血府逐瘀胶囊对模拟失重下骨骼肌萎缩的防护作用

发布时间：2018-01-28 12:50

本文关键词： 失重肌萎缩血府逐瘀胶囊 PIK/AKT　出处：《中华灾害救援医学》2016年11期 　论文类型：期刊论文

【摘要】：目的探讨血府逐瘀胶囊在模拟失重状态下对骨骼肌萎缩的防护作用。方法建立尾部悬吊大鼠模拟失重模型,30只同源同窝雄性SD大鼠随机分为对照组、尾吊组和血府逐瘀组(尾吊+血府逐瘀胶囊),每组10只。对照组正常饲养,尾吊组和血府逐瘀组均尾吊30 d。对照组及尾吊组单给予纯溶媒,血府逐瘀组给予血府逐瘀胶囊,剂量为25 mg/(kg·d),每组8:00灌胃,1次/d。(1)测量比目鱼肌湿重体质量比和横截面积。(2)应用免疫蛋白质印迹法(Western-blot)技术检测PI3K/AKT通路相关蛋白及下游分子GRP78、BAX、Cyto-c蛋白表达水平。(3)应用caspase-3活性检测试剂盒检测caspase-3活性。结果 30只大鼠全部进入结果分析,无脱失。(1)与对照组相比,尾吊组比目鱼肌平均湿重体质量比下降了56.39%;血府逐瘀组平均湿重体质量比较单纯尾吊组增加了14.49%,且差异均有统计学意义(P0.05)。(2)尾吊30 d显著抑制了PI3K/AKT信号通路中PI3K、p-AKT的蛋白表达水平,与对照组比较,血府逐瘀组干预后提高了PI3K、p-AKT的表达,其中对关键蛋白p-AKT的表达影响最大,与尾吊组比较,差异有统计学意义(P0.05)。(3)尾吊30 d比目鱼肌caspase-3酶活性显著增强,血府逐瘀组caspase-3酶活性较尾吊组显著降低,差异有统计学意义(P0.05)。结论 (1)尾吊模拟失重可通过抑制PI3K/AKT通路相关蛋白表达水平,增强caspase-3酶活性,导致比目鱼肌萎缩;(2)血府逐瘀胶囊可能通过提高PI3K/AKT通路相关蛋白表达水平,对失重性肌萎缩起到保护作用。
[Abstract]:Objective to investigate the protective effect of Xuefu Zhuyu capsule on skeletal muscle atrophy in simulated weightlessness. Tail suspension group and Xuefu Zhuyu group (10 rats in each group). The control group was fed normally, the tail suspension group and Xuefu Zhuyu group were all treated with tail suspension for 30 days. The control group and tail suspension group were given pure solvent only. Xuefu Zhuyu group was given Xuefu Zhuyu capsule at the dose of 25 mg/(kg 路dl, each group was gavaged at 8:00. Measurement of wet body mass ratio and cross sectional area of soleus muscle. PI3K/AKT pathway related proteins and downstream GRP78 were detected by RT-PCR. Caspase-3 activity assay kit was used to detect caspase-3 activity. Results all 30 rats entered the result analysis. Compared with the control group, the average wet weight ratio of soleus muscle in tail suspension group was 56.39; The average body weight of Xuefu Zhuyu group was 14.49% higher than that of single tail suspension group. And the difference was statistically significant (P 0.05A) tail suspension significantly inhibited the protein expression level of PI3Knp-AKT in the PI3K/AKT signaling pathway for 30 days, compared with the control group. The expression of PI3Knp-AKT was increased in Xuefu Zhuyu group, and the expression of the key protein p-AKT was the most significant, compared with that of tail suspension group. The activity of caspase-3 in soleus muscle was significantly increased after 30 days of tail suspension. The activity of caspase-3 in Xuefu Zhuyu group was significantly lower than that in tail suspension group. Conclusion simulated weightlessness by tail suspension can enhance the activity of caspase-3 by inhibiting the expression of protein associated with PI3K/AKT pathway. Cause soleus muscle atrophy; Xuefu Zhuyu capsule may protect weightlessness muscle atrophy by increasing the expression level of PI3K/AKT pathway related protein.
【作者单位】：武警总医院神经内科;中国航天员科研训练中心;
【基金】：武警总医院院内课题三类(WZ2012041)
【分类号】：R852.22
【正文快照】： 航天失重环境下,宇航员的身体机能会发生一系列病理变化,失重或模拟失重可引起骨骼肌(特别是抗重力肌)萎缩,进而导致肌肉收缩力降低、易疲劳性增加,严重限制了人类航天事业的发展[1]。失重性肌萎缩的发生机制目前仍不清楚,失重条件下骨骼肌的萎缩是一个多因素相互作用的结果,

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