过敏性和冠心病猝死者心肌组织中肥大细胞类胰蛋白酶、脑利钠肽的表达

发布时间：2018-02-17 00:43

本文关键词： 法医病理学猝死过敏反应冠状动脉硬化肥大细胞类胰蛋白酶类利钠肽脑　出处：《法医学杂志》2016年03期 　论文类型：期刊论文

【摘要】：目的探讨肥大细胞类胰蛋白酶、脑利钠肽(brain natriuretic peptide,BNP)在过敏性猝死和冠心病猝死鉴别诊断中的意义。方法选取山西医科大学法医病理学教研室2010—2015年尸检案例心肌标本共30例,分为颅脑损伤致死组、过敏性猝死组、冠心病猝死组,每组各10例。采用免疫荧光染色和Western印迹法分析各组心肌组织肥大细胞类胰蛋白酶和BNP的表达。结果过敏性猝死组、冠心病猝死组心肌组织内肥大细胞类胰蛋白酶免疫荧光染色均出现阳性染色;三组间两两比较,表达差异均具有统计学意义(P0.05)。冠心病猝死组心肌组织内BNP的表达量高于过敏性猝死组、颅脑损伤致死组(P0.05),过敏性猝死组与颅脑损伤致死组之间差异无统计学意义(P0.05)。结论联合检测心肌组织内肥大细胞类胰蛋白酶、BNP有望为过敏性猝死和冠心病猝死的法医学鉴别诊断提供帮助。
[Abstract]:Objective to investigate the significance of mast cell trypsin and brain natriuretic peptide (BNP) in the differential diagnosis of sudden allergic death and sudden death of coronary heart disease. Methods 30 autopsy cases were collected from the Department of Forensic Pathology of Shanxi Medical University from 2010 to 2015. There were 10 cases in each group. The expression of trypsin and BNP in cardiac myocytes was analyzed by immunofluorescence staining and Western blotting. Positive staining of trypsin like trypsin was found in myocardium of sudden coronary heart death group. The expression of BNP in myocardial tissue of sudden coronary heart death group was higher than that of allergic sudden death group. There was no significant difference between the sudden allergic death group and the craniocerebral injury death group. Conclusion the combined detection of mast cell trypsin like trypsin in myocardial tissue is expected to be the method of sudden allergic death and sudden death of coronary heart disease. Medical differential diagnosis is helpful.
【作者单位】：山西医科大学法医学院法医病理教研室;盂县公安局;
【基金】：国家自然科学基金面上资助项目(81172905) 山西省科技攻关资助项目(20140313015-7)
【分类号】：D919

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