AGT和NRG1基因多态性与职业紧张易感性关系的研究
发布时间：2018-08-26 07:23
【摘要】：【研究目的】 评价海洋石油作业工人的职业紧张水平，了解血管紧张素原（AGT）和神经调节因子（NRG1）的基因多态性，分析两基因各基因型与职业紧张分级的关系，探讨确定AGT和NRG1基因是否为职业紧张的易感基因。 【研究方法】 问卷调查：抽取胜利海洋采油厂包括EDC平台在内作业岗位的300名在岗员工，采用常规的职业紧张量表，进行问卷调查。问卷使用1998年修订版的中文版职业紧张量表，调查方式采用面对面方式，调查内容包括一般情况、饮食及生活习惯、家族史、疾病史、职业情况（如工龄、工种）和职业紧张状况。调查结果使用EpiData建库，用SPSS软件进行统计分析，得出采油厂工人职业紧张情况，并分析婚姻、学历，年龄和工龄等因素对职业紧张情况的影响。 实验验证：在取得工人知情同意的情况下，抽取上述工人静脉血，共300份。使用DNA快提试剂盒，提取工人DNA，设计AGT和NRG1两个基因的引物，使用PCR-RFLP法分析两基因的基因多态性。最后结合问卷调查结果，统计分析找出不同基因型对职业紧张指标的影响。 【研究结果】 问卷调查 1）紧张总体情况 本次调查对象职业任务紧张情况相对乐观，个体紧张反应表现在心理紧张反应和躯体紧张反应两个方面；在应对能力上，社会支持和理性处事是主要弱项。本次调查对象在职业任务和应对能力方面均优于常模。 2）职业紧张影响因素方面 本次研究从婚姻状况、学历情况、吸烟饮酒情况和年龄、工龄六个方面分析职业紧张的影响因素，发现不同年龄段对于紧张反应的应对能力不同，25到30岁的年龄段对于紧张的应对能力最差，吸烟饮酒情况可能会对职业紧张某些要素发挥作用，，婚姻情况和学历的高低则未表现出有意义的差异。 3）多元回归分析 通过多元回归分析发现，社会支持和责任感是对个人紧张反应影响最普遍的两个因素，社会支持与紧张反应呈负相关，社会支持度的提高可以极大缓解工人个体紧张反应情况，研究者在设计职业紧张干预措施时，应予以重点考虑。 实验验证 1）AGT和NRG1的基因多态性 AGT基因经过电泳后得出三个基因型，即：纯合子MM型（163bp）、TT型（140bp）和杂合子MT型（140bp、163bp）；NRG1基因经过电泳后也得出三各基因型，即：纯合子TT型（30bp、127bp）、GG型（60bp、127bp）和杂合子TG型（30bp、60bp、127bp）。经过检测，他们均符合遗传学Hardy-Weinberg平衡（P＞0.05），提示研究对象无偏倚。 2）职业紧张易感性 综合分析问卷和实验结果发现，血管紧张素原（AGT）和神经调节因子（NRG1）这两个基因的不同基因型携带者职业紧张指标之间均有显著的统计学差异(P＜0.05)，携带AGT(M235T M/T位点)T等位基因和NRG1(rs3924999G/T位点)T等位基因的研究对象职业任务阳性率和紧张反应阳性率显著高于AGT(M235T M/T位点)M等位基因和NRG1(rs3924999G/T位点)G等位基因的基因型携带者。 【研究结论】 1）当前海洋采油厂工人的个体紧张反应主要表现在心理紧张反应和躯体紧张反应方面，社会支持和理性处事是研究对象应对紧张的弱项，然而通过回归分析来看，这两项的重要程度不言而喻，特别是社会支持一项，将会很大程度上影响工人的职业紧张应对能力。 2）血管紧张素原AGT(M235T M/T位点)T等位基因和神经调节因子NRG1(rs3924999G/T位点)T等位基因可能是职业紧张的易感基因。
[Abstract]:[research purposes]
To evaluate the occupational stress level of offshore oil workers, to understand the genetic polymorphisms of angiotensinogen (AGT) and neuroregulatory factor (NRG1), to analyze the relationship between the two genotypes and occupational stress grading, and to determine whether AGT and NRG1 genes are susceptible to occupational stress.
[research methods]
Questionnaire survey: 300 on-the-job employees in Shengli offshore oil production plant, including EDC platform, were surveyed with the Occupational Stress Scale. The questionnaire used the Chinese version of Occupational Stress Scale revised in 1998. The survey was conducted face-to-face. The contents of the survey included general situation, diet and living habits, and family. Family history, disease history, occupational status (e.g. length of service, type of work) and occupational stress were analyzed by using EpiData database and SPSS software.
The results showed that 300 venous blood samples were obtained with the informed consent of the workers. The DNA of the workers was extracted by DNA rapid extraction kit, and the primers of AGT and NRG1 genes were designed. The polymorphisms of the two genes were analyzed by PCR-RFLP. Finally, the results of the questionnaire survey were used to analyze and find out the occupational stress caused by different genotypes. The influence of Zhang index.
[results]
Questionnaire investigation
1) the general situation of tension.
The respondents were relatively optimistic about occupational task stress, and their individual stress reaction was manifested in two aspects: psychological stress reaction and physical stress reaction. Social support and rational behavior were the main weaknesses in coping ability. The respondents were superior to the norm in occupational task and coping ability.
2) factors affecting occupational stress
This study analyzed the influencing factors of occupational stress from six aspects: marital status, educational background, smoking and drinking, age and length of service. It was found that different age groups had different coping abilities to stress reaction, the age group between 25 and 30 had the worst coping ability to stress, and smoking and drinking might exert some factors of occupational stress. There was no significant difference in marital status and educational background.
3) multiple regression analysis
Through multiple regression analysis, it is found that social support and sense of responsibility are the two most common factors affecting individual stress response. Social support is negatively correlated with stress response, and the improvement of social support can greatly alleviate individual stress response of workers.
experimental verification
1) genetic polymorphisms of AGT and NRG1
After electrophoresis, three genotypes of AGT gene were identified: homozygote MM (163 bp), TT (140 bp) and heterozygote MT (140 bp, 163 bp), and NRG1 gene was also identified as homozygote TT (30 bp, 127 bp), GG (60 bp, 127 bp) and heterozygote TG (30 bp, 60 bp, 127 bp). Inberg balance (P > 0.05) indicated that the subjects were not biased.
2) occupational stress and susceptibility
The results of questionnaire and experiment showed that there were significant differences in occupational stress between the carriers of angiotensinogen (AGT) and neuroregulatory factor (NRG1) genotypes (P The task-positive rate and the stress-positive rate were significantly higher than those of the genotype carriers of AGT (M235T M/T locus) M allele and NRG1 (rs3924999G/T locus) G allele.
[Conclusion]
1) At present, the individual stress reaction of the workers in offshore oil production plants is mainly manifested in the aspects of psychological stress reaction and physical stress reaction. Social support and rational behavior are the weaknesses of the research objects in dealing with stress. However, through regression analysis, the importance of these two factors is self-evident, especially social support, which will greatly affect. Workers' occupational stress coping ability.
2) Angiotensinogen AGT (M235T M/T locus) T allele and neuromodulator NRG1 (rs3924999G/T locus) T allele may be susceptible to occupational stress.
【学位授予单位】：济南大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R131
本文编号：2204141
[Abstract]:[research purposes]
To evaluate the occupational stress level of offshore oil workers, to understand the genetic polymorphisms of angiotensinogen (AGT) and neuroregulatory factor (NRG1), to analyze the relationship between the two genotypes and occupational stress grading, and to determine whether AGT and NRG1 genes are susceptible to occupational stress.
[research methods]
Questionnaire survey: 300 on-the-job employees in Shengli offshore oil production plant, including EDC platform, were surveyed with the Occupational Stress Scale. The questionnaire used the Chinese version of Occupational Stress Scale revised in 1998. The survey was conducted face-to-face. The contents of the survey included general situation, diet and living habits, and family. Family history, disease history, occupational status (e.g. length of service, type of work) and occupational stress were analyzed by using EpiData database and SPSS software.
The results showed that 300 venous blood samples were obtained with the informed consent of the workers. The DNA of the workers was extracted by DNA rapid extraction kit, and the primers of AGT and NRG1 genes were designed. The polymorphisms of the two genes were analyzed by PCR-RFLP. Finally, the results of the questionnaire survey were used to analyze and find out the occupational stress caused by different genotypes. The influence of Zhang index.
[results]
Questionnaire investigation
1) the general situation of tension.
The respondents were relatively optimistic about occupational task stress, and their individual stress reaction was manifested in two aspects: psychological stress reaction and physical stress reaction. Social support and rational behavior were the main weaknesses in coping ability. The respondents were superior to the norm in occupational task and coping ability.
2) factors affecting occupational stress
This study analyzed the influencing factors of occupational stress from six aspects: marital status, educational background, smoking and drinking, age and length of service. It was found that different age groups had different coping abilities to stress reaction, the age group between 25 and 30 had the worst coping ability to stress, and smoking and drinking might exert some factors of occupational stress. There was no significant difference in marital status and educational background.
3) multiple regression analysis
Through multiple regression analysis, it is found that social support and sense of responsibility are the two most common factors affecting individual stress response. Social support is negatively correlated with stress response, and the improvement of social support can greatly alleviate individual stress response of workers.
experimental verification
1) genetic polymorphisms of AGT and NRG1
After electrophoresis, three genotypes of AGT gene were identified: homozygote MM (163 bp), TT (140 bp) and heterozygote MT (140 bp, 163 bp), and NRG1 gene was also identified as homozygote TT (30 bp, 127 bp), GG (60 bp, 127 bp) and heterozygote TG (30 bp, 60 bp, 127 bp). Inberg balance (P > 0.05) indicated that the subjects were not biased.
2) occupational stress and susceptibility
The results of questionnaire and experiment showed that there were significant differences in occupational stress between the carriers of angiotensinogen (AGT) and neuroregulatory factor (NRG1) genotypes (P The task-positive rate and the stress-positive rate were significantly higher than those of the genotype carriers of AGT (M235T M/T locus) M allele and NRG1 (rs3924999G/T locus) G allele.
[Conclusion]
1) At present, the individual stress reaction of the workers in offshore oil production plants is mainly manifested in the aspects of psychological stress reaction and physical stress reaction. Social support and rational behavior are the weaknesses of the research objects in dealing with stress. However, through regression analysis, the importance of these two factors is self-evident, especially social support, which will greatly affect. Workers' occupational stress coping ability.
2) Angiotensinogen AGT (M235T M/T locus) T allele and neuromodulator NRG1 (rs3924999G/T locus) T allele may be susceptible to occupational stress.
【学位授予单位】：济南大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R131
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