低氧训练对大鼠小肠黏膜屏障功能的影响及其机制

发布时间：2018-03-02 20:05

本文选题：低氧　切入点：大强度运动　出处：《中国运动医学杂志》2017年04期 　论文类型：期刊论文

【摘要】：目的:探讨低氧训练对大鼠小肠黏膜屏障功能的作用和机制。方法:5周龄雄性SD大鼠80只,随机分为常氧对照组(C组,n=20)、常氧训练组(E组,n=20)、低氧对照组(HC组,n=20)、低氧训练组(HE组,n=20),通过人工低氧和游泳训练模拟高原训练,分别观察2周、6周后大鼠小肠黏膜的组织结构和超微结构,检测血浆中二胺氧化酶(DAO)、D-乳酸(D-La)以及肠组织中肿瘤坏死因子(TNF-α)、核因子-κB(NF-κB)的含量和小肠黏膜中紧密连接蛋白occludin mRNA的表达量。结果:(1)实验6周后,透射电镜观察显示,与常氧对照组相比,常氧训练组大鼠小肠微绒毛较稀疏,且排列不整齐,黏膜上皮细胞之间的间隙变宽,线粒体数量明显减少,嵴模糊;低氧对照组小肠微绒毛长度显著缩短,排列较为紊乱,黏膜上皮细胞之间的间隙变宽,少量线粒体变性;低氧训练组小肠微绒毛数量明显减少,长度明显缩短,肠绒毛出现倒伏,黏膜上皮细胞之间的间隙变宽,细胞结构不清晰,线粒体变性,部分线粒体嵴模糊、甚至消失。(2)2周后,运动训练可显著减少小肠绒毛数量(P0.01),显著提高血浆DAO、D-La(P0.05)以及小肠组织中NF-κB含量(P0.01);而低氧暴露可显著降低小肠组织occludin mRNA表达(P0.01),显著提高血浆DAO、D-La(P0.05)以及小肠组织TNF-α(P0.05)、NF-κB(P0.01)含量;低氧暴露联合运动训练对进一步减少小肠绒毛数量、长度和occludin mRNA表达,升高血浆DAO、D-La以及小肠组织TNF-α、NF-κB含量均无显著的交互作用(P0.05)。(3)6周后,运动训练及低氧暴露均显著降低小肠绒毛数量(P0.01),增加血浆DAO、D-La含量(P0.01)以及小肠组织中TNF-α(P0.01,P0.05)和NF-κB含量(P0.01),显著降低小肠组织中occludin mRNA的表达(P0.05);且低氧联合运动训练对进一步减少小肠绒毛数量、长度(P0.01,P0.05),增加血浆中DAO、D-La含量(P0.01)具有显著的交互作用。结论:(1)大强度运动训练以及低氧暴露均能导致肠道黏膜屏障功能受损,其损害程度与训练及低氧暴露时间有关。(2)低氧暴露以及大强度训练可能是通过增加小肠内TNF-α、NF-κB的含量从而降低紧密连接蛋白occludin的表达,最终导致小肠通透性增加,肠道黏膜屏障功能受损。
[Abstract]:Objective: to investigate the effect and mechanism of hypoxic training on intestinal mucosal barrier function in rats. They were randomly divided into normal oxygen control group (C), normoxic training group (E), hypoxia control group (HC) and hypoxia training group (HE). Altitude training was simulated by artificial hypoxia and swimming training. The tissue structure and ultrastructure of intestinal mucosa were observed in rats after 2 weeks and 6 weeks, respectively. The contents of TNF- 伪 and NF- 魏-魏 NF- 魏 B in plasma and the expression of tight junction protein occludin mRNA in intestinal mucosa were measured. Compared with the normoxic control group, the intestinal microvilli in the normoxic training group were sparse and irregular, the gap between the mucosal epithelial cells widened, the number of mitochondria decreased significantly and the ridge was blurred, the length of the intestinal microvilli in the hypoxic training group was significantly shorter than that in the hypoxic control group. In the hypoxic training group, the number and length of intestinal microvilli decreased significantly, the intestinal villi collapsed, and the gap between mucosal epithelial cells became wider. The cell structure was not clear, mitochondria degenerated, some mitochondria crest were blurred, and even disappeared 2 weeks later, Exercise training significantly decreased the number of villi and increased the content of NF- 魏 B in plasma DAO- D-La-P0.05) and small intestine tissues, while hypoxia exposure significantly decreased the expression of occludin mRNA in small intestine, significantly increased the content of plasma DAOD-LaP0.05) and the content of TNF- 伪 -P0.05- 魏 BP0.01 in small intestine. There was no significant interaction between hypoxia exposure and exercise training for further reducing the number of villi, length and occludin mRNA expression, increasing plasma DAOD-La and the content of TNF- 伪 -NF- 魏 B in small intestine. After 6 weeks, there was no significant interaction between hypoxia exposure and exercise training. Exercise training and hypoxic exposure significantly decreased the number of villi of small intestine and increased the content of DAOD-La in plasma and the contents of TNF- 伪 -P0.01P0.05) and NF- 魏 B in small intestine tissues, which significantly decreased the expression of occludin mRNA in intestinal tissues (P0.05). Reduce the number of villi, The increase of DAOOD-La content in plasma (P0.01) has a significant interaction. Conclusion: high intensity exercise training and hypoxia exposure can all result in impaired intestinal mucosal barrier function. The degree of injury is related to training and hypoxic exposure time.) hypoxia exposure and high intensity training may decrease the expression of tight junction protein occludin by increasing the content of TNF- 伪 -NF- 魏 B in the small intestine, resulting in the increase of intestinal permeability. Intestinal mucosal barrier function is impaired.
【作者单位】：湖南师范大学体育学院;扬州大学体育学院;
【基金】：扬州市科技局2012科技攻关—社会发展科技攻关项目(2012112) 2016年国家重点研发计划课题(2016yfd0400603-02)
【分类号】：R87

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