糖有氧供能条件下不同训练周期的游泳训练后大鼠肾脏功能的恢复规律研究

发布时间：2018-02-08 09:36

本文关键词： 周期训练糖有氧肾脏恢复规律　出处：《成都体育学院》2012年硕士论文　论文类型：学位论文

【摘要】：目的：在游泳训练中内脏器官机能的好坏直接影响到运动水平的高低，而在游泳训练后运动能力的恢复及提高也直接受制于内脏器官机能恢复水平。由于无氧供能条件下游泳运动后肾脏的恢复规律的研究已有报道。本文模拟在糖有氧供能条件下，将一周作为一个训练单元并安排进行单、双、三周期的游泳训练后，研究经游泳训练后的大鼠肾脏功能在72小时恢复过程中的恢复规律，为糖有氧供能条件下的间歇性游泳训练的最佳训练周期/周提供实验依据。方法：将健康雄性SD大鼠132只，采用随机数字法分成空白对照组(C)和训练组(T)两大组，对照组6只；单周期/周训练组（T1组，n=42）、双周期/周训练组（T2组，n=42）、三周期/周训练组（T3组，n=42）。其中T1组、T2组、T3组根据训练结束后处死动物的时间各自分为以下组别：A(训练结束即刻组)、B(训练结束12h组)、C(训练结束24h组)、D(训练结束36h组)、E(训练结束48h组)、F(训练结束60h组)、G(训练结束72h组)。按照不同运动量和训练周期对大鼠进行游泳训练，，一周训练结束后，C组在安静状态下，T1、T2、T3各组在运动后即刻、12h、24h、36h、48h、60h和72h处死大鼠分批取材肾脏组织，取血，分离血清，测定肌酐（CRE）和肾脏组织超氧化物歧化酶（SOD）、（丙二醛(MDA）、Ca~(2+)-ATP酶等指标，对肾脏组织固定、切片作HE染色，光镜观察。结果：1.整个实验期对照组体重呈正性增长，周期训练组实验前后体重无明显变化(P0.05)。2.单周期训练结束后即刻大鼠血液中肌酐CRE含量（98.06±4.76）升高(P0.05)，具有显著性意义；肾脏组织中SOD含量单周期训练结束后即刻略有升高，随后各时相虽有变化，但不具有统计学意义(P0.05)；肾脏组织中MDA含量除即刻（23.99±2.68）显著性升高(P0.05)其余时相变化无统计学意义；Ca~(2+)-ATPase含量在即刻（16.87±3.22）和12h（15.79±3.99）表现为显著性降低(P0.05)。3.双周期训练结束后肾脏组织Ca~(2+)-ATPase含量各时相变化明显，在12h（19.76±1.35）(P0.05)、24h(23.02±0.73)(P0.01)和36h(20.49±1.79)(P0.05)变现为显著性降低和非常显著性回升；肾脏组织内SOD水平在即刻（23.13±3.41）表现出显著性降低(P0.05)，24h（24.77±3.19）和36h（25.98±3.89）显著性升高(P0.05)，60h恢复至正常水平；即刻大鼠血液中肌酐（CRE）（134.1±8.27）水平非常显著性升高(P0.01)；4.三周期训练后即刻大鼠血液中肌酐含量(96.98±2.65)显著性升高(P0.05)，24h（99.73±2.26）和36h（95.7±2.31）均表现显著性升高和回落(P0.05)，72h时恢复至正常水平；大鼠肾脏组织内SOD水平即刻（27.66±1.48）表现为显著性降低(P0.05)；MDA各时相与安静对照组（15.14±1.81）比较，有上升趋势，但都不具有统计学意义，至72h恢复至正常水平；肾脏组织内Ca~(2+)-ATPase水平在12h(15.31±2)、24h(15.15±2.33)和48h(16.04±1.7)均表现为显著性下降。结论：1.单周期/周训练后肾脏功能恢复的最佳时间点(区间)不具有统计学意义，单周期/周训练训练方法对于提高机体机能没有显著性意义；2.双周期/周训练后，肾脏功能恢复的最佳时间点(区间)为48h至60h左右,有显著性意义；3.三周期/周训练后肾脏功能恢复的最佳时间点(区间)为60h至72h左右,有显著性意义。
[Abstract]:Objective : To study the effect of the function of visceral organs directly on the level of internal organs during swimming training , while the recovery and improvement of the exercise ability after swimming training were directly influenced by the level of internal organ dysfunction . After swimming training , the recovery of the kidney function of rats after swimming training was studied in 72 hours after swimming training . The experimental basis was provided for the optimal training period / week of intermittent swimming training under the condition of aerobic energy supply . Methods : 132 healthy male SD rats were divided into two groups : blank control group ( C ) and training group ( T ) . The control group was 6 rats ; single cycle / week training group ( T1 group , n = 42 ) , two cycle / week training group ( T2 group , n = 42 ) , three cycle / week training group ( T3 group , n = 42 ) . The rats were divided into three groups : group A ( immediate group ) , group B ( training end 12h group ) , C ( training end 24h group ) , D ( training end 36h group ) , E ( training end 48h group ) , F ( training end 60h group ) , G ( training end 72h group ) , group C ( training end 60h group ) and G ( training end 72h group ) . Results : 1 . There was a significant decrease in the level of serum creatinine ( 98.06 卤 4.76 ) in renal tissue ( P0.05 ) . The level of SOD in renal tissue was significantly lower than that in the control group ( P0.05 ) . Conclusion : 1 . The optimal time point ( interval ) of renal function recovery after single cycle / week training is not significant . The optimal time point ( interval ) of renal function recovery after double cycle / week training is about 48h to 60h .

【学位授予单位】：成都体育学院
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：G804.2

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