碘丙炔醇丁基氨甲酸酯醇溶液杀菌效果研究

发布时间：2018-08-24 17:43

【摘要】：目的研究碘丙炔醇丁基氨甲酸酯(IPBC)醇溶液的杀菌效果。方法参考2002年版《消毒技术规范》,用中和剂鉴定试验和悬液定量杀菌试验进行观察。结果含5 g/L卵磷脂和10 g/L吐温80的磷酸盐缓冲液(PBS)可有效中和实验条件下IPBC醇溶液对金黄色葡萄球菌和大肠杆菌的残留作用;1 600 mg/L、800 mg/L的IPBC醇溶液作用10 min,500 mg/L的IPBC醇溶液作用20 min,400 mg/L的IPBC醇溶液作用30 min,200 mg/L的IPBC醇溶液作用40 min,对金黄色葡萄球菌的杀灭对数值5.00,达到消毒水平;1 600 mg/L、800 mg/L的IPBC醇溶液作用10 min,500 mg/L、400 mg/L的IPBC醇溶液作用20 min,200 mg/L的IPBC醇溶液作用30 min,对大肠杆菌的杀灭对数值5.00,达到消毒水平。结论 500 mg/L IPBC醇溶液对金黄色葡萄球菌和大肠杆菌作用较短时间能达到消毒水平;金黄色葡萄球菌对IPBC醇溶液的抵抗力高于大肠杆菌。
[Abstract]:Objective to study the bactericidal effect of Ding Ji (IPBC) alcohol solution. Methods with reference to the Technical Specification for Disinfection in 2002, the neutralizer identification test and suspension quantitative germicidal test were used for observation. Results Phosphate buffer (PBS) containing 5 g / L lecithin and 10 g / L Tween 80 could effectively neutralize the residual effect of IPBC alcohol solution on Staphylococcus aureus and Escherichia coli in the presence of 1 600 mg/L,800 mg/L IPBC alcohol solution and 10 min,500 mg/L IPBC. Killing logarithm value of Staphylococcus aureus exposed to 20 min,400 mg/L IPBC Alcohol solution and 30 min,200 mg/L IPBC Alcohol solution with 40 min, reaching the disinfection level of 1 600 mg/L,800 mg/L IPBC Alcohol solution with 10 min,500 mg/L,400 mg/L IPBC Alcohol solution The killing logarithm of Escherichia coli exposed to 20 min,200 mg/L IPBC alcohol solution for 30 min, reached the disinfection level. Conclusion 500 mg/L IPBC alcohol solution can sterilize staphylococcus aureus and Escherichia coli in a short time, and the resistance of Staphylococcus aureus to IPBC alcohol solution is higher than that of Escherichia coli.
【作者单位】：四川大学华西公共卫生学院;深圳市南山区疾病预防控制中心;
【基金】：中华预防医学会“消毒领域科研创新”专项(2013DX001)
【分类号】：R187

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