不同训练强度影响骨腱结合部损伤的机理研究

发布时间：2018-02-08 17:09

  本文关键词： 骨腱结合部 损伤 细胞外基质 机制 循环载荷强度　出处：《北京体育大学》2012年博士论文　论文类型：学位论文

【摘要】：研究目的：骨腱结合部损伤是一种困扰运动训练和人们日常生活的常见损伤,过度负荷是主要原因,其病理变化过程还不十分清楚。基质是肌腱载荷的主要承担者,在损伤的修复中有重要的作用。本文以不同强度的循环载荷作为预手段,对骨腱结合部损伤的机理进行研究探讨。揭示骨腱结合部损伤发生发展过程中的病理变化,以及不同载荷量引起的各种指标相应的变化规律,确定干引起损伤的载荷量。 研究方法：48只新西兰大白兔随机分为6组,每组8只。其中一组为对照组,其余5组为实验组。采用循环载荷的刺激方式,强直收缩力的30%为100%的刺激负荷强度组,其它各组负荷强度依次采取此刺激强度的20%、40%、60%、80%,进行循环载荷刺激2小时/天,3天/周。4周后宰杀取材进行相应的¨E染色和免疫组织化学染色。 研究结果：1.各负荷组骨腱结合部组织形态发生了改变。高运动负荷组,肌腱细胞密度发生显著性增加。纤维软骨带厚度无显著性差异。2.Ⅲ型胶原纤维发生显著性增加,从60%载荷强度开始与CON组、L20、L40载荷组出现显著性差异。Aggrecan免疫组化结果显示无显著差异。Tenascin-C表达出现显著性改变,60%载荷刺激强度时表达下降,L80、L100组比CON组、L20、L60载荷组显著性升高。TGF随负荷刺激强度的增加而增加,L20、L40载荷刺激强度与CON组比较出现显著性差异,L60、L80、L100载荷刺激强度组与CON组出现非常显著性差异。3.各指标相关结果显示,细胞密度与Ⅲ型胶原纤维、TGF具有非常显著性相关。TGF与型胶原纤维和TN有显著性相关。 研究结论：1不同载荷强度作用于骨腱结合部4周,L60组、L80组、L100组出现损伤病理变化,说明这种低强度循环载荷叮以引起骨腱结合部损伤的发生2运动强度的不同导致骨腱结合部组织发生了相应的结构和成分改变。说明了骨腱结合部损伤程度与运动强度有关。3在损伤的病理变化中,Ⅲ型胶原纤维、Tenasin-C发生了显著性增加,说明骨腱结合部损伤的发生中,细胞外基质具有重要作用。4不同载荷强度作用于骨腱结合部4周,TGF表达发生了显著性增加,并且与细胞密度、Ⅲ型胶原纤维、TNC有显著性相关,说明TGF在骨腱结合部损伤修复过程中是重要的调节因素。
[Abstract]:Objective: bone tendon joint injury is a common injury that puzzles sports training and people's daily life. Overloading is the main cause and the pathological process is not very clear. The matrix is the main load bearer of tendon. In this paper, the mechanism of bone tendon joint injury is studied by using different cyclic load as preprocessing method, and the pathological changes during the development of bone tendon joint injury are revealed. According to the change law of various indexes caused by different load quantity, the load quantity caused by dry damage is determined. Methods Forty-eight New Zealand white rabbits were randomly divided into 6 groups with 8 rabbits in each group. One group was the control group and the other five groups were the experimental group. The other groups took 20% of the stimulus intensity in turn. The rats were subjected to cyclic load stimulation for 2 hours / day / 3 days / week for 4 weeks and then slaughtered for E staining and immunohistochemical staining. Results 1. The tissue morphology of bone tendon junctions in each load group was changed. In the high exercise load group, the density of tendon cells increased significantly, and the thickness of fibrous chondrocytes showed no significant difference .2. type 鈪，

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