不同孕期孕鼠喂饲超剂量食品添加剂对胎鼠的发育、胎盘及脑组织c-fos基因表达的影响

发布时间：2018-08-06 20:10

【摘要】：目的研究在不同孕期的大鼠联合应用的超剂量的食品添加剂时对胎鼠的发育、胎盘影响和胎鼠脑组织C-fos基因表达关系。从而探讨联合应用超剂量食品添加剂对各个孕期胎鼠胚胎的毒性,表现形式和胎鼠脑组织C-fos基因的表达关系。方法建立孕期联合应用超剂量食品添加剂模型,利用HE染色及逆转录多聚酶链反应检测不同孕期胎鼠的胎盘组织、脑组织的病理变化及海马中c-fos基因的表达。结果(1)A组(2-7天联合应用超剂量食品添加剂组)的吸收胎及死胎比率为15.58%(12/77),B组(8-13天联合应用超剂量食品添加剂组)的吸收胎及死胎比率为4.87%(4/82),同C组(14-19天联合应用超剂量食品添加剂组)1.22%(1/3)比较均明显升高,差异均有显著性(P0.01、P0.05)；A组及B组的吸收胎及死胎比率与D组(对照组)1.14%相比较亦有类似结果(P0.01及P0.05);而C组及D组比较差异无显著性(P0.05)。(2)A组胎鼠的平均体重及平均胎盘重为3.75±0.12mg、0.593±0.183mg,B组为3.42±0.10mg、0.574±0.348mg,明显低于C组胎鼠的平均体重及胎盘重(4.01±0.09mg、0.623±0.214mg),差异有显著性(P0.05)；A组及B组胎仔的平均体重及胎盘重与D组(4.12±0.43mg、0.654±0.212mg)相比较亦有类似结果(P均0.05)；A组与B组相比较有统计学意义(P0.05),C组及D组比较差异无显著性(P0.05)。(3)B组的平均身长及尾长(3.47±0.14mm、1.02±0.56mm)与其它各组相比有统计学意义(P0.05),其它各组之间比较差异无显著性。(4)B组IUGR发生率为28.2%(22/78),明显高于其它三组(A组：8/65=12.3%；C组：3/84=3.57%；D组：1/86=1.16%),差异有显著性(P0.05)；A组IUGR发生率与C组及D组的IUGR发生率相比较,差异有显著性(P0.05)；C组与D组的IUGR发生率相比较,差异无显著性(P0.05)(5)B组胎鼠的胎盘及脑组织病理改变最为显著。(6)B组的脑组织c-fos基因表达(0.42±0.09)与A组(0.21±0.08)、C组(0.10±0.04)及D组(0.06±0.03)相比有统计学意义(P0.05)。结论鼠联合应用超剂量食品添加剂可导致胚胎毒性,其影响程度与孕期有关。(1)超剂量食品添加剂对宫内胚胎发育有时间-效应关系。(2)孕期联合应用超剂量食品添加剂可致妊娠大鼠胎盘组织及脑组织发生病理学改变,以孕8-13天组病理改变最为明显。(3)孕期联合应用超剂量食品添加剂可致胎鼠脑组织c-fos mRNA过度表达,并以孕8-13天组表达最为明显。这又一次说明了鼠妊娠8-13天即人的妊娠早期是超剂量食品添加剂对脑组织损伤的重要的时间窗,为我们人为的干预食品添加剂的使用起了指导作用。
[Abstract]:Objective to study the relationship between the development of fetal rat placenta and the expression of C-fos gene in fetal brain tissue. To explore the relationship between the toxicity and the expression of C-fos gene in the fetal brain tissue of fetal rats during pregnancy after the combined use of high dose food additives. Methods the model of superdose food additive was established during pregnancy. The pathological changes of placenta and brain tissues and the expression of c-fos gene in hippocampus were detected by HE staining and reverse transcriptase polymerase chain reaction (RT-PCR). Results (1) the ratio of resorption and stillbirth in group A (2-7 days combined with overdose food additive) was 15.58% (12 / 77) and that in group B (8-13 days) was 4.87% (4 / 82), and that in group C (14-19 days) was 4.87% (4 / 82). In the food additive group, 1.22% (1 / 3) was significantly higher than that in the control group. The difference was significant (P0.01P 0.05). The ratio of absorbed fetus and dead fetus in group A and group B was similar to that in group D (P 0.01 and P 0.05), but there was no significant difference between group C and group D (P0.05). The average body weight and placental weight of group A were 3.75 卤0.12 mg / kg and 0.593 卤0.183 mg / g respectively, which were significantly higher than those of group B (3.42 卤0.10 mg / g vs 0.574 卤0.348 mg / g). The average body weight and placental weight of group C were significantly lower than those of group C (4.01 卤0.09 mg / kg, 0.623 卤0.214mg) (P0.05). The average body weight and placental weight of group A and group B were similar to those of group D (4.12 卤0.43 mg / kg, 0.654 卤0.212mg). There was no significant difference between group A and group B (P0.05). There was no significant difference between group C and group D (P0.05). The average body length and tail length of group B (3.47 卤0.14mm-1. 02 卤0.56mm) were significantly higher than those of other groups (P0.05), but there was no significant difference between other groups. (4) the incidence of IUGR in group B was not significantly different. The birth rate was 28.2% (22 / 78), which was significantly higher than that of the other three groups (group A: 8 / 65) 12.3; The incidence of IUGR in group A was significantly higher than that in group C and group D (P0.05). The incidence of IUGR in group C was significantly higher than that in group C and group D (P0.05). (6) the expression of c-fos gene in group B (0.42 卤0.09) was significantly higher than that in group A (0.21 卤0.08) and group C (0.10 卤0.04) and group D (0.06 卤0.03) (P0.05). Conclusion combined use of high dose food additives in mice can lead to embryotoxicity. The degree of influence was related to pregnancy. (1) there was a time-effect relationship between overdose food additives and intrauterine embryo development. (2) the pathological changes of placenta and brain tissues of pregnant rats were induced by the combination of overdose food additives during pregnancy. The pathological changes were most obvious in the 8-13 day group. (3) the excessive expression of c-fos mRNA in fetal brain tissue was induced by the combination of high dose food additive during pregnancy, and the expression was the most obvious in the 8-13 day group. This once again shows that the early stage of human pregnancy is an important time window for brain tissue damage caused by overdose food additives, which provides guidance for us to interfere with the use of food additives.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R155.5

【相似文献】