煤焦沥青职业暴露人群DNA甲基化和端粒损伤研究

发布时间：2018-08-26 19:01

【摘要】：目的调查煤焦沥青职业接触人群p16、FHIT和RASSF1A基因启动子甲基化及端粒损伤情况,探讨煤焦沥青职业接触的效应标志。对象与方法 1.研究对象：180例煤焦沥青职业接触人群来自中部某碳素厂职工；145例对照人群来自同期体检正常的人群,此人群都是在本学校第一附属医院体检科进行体检。经其知情同意后,采集研究对象外周血,专业调查人员收集研究对象基本信息。 2.实验方法：外周血DNA相对端粒长度采用实时荧光定量PCR的方法进行分析。p16、RASSF1A和FHIT基因甲基化水平采用实时荧光定量甲基化特异性PCR的方法进行分析。 3.统计学分析：根据定量资料分布类型选择表示方法和统计学检验方法。定性资料比较采用用χ2检验。检验水准α=0.05。结果 1.结果显示煤焦沥青职业暴露人群端粒长度和对照组端粒长度分别为0.83(0.71-0.90)和1.13(0.72-1.45),两组比较差异有统计学意义(Z=-5.395,P0.001)。 2.职业暴露组和对照组p16基因启动子甲基化率分别为0.56(0.13-4.95)和0.37(0.06-4.23),两组差异无统计学意义(Z=-1.395,P=0.163)；职业暴露组和对照组FHIT启动子甲基化率分别为3.22(2.09-4.64)和2.95(1.45-5.88),两组差异无统计学意义(Z=-0.906,P=0.365)；职业暴露组和对照组RASSF1A基因启动子甲基化率分别为14.76(7.26-39.09)和19.34(3.82-50.95),两组差异无统计学意义(Z=-0.455,P=0.649)。结论煤焦沥青职业暴露可引起人外周血白细胞DNA端粒长度缩短；煤焦沥青职业暴露不能导致p16、RASSF1A和FHIT基因启动子甲基化率改变。
[Abstract]:Objective to investigate the promoter methylation and telomere damage of p16 FHIT and RASSF1A gene in occupational exposure population of coal tar pitch, and to explore the effect markers of occupational exposure of coal tar pitch. Object and method 1. Participants: one hundred and eighty cases of occupational contact with coal tar pitch were collected from 145 workers of a carbon factory in the central part of the university. All of them were examined in the physical examination department of the first affiliated hospital of our school. After informed consent, the subjects were collected peripheral blood, professional investigators to collect basic information. 2. 2. Methods: the relative telomere length of peripheral blood DNA was analyzed by real-time fluorescence quantitative PCR. The methylation level of p16 RASSF1A and FHIT gene was analyzed by real-time fluorescence quantitative methylation specific PCR. 3. Statistical analysis: according to the distribution type of quantitative data, the representation method and statistical test method were selected. The qualitative data were compared by 蠂 2 test. The test level is 伪 0. 05. Result 1. The results showed that telomere length was 0.83 (0.71-0.90) in occupational exposed population and 1.13 (0.72-1.45) in control group. The promoter methylation rate of p16 gene was 0.56 (0.13-4.95) and 0.37 (0.06-4.23) in occupational exposure group and control group, respectively, and there was no significant difference between the two groups (ZP0.163), and the methylation rate of FHIT promoter in occupational exposure group and control group was 3.22 (2.09-4.64) and 2.95 (1.45-5.88), respectively. The methylation rates of RASSF1A gene promoter in occupational exposure group and control group were 14.76 (7.26-39.09) and 19.34 (3.82-50.95), respectively. Conclusion Occupational exposure to coal tar pitch can shorten the length of DNA telomere in human peripheral blood leukocytes, while occupational exposure of coal tar pitch does not change the methylation rate of p16 RASSF1A and FHIT gene promoter.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R131

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