DCM猝死与晚期DCM心肌Cx43表达的比较

发布时间：2018-01-16 15:15

本文关键词：DCM猝死与晚期DCM心肌Cx43表达的比较　出处：《中国法医学杂志》2016年01期 　论文类型：期刊论文

【摘要】：目的观察和探讨扩张型心肌病(DCM)猝死与晚期DCM心肌中心肌连接蛋白(connexin,Cx)43表达差异及其意义。方法收集13例DCM猝死者心脏(A组)和5例晚期DCM患者行心脏移植手术切除的心脏(B组),运用免疫组化染色和图像分析技术,检测心肌Cx43阳性表达产物的平均光密度(OD)值和面积(S)值,比较两组间和左右心室间的差异。结果 Cx43阳性表达在A组明显减少,分布不均;在B组表达清晰,主要位于闰盘处。A组与B组心肌Cx43的S值之间的差异有统计学意义(P0.01),而各组左、右心室肌间相比,差异无统计学意义(P0.05);心肌Cx43的OD值,在A组与B组之间,以及各组左、右心室肌间差异均无统计学意义(P0.05)。结论 DCM猝死心肌Cx43表达较晚期DCM心肌明显减少,这种变化可能与DCM患者因心律失常发生猝死有关。
[Abstract]:Objective to observe and investigate the relationship between sudden death of dilated cardiomyopathy (DCM) and cardiac junction protein connexin (connexin) in the late stage of DCM. Methods 13 patients with sudden death of DCM (group A) and 5 patients with late DCM underwent cardiac transplantation (group B). Immunohistochemical staining and image analysis were used to detect the average optical density and area of Cx43 positive expression products in myocardium. Results the positive expression of Cx43 in group A was significantly decreased and distributed unevenly. In group B, the expression was clear, mainly located at the intercalated disc. There was significant difference in S value of myocardial Cx43 between group A and group B, but there was a significant difference between the left and right ventricular muscle in each group. The difference was not statistically significant (P 0.05). The OD value of myocardial Cx43 was between group A and group B, and left in each group. Conclusion the expression of Cx43 in the myocardium of sudden death of DCM is significantly lower than that in late stage of DCM. This change may be associated with sudden death due to arrhythmia in patients with DCM.
【作者单位】：华中科技大学同济医学院法医病理学教研室;河南财经政法大学司法鉴定中心;武汉市公安局蔡甸分局;
【基金】：湖北省科技攻关计划项目(2007AA301B31-3) 武汉市科技攻关计划项目(201060626254)
【分类号】：D919.4
【正文快照】： 扩张型心肌病(dilated cardiomyopathy,DCM)是原发性心肌病的主要类型,也是引发猝死的常见心血管病之一。心肌缝隙连接蛋白(connexin,Cx),尤其是其中的心血管系统表达的连接蛋白43(Cx43)是心室肌细胞的主要连接蛋白,其表达及分布异常与心肌缺血、坏死及室性心律失常关系密切[1

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