多功能铁铋复合纳米粒对胶质母细胞瘤的放疗增敏作用

发布时间：2018-02-11 14:30

本文关键词：透明质酸纳米复合物胶质母细胞瘤组织工程生物材料纳米材料铁铋复合纳米材料水热聚醇法放疗增敏人胶质母细胞瘤细胞生物相容性杀伤肿瘤细胞增殖率　出处：《中国组织工程研究》2017年18期 　论文类型：期刊论文

【摘要】：背景:透明质酸功能化的铁铋复合纳米颗粒是一种有效MRI对比剂,同时又可作为放疗增敏剂。目的:制备透明质酸功能化的铁铋复合纳米颗粒,观察其对胶质母细胞瘤U87MG的放疗增敏作用。方法:通过水热聚醇法制备透明质酸功能化的铁铋复合纳米颗粒HA-Bi IOPs。(1)细胞毒性实验:取对数生长期的人胶质母细胞瘤细胞株U87MG与大鼠血管平滑肌细胞,均以含0,12.5,25,50,100,200,400 mg/L HA-Bi IOPs的培养液培养48 h,计算细胞增殖率;(2)组织相容性实验:在ICR小鼠尾静脉注射HA-Bi IOPs溶液,观察小鼠脏器病理变化;(3)细胞吞噬实验:将HA-Bi IOPs与U87MG细胞共培养6 h,普鲁士蓝观察纳米颗粒是否被细胞吞噬;(4)放疗增敏实验:取对数生长期的U87MG细胞,分组培养:对照组以培养液培养;放疗组分别给予0,3,6,9 Gy的X射线照射;HA-Bi IOPs组分别加入含0,12.5,25,50,100,200,400 mg/L HA-Bi IOPs的培养液;联合组,先加入含0,12.5,25,50,100,200,400 mg/L HA-Bi IOPs的培养液,再给予0,3,6,9 Gy的X射线照射。24 h后,检测细胞增殖率及克隆形成率。结果与结论:(1)不同质量浓度的HA-BiI OPs对血管平滑肌细胞和U87MG细胞增殖无明显影响;(2)尾静脉注射HA-BiI OPs溶液后,小鼠脏器未发生病理变化;(3)共培养6 h后,HA-BiI OPs纳米颗粒可被U87MG细胞吞噬;(4)U87MG细胞增殖率与HA-BiI OPs质量浓度(0-200 mg/L)和放射剂量(0-9 Gy)有明显的线性负相关,尤其是在6 Gy X射线照射下200 mg/L HA-BiI OPs对细胞增殖率下降至(41±7)%。选用100 mg/L HA-BiI OPs、6 Gy X射线照射进行克隆形成实验,联合组U87MG细胞增殖率明显低于空白对照组、放疗组(P0.05);(5)结果表明,透明质酸功能化的铁铋复合纳米颗粒对胶质母细胞瘤具有显著的放疗增敏作用。
[Abstract]:Background: Iron-bismuth composite nanoparticles with hyaluronic acid functionalization are effective MRI contrast agents and can also be used as radiosensitizers. Objective: to prepare iron-bismuth composite nanoparticles with hyaluronic acid functionalization. To observe the radiosensitizing effect of U87MG on glioblastoma. Methods: the cytotoxicity of hyaluronic acid-functionalized iron-bismuth composite nanoparticles HA-Bi IOPs.-1 was prepared by hydrothermal polyol method: the logarithmic phase of human glioblastoma was taken. Cell line U87MG and rat vascular smooth muscle cells, All of them were cultured for 48 hours in a culture medium containing 0 ~ 12.5T 2550U ~ (100) ~ (#number0#) mg/L HA-Bi IOPs. The cell proliferation rate was calculated by ~ (2)) histocompatibility experiment: HA-Bi IOPs solution was injected into caudal vein of ICR mice. HA-Bi IOPs and U87MG cells were co-cultured for 6 h. Prussian blue was used to observe whether the nanoparticles were phagocytized by the cells. The radiosensitization test: U87MG cells in logarithmic phase were taken. Group culture: the control group was cultured in culture medium; the radiotherapy group was treated with X ray irradiation of 0 ~ 3g ~ 6g ~ 9 Gy, respectively, and the HA-Bi IOPs group was added with a culture medium containing 0 ~ 12.5g ~ 2550g ~ 100g ~ (100) mg/L HA-Bi IOPs, respectively; the combined group was treated with a culture medium containing 0 ~ (12.5U) ~ 2550U ~ (50) ~ (100) mg/L HA-Bi IOPs, respectively. The cell proliferation rate and clone formation rate were measured after irradiation of 0 ~ 3G ~ 69 Gy for 24 h. Results and conclusion HA-BiI OPs of different concentration had no significant effect on the proliferation of vascular smooth muscle cells and U87MG cells. (2) HA-BiI OPs solution was injected into tail vein. There was a significant linear negative correlation between the proliferation rate of HA-BiI OPs nanoparticles phagocytized by U87MG cells and the concentration of HA-BiI OPs 0-200 mg / L and radiation dose of 0-9 Gy. The proliferation of U87MG cells in the combined group was significantly lower than that in the blank control group, and the proliferation rate of U87MG cells in the combined group was significantly lower than that in the control group. The results showed that the proliferation rate of U87MG cells in the combined group was significantly lower than that in the control group (P 0.05), and the proliferation rate of U87MG cells in the combined group was significantly lower than that in the control group. Hyaluronic acid-functionalized iron-bismuth composite nanoparticles have a significant radiosensitization effect on glioblastoma.
【作者单位】：江苏大学附属医院神经内科;江苏大学医学院;江苏大学附属医院医学影像科;
【基金】：江苏省“333工程”科研项目(BRA2014173) 江苏省“六大人才高峰”科研项目(WSN-038)~~
【分类号】：R730.55;R739.41

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