放射性口干症动物模型建立及颌下腺放射性组织损伤的生物学效应

发布时间：2018-02-08 12:09

  本文关键词： 口腔干燥 放射性 模型 动物 颌下腺 组织工程 组织构建 放射性口干症 动物模型 直线加速器 广东省自然科学基金　出处：《中国组织工程研究》2017年32期 　论文类型：期刊论文

【摘要】：背景:目前对于唾液腺的放射性损伤机制还未明确,治疗和防护措施也处于探索阶段。目的:建立放射性口干症大鼠模型,并观察18 Gy电子线照射后大鼠颌下腺组织损伤情况及唾液成分变化。方法:通过随机对照实验,将115只Wistar大鼠随机分成照射组和假照射组,假照射组只麻醉不照射,照射组麻醉后使用直线加速器一次性照射18 Gy。将大鼠颌下腺部位暴露于照射区,制备放射性口干症模型。记录造模后21 d内饮水量;分1,3,7,14,21,28,35,42 d 8个时间点收集唾液、摘取颌下腺腺体,测量唾液量、颌下腺指数,对颌下腺切片行苏木精-伊红染色观察组织形态学变化。结果与结论:(1)放射后1-21 d照射组大鼠每天平均饮水量为(6.42±1.91)m L,假照射组为(4.82±1.20)m L,差异有显著性意义(P0.05);(2)在放射后42 d内照射组唾液量均少于假照射组,其中照射组大鼠照射后第7天唾液量降至最低;7,21,28,42 d比较差异有显著性意义(P0.05);(3)1 d及21-42 d,照射组颌下腺指数均小于假照射组(P0.05);(4)苏木精-伊红病理结果显示,照射组大鼠颌下腺炎性浸润、腺体萎缩、核固缩等损伤情况呈渐进性加重,放射后42 d损伤最为严重;(5)综上,Wistar大鼠接受直线加速器电子线18 Gy照射后,放射性口干症模型成功建立,直观表现为饮水量增加,唾液量减少,病理切片显示颌下腺病理损伤程度在42 d内随时间的增加呈进行性加重。
[Abstract]:Background: the mechanism of radioactive damage of salivary glands is not clear, and the treatment and protection measures are still in the exploring stage. Objective: to establish a rat model of radiation-induced xerostomia. The changes of submandibular gland tissue and salivary composition in rats after 18 Gy electron irradiation were observed. Methods: 115 Wistar rats were randomly divided into irradiation group and false irradiation group. After anesthesia, the rats in the irradiation group were exposed to the irradiation area with a linear accelerator for 18 Gy.The rats were exposed to the irradiation area to make a model of radioorrhagia. The drinking water was recorded within 21 days after the model was made, and saliva was collected at 8 time points of 1: 3, 71421, 2828, 3542 days, respectively, after the exposure of the submandibular gland of the rats to the irradiation area. The submandibular gland was removed, salivary volume was measured, and submandibular gland index was measured. The histological changes were observed by hematoxylin-eosin staining on submandibular gland sections. Results and conclusions the average daily drinking water of rats in 1-21 d radiation group was 6. 42 卤1. 91 mL, and that in sham-irradiated group was 4. 82 卤1. 20 mL. the difference was significant (P 0. 05 2). The amount of saliva in the irradiation group was less than that in the false irradiation group within 42 days after irradiation. The salivary volume of rats in the irradiation group decreased to the lowest level on the 7th day after irradiation. There was a significant difference in saliva volume between the two groups on the 7th day after irradiation. There was a significant difference between the two groups on the 1st day and 21-42 d after irradiation. The index of submandibular gland in the irradiation group was lower than that in the sham irradiation group (P 0.05). The pathological results of hematoxylin and eosin showed that the submandibular gland index in the irradiated group was lower than that in the sham irradiation group. The inflammatory infiltration of submandibular gland, atrophy of glands and nuclear pyknosis were gradually aggravated in the irradiation group, and the damage was the most serious at 42 days after irradiation. The model of xerostomia was established successfully. The visual manifestation was that the quantity of drinking water increased and the amount of saliva decreased. The pathological section showed that the degree of pathological injury of submandibular gland increased progressively with the increase of time within 42 days.
【作者单位】： 广州中医药大学护理学院;广东省中医院大学城放疗科;
【基金】：广东省自然科学基金项目(2014A030313405)~~
【分类号】：R-332;R730.55
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