) and decreased the level of somatostatin (SS) secretion. (4) HMTBA and HMTB-Ca significantly increased GSH/GSSG ratio of duodenum and jejunum. The activity of CAT (P0.05) decreased the level of MDA (P0.05). (5) compared with DLM group, high dose of HMTBA and HMTB-Ca significantly increased tibia, bone strength and bone index (P0.05), and fed HMTB-Ca significantly increased tibia calcium. P content (P0.05), and significantly down-regulated the expression level of MMP2 and MMP9 mRNA (P0.05).
【学位授予单位】：江南大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R151.1
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本文编号：2150250
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