低氧复合运动抑制低氧诱导的骨骼肌NLRP3炎性小体活化

发布时间：2018-03-02 10:54

本文关键词： 低氧复合运动骨骼肌 NLRP炎性小体 Nrf ROS　出处：《中国运动医学杂志》2017年10期 　论文类型：期刊论文

【摘要】：目的:观察低氧复合运动是否能抑制低氧诱导的NLRP3炎性小体活化,并探讨Nrf2在其间的生物角色。方法:32只雄性SD大鼠随机分为常氧组(NC)、常氧运动组(NT)、低氧组(HC)和低氧复合运动组(HT),每组8只。低氧干预为常压低氧帐篷,11.3%氧浓度持续暴露4周。运动干预为跑台训练(5°,15 m/min),60 min/d,5 d/周,共4周。各组大鼠末次低氧后即刻处死,分离双侧股四头肌,单侧肌肉采用差速离心法提取线粒体,二氯荧光素法检测线粒体ROS生成速率,高效液相色谱法检测线粒体DNA中8-oxod G含量,ELISA法检测骨骼肌IL-1β含量,比色法检测骨骼肌Caspase-1活性,Western blot法检测骨骼肌NLRP3、ASC、Nrf2和NQO1蛋白表达。结果:HC组线粒体ROS生成速率和线粒体DNA中8-oxod G含量显著高于NC组(P0.01),骨骼肌Caspase-1活性和IL-1β含量显著高于NC组(P0.01),NLRP3和ASC蛋白表达显著高于NC组(P0.01),Nrf2和NQO1蛋白表达显著低于NC组(P0.05,P0.01)。HT组线粒体ROS生成速率和线粒体DNA中8-oxod G含量显著低于HC组(P0.01),骨骼肌Caspase-1活性和IL-1β含量显著低于HC组(P0.05,P0.01),NLRP3和ASC蛋白表达显著低于HC组(P0.05,P0.01),Nrf2和NQO1蛋白表达显著高于HC组(P0.01)。结论:低氧复合运动可上调Nrf2表达,通过减少ROS产生和促进抗氧化酶表达,抑制低氧诱导的NLRP3炎性小体活化。
[Abstract]:Aim: to observe whether hypoxia combined exercise can inhibit the activation of NLRP3 inflammatory bodies induced by hypoxia. Methods 32 male Sprague-Dawley rats were randomly divided into normal oxygen group, normoxic exercise group and hypoxic exercise group, 8 rats in each group. After 4 weeks of continuous exposure, the exercise intervention was as follows: 5 掳/ min of 15 m / min ~ (-1) ~ 60 min / d ~ (-1) / d ~ (5) d / week for the treadmill training. The rats in each group were killed immediately after the last hypoxia for 4 weeks. The quadriceps femoris muscles were isolated. The mitochondria were extracted by differential centrifugation and the rate of mitochondrial ROS production was detected by dichlorofluorescein method. Determination of 8-oxod G content in Mitochondrial DNA by High performance liquid Chromatography and Elisa method for Detection of IL-1 尾 in skeletal muscle. The Caspase-1 activity of skeletal muscle was detected by colorimetry and the expression of NLRP3ASCNrf2 and NQO1 protein was detected by Western blot. Results the ROS production rate of mitochondria and the content of 8-oxod G in mitochondrial DNA were significantly higher than those in NC group. The activity of Caspase-1 and the content of IL-1 尾 in skeletal muscle were significantly higher than those in NC group. The expression of NLRP3 and ASC protein in NC group was significantly higher than that in NC group, and the expression of Nrf2 and NQO1 protein in NC group was significantly lower than that in P0.05 and P0.01- HT group. The production rate of mitochondrial ROS and the content of 8-oxod G in mitochondrial DNA in NC group were significantly lower than those in HC group. The Caspase-1 activity and IL-1 尾 content in skeletal muscle were significantly lower than those in HC group. The expression of NLRP3 and ASC protein was significantly lower in group P0.05, P0.01 than that in group HC, and the expression of Nrf2 and NQO1 was significantly higher than that in group HC. Conclusion: hypoxia combined exercise can upregulate the expression of Nrf2. By reducing the production of ROS and promoting the expression of antioxidant enzymes, the activation of NLRP3 inflammatory corpuscles induced by hypoxia was inhibited.
【作者单位】：天津体育学院天津市运动生理学与运动医学重点实验室;武警后勤学院军事训练医学教研室;
【基金】：国家自然科学基金(31571224,81370454,31110103919) 天津市自然科学基金(14JCZDJC36600) 武警后勤学院研究基金(WHRGJ201623)
【分类号】：R87

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