蜂王浆对溴代苯诱导小鼠氧化损伤的保护作用

发布时间：2018-08-30 18:21

【摘要】：研究不同取浆时间生产的蜂王浆(royal jelly,RJ)对溴代苯诱导小鼠氧化损伤的保护作用。以免移虫48 h生产的蜂王浆(48 h RJ)和72 h生产的蜂王浆(72 h RJ)为研究材料,灌胃给予昆明小鼠,蜂王浆分别设高剂量组9 g/(kg·d)(48 h RJ-H组、72 h RJ-H组)、中剂量组3 g/(kg·d)(48 h RJ-M组、72 h RJ-M组)和低剂量组1 g/(kg·d)(48 h RJ-L组、72 h RJ-L组),同时设一空白对照组。灌胃剂量为10 m L/kg,空白对照组小鼠则灌胃等量蒸馏水。连续灌胃45 d,然后给各组小鼠灌胃0.3 mg/kg溴代苯油溶液(灌胃剂量为10 m L/kg),22 h后测定小鼠的肝脏质量以及血清和肝脏组织中丙二醛(malondialdehyde,MDA)含量,超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、总抗氧化能力(total antioxidative capacity,T-AOC)活性。结果表明:预防性灌胃RJ对溴代苯攻击后小鼠的体质量、肝脏质量和肝脏系数无显著影响(P0.05);除48 h RJ-L组外,其他组小鼠血清中的MDA含量均显著低于空白对照组(P0.05),中、高剂量的RJ可显著升高小鼠血清中的SOD和GSH-Px活力,而所有灌胃RJ的小鼠血清T-AOC活力都显著高于空白对照组(P0.05);灌胃RJ也可显著降低小鼠肝脏组织中的MDA含量和GSH-Px、T-AOC活力(P0.05),除48 h RJ-L组外,其他组小鼠肝脏组织中SOD活力均显著高于空白对照组(P0.05)。48 h RJ和72 h RJ对溴代苯诱导小鼠氧化损伤均具有一定的保护作用,但两者存在一定差异。
[Abstract]:To study the protective effect of royal jelly (royal jelly,RJ) produced at different pulping times on the oxidative damage induced by brominated benzene in mice. The royal jelly (48 h RJ) and 72 h) produced in order to avoid moving insects for 48 h (72 h RJ) was given to Kunming mice by gavage. Royal jelly was divided into high dose group (9 g / (kg d) (48 h RJ-H group 72 h RJ-H group), middle dose group 3 g / (kg d) (48 h RJ-M group 72 h RJ-M group and low dose group 1 g / (kg d) (48 h RJ-L group 72 h RJ-L group). The dose was 10 mL / kg, while the control group was fed with distilled water. The liver quality and malondialdehyde (malondialdehyde,MDA) content in serum and liver tissue of mice were measured after oral administration of 0.3 mg/kg bromobenzene oil solution (10 m L/kg) for 22 h. Superoxide dismutase (superoxide dismutase,SOD), glutathione peroxidase (glutathione peroxidase,GSH-Px) and total antioxidant activity (total antioxidative capacity,T-AOC). The results showed that preventive oral administration of RJ had no significant effect on the body weight, liver quality and liver coefficient of mice after brominobenzene attack (P0.05), and the content of MDA in serum of other groups was significantly lower than that of the control group except for 48 h RJ-L group (P0.05). High dose of RJ could significantly increase the activity of SOD and GSH-Px in serum of mice. The serum T-AOC activity of all mice fed with RJ was significantly higher than that of the blank control group (P0.05), and the content of MDA and the activity of GSH-Px,T-AOC in the liver tissue of the mice were also significantly decreased by intragastric RJ administration (P0.05), except for 48 h RJ-L group. The activity of SOD in liver of other groups was significantly higher than that of control group (P0.05). 48 h RJ and 72 h RJ had protective effects on oxidative damage induced by brominated benzene in mice, but there were some differences between the two groups.
【作者单位】：江西农业大学蜜蜂研究所;江西中医药大学实验动物科技中心;
【基金】：国家现代农业(蜂)产业技术体系建设专项(CARS-45-kxj12) 赣鄱英才555工程资助项目(赣才字〔2013〕2号)
【分类号】：R151

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