80Hz低频电刺激早期治疗股二头肌急性拉伤实验研究

发布时间：2018-01-11 23:09

本文关键词：80Hz低频电刺激早期治疗股二头肌急性拉伤实验研究　出处：《中国运动医学杂志》2016年04期 　论文类型：期刊论文

【摘要】：目的:采用80 Hz低频电刺激早期治疗股二头肌急性拉伤,观察其对肌肉损伤部位结构重塑和功能的影响。方法:SD雄性大鼠36只,先按体重分层,再随机分为正常对照组和实验组,后者依据不同实验取材时间分为D0组、D7组、D7-80 Hz、D14组和D14-80 Hz组,每组6只。实验组大鼠实施麻醉,采用针电刺激使股二头肌强直收缩,同时膝关节角度从屈曲70°到完全伸直180°以角速度960°·s-1伸膝摆腿反向拉伸,建立股二头肌急性拉伤动物模型。造模后D7-80Hz组和D14-80 Hz组于动物造模后第5天开始给予80 Hz电刺激治疗(2次/日,30分钟/次,间隔4小时),其余组无特殊干预继续喂养。正常对照组、D0组、D7组、D7-80 Hz组、D14组和D14-80 Hz组分别在第0、0、7、7、14、14天进行在体力矩测试,观测膝关节力矩变化以及力矩角度关系。实验结束后处死动物,分离股二头肌,液氮固定、OCT包埋,组织切片、HE染色,光镜下观察股二头肌组织形态。结果:光镜观察可见肌肉拉伤当天肌纤维坏死,肌肉组织结构紊乱,肌纤维水肿变性,肌肉间隙大小不一。拉伤后第7天D7组观察可见,肌纤维虽然有所修复,但是形态不成熟,大小不一,排列不严整。经过80 Hz电刺激治疗,肌肉组织结构并没有明显改善。力矩测试D0组关节最大等长力矩值(0.246±0.026 Nm)下降明显,与其余各组比较均有显著性差异(P0.05);D7-80 Hz组(0.302±0.033 Nm)和D14-80 Hz组(0.334±0.060 Nm)关节最大等长力矩值分别与D7组和D14组比较均无显著性差异(P0.05)。正常对照组最优角度为130.00°±3.54°,经80 Hz电刺激治疗后D14-80 Hz组力矩角度关系曲线右偏移,关节最优角度为121.00°±4.18°,与D14组(120.00°±3.53°)比较并没有显著性差异(P0.05)。结论 :股二头肌急性拉伤后早期采用80Hz低强度电刺激治疗,2周时间内并不能有效地促进损伤肌肉结构重塑,也不能提高关节最大等长力矩以及优化关节力矩角度关系。
[Abstract]:Objective: to observe the effect of 80 Hz low frequency electric stimulation on the structural remodeling and function of biceps femoris muscle injury in early stage. Methods: 36 male SD rats were divided according to their body weight. Then they were randomly divided into normal control group and experimental group, the latter was divided into D0 group, D7 group and D14 group and D14-80 Hz group according to the time of sampling. Six rats in each group were anesthetized and the biceps femoris muscle was constricted by electroacupuncture. At the same time, the knee joint angle from flexion 70 掳to complete elongation 180 掳with 960 掳路s ~ (-1) extension knee swing leg reverse stretching. The animal model of acute strain injury of biceps femoris was established. The rats in D7-80Hz group and D14-80Hz group were treated with 80 Hz electric stimulation twice a day on the 5th day after the establishment of the model. The rest group was fed continuously without special intervention. The normal control group was treated with D0 group and the D7 group was treated with D7-80 Hz. D14 group and D14-80 Hz group were tested for in vivo torque on day 14 respectively. At the end of the experiment, the animals were killed, the biceps femoris were separated, the Oct was fixed with liquid nitrogen, and the tissue sections were stained with HE. Results: muscle fiber necrosis, muscle tissue structure disorder, muscle fiber edema and degeneration were observed under light microscope. The size of the muscle space was different. The D7 group was observed on the 7th day after the injury. Although the muscle fibers were repaired, they were immature in shape, different in size and not well arranged, and were treated with 80 Hz electrical stimulation. There was no significant improvement in muscle structure. The maximum isometric torque value of joint in D0 group was 0.246 卤0.026 Nm). Compared with other groups, there was a significant difference (P 0.05). D7-80 Hz (0.302 卤0.033 Nm) and D14-80 Hz (0.334 卤0.060 Nm). There was no significant difference in joint maximum equal length moment between D7 group and D14 group (P 0.05). The optimal angle of normal control group was 130.00 掳卤3.54 掳. After 80 Hz electric stimulation, the angle curve of D14-80 Hz group was shifted to the right, the optimal angle of joint was 121.00 掳卤4.18 掳. There was no significant difference between D14 group (120.00 掳卤3.53 掳) and D14 group (P0.050.Conclusion: 80 Hz low intensity electric stimulation was used in the early stage after acute strain injury of biceps femoris muscle. In 2 weeks, it can not effectively promote the remodeling of injured muscle structure, nor can it improve the maximum equal length moment of joint and optimize the angle relationship of joint torque.
【作者单位】：海南热带海洋学院体育与休闲学院;国家体育总局运动医学研究所;
【基金】：广东省自然科学基金(10451007601004461)
【分类号】：R873
【正文快照】： 肌肉拉伤是运动中最常见的损伤之一。目前电刺激在临床治疗肌肉损伤中广为应用,其有效性和安全性不断得到临床实践的证实。依据低频电刺激治疗的目的,电刺激频率变化范围可以很大,在临床治疗中10~100 Hz较为常用[1,2]。我们的研究证实急性拉伤后早期采用20 Hz低频电刺激能够产

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