运动员有氧运动后血清MG53与骨骼肌损伤诊断指标血清CK、Mb相关性研究

发布时间：2018-03-04 20:48

本文选题：EIMD　切入点：MG53　出处：《首都体育学院》2017年硕士论文　论文类型：学位论文

【摘要】：研究目的:常用于监控运动员运动性骨骼肌损伤程度的血液生化指标有血清肌酸激酶(CK)、肌红蛋白(Mb)、血尿素(BUN)等。最近研究发现一种存在于骨骼肌和心肌中的特异性修复蛋白MG53(Mitsugumin 53),MG53可以与其它几种肌肉特异性膜修复蛋白在功能上协同作用完成肌细胞膜损伤后的快速修复。在此研究基础上,本文旨在探讨运动员在不同的有氧运动方式和运动时间下,骨骼肌损伤后血清中MG53与血清肌酸激酶、肌红蛋白、血尿素的相关性。为运动性骨骼肌损伤的科学诊断提供理论支持。研究方法:选取16名男性运动员(平均年龄23.13±2.92岁,训练年限5.13±2.92年,国家二级运动员)在空腹安静状态下采集静脉血。隔天在实验室进行功率车耐力骑行90min运动,起始功率为50W,保持60rpm的转速下每分钟递增20W直至通气无氧阈出现对应的负荷量即为功率车负荷。运动前,空腹安静状态下采集静脉血;运动后0h、1h、4h、24h分别采集一次静脉血。间隔2周进行功率车高强度间歇运动1min60-90%无氧阈强度、间歇4min20-40%无氧阈强度蹬车共10组。采血方案同上。两次运动测试结束后间隔一周再次采集静脉血测定血清中CK、Mb、BUN、MG53蛋白含量。所有测得数据结果采用SPSS 22.0统计软件进行分析。研究结果:不同时间点与运动方式:(1)功率车耐力运动中,不同时间点间的血清CK不具有显著性差异(P0.05)。运动后1h、4h和24h的Mb分别较安静值与运动后即刻显著升高(p0.01)。运动后即刻BUN较安静值有所升高(p0.05),运动后1h和4h的BUN较安静值显著升高(p0.01)。运动后24h的BUN较运动后1h和4h的BUN显著降低(p0.01)。各时间点MG53值无显著差异(P0.05)。(2)高强度间歇运动中,不同时间点间的血清CK不具有显著性差异(P0.05)。运动后1h和24h的Mb较运动后即刻显著升高(p0.01)。不同时间点之间BUN不具有显著性差异(P0.05)。各时间点MG53值无显著差异(P0.05)。同一时间点不同运动方式:(1)运动后4小时,功率车耐力运动Mb显著高于高强度间歇运动(p0.05)。(2)运动后即刻、1小时、4小时功率车耐力运动BUN显著高于高强度间歇运动(p0.05)。MG53与各指标之间的相关性:(1)功率车耐力运动中,运动后4小时Mb与CK呈正相关(r=0.755 p0.01),运动后24小时Mb与CK呈正相关(r=0.711 p0.01),MG53在运动不同时间点与CK、Mb、BUN均没有相关性。(2)高强度间歇运动中,各个时间点CK与Mb呈不同程度的相关性,MG53在运动不同时间点与CK、Mb、BUN均没有相关性。研究结论:(1)功率车耐力运动与高强度间歇运动后,Mb、BUN有显著性改变。(2)功率车耐力运动后即刻、运动后4小时血清Mb、BUN变化显著。(3)人源MG53在两种有氧运动方式运动后即刻相比运动前含量增加。(4)人源MG53与不同方式有氧运动前、运动后即刻、运动后1小时、4小时、24小时的血清CK、Mb、BUN之间没有相关性。(5)有氧运动后血清人源MG53与血清CK、Mb变化周期不同。
[Abstract]:Objective: the blood biochemical indexes commonly used to monitor the degree of sports skeletal muscle injury in athletes include serum creatine kinase (CK), myoglobin (Mb), blood urea bun (BUNA) and so on. Recently, a specific type of blood biochemical index was found in skeletal muscle and myocardium. The sex repair protein (MG53(Mitsugumin 53) MG53 can function in synergy with other muscle-specific membrane repair proteins (MG53) to complete the rapid repair of muscle cell membrane damage. The purpose of this study was to investigate the levels of MG53 and creatine kinase, myoglobin in the serum of athletes after skeletal muscle injury under different aerobic exercise patterns and exercise time. Correlation of blood urea. To provide theoretical support for scientific diagnosis of sports skeletal muscle injury. Methods: 16 male athletes (mean age 23.13 卤2.92 years, training time 5.13 卤2.92 years) were selected. State level II athletes) collect venous blood in an empty stomach and quiet state. The next day they do 90 minutes of endurance cycling in the laboratory. The initial power is 50W, and the speed of 60rpm is increased by 20W per minute until the corresponding load of ventilation anaerobic threshold appears. Before exercise, venous blood is collected under the condition of empty stomach and quiet. Venous blood was collected at 0 h, 1 h, 4 h and 24 h after exercise. The intensity of anaerobic threshold was 60-90% at intervals of 2 weeks. There were 10 groups of intermittent 4 mins 20-40% anaerobic threshold strength pedaling. The blood collection plan was the same. After two exercise tests, venous blood was collected again one week after the end of the two exercise tests to determine the protein content of CKMb BUNMG53 in serum. All the measured data were carried out by SPSS 22.0 statistical software. Analysis. Results: in endurance exercise of power vehicles at different time points and movements, There was no significant difference in serum CK between different time points (P 0.05). Mb at 1 h and 24 h after exercise was significantly higher than that at 1 h and 24 h after exercise, respectively. The BUN was significantly increased at 1 h and 4 h after exercise, and the BUN at 1 h and 4 h after exercise was quieter than that at 1 h and 4 h after exercise. The BUN of 24 hours after exercise was significantly lower than that of BUN at 1 and 4 hours after exercise. There was no significant difference in MG53 value at different time points (P0.05. 2) in high intensity intermittent exercise. There was no significant difference in serum CK between different time points (P 0.05). Mb at 1 h and 24 h after exercise was significantly higher than that at 1 h and 24 h after exercise. There was no significant difference in BUN between different time points (P 0.05). There was no significant difference in MG53 value at different time points (P 0.05). 4 hours after the exercise, Motor vehicle endurance exercise (Mb) was significantly higher than that of high intensity intermittent exercise (P 0.05U. 2) the BUN of power vehicle endurance exercise was significantly higher than that of high intensity intermittent exercise (P 0.05U. MG53). There was a positive correlation between Mb and CK at 4 hours after exercise, and a positive correlation between Mb and CK at 24 hours after exercise. There was no correlation between Mb and CK at different time points. There was no correlation between CK and Mb at different time points and MG53 had no correlation with CKMb bun at different time points. Conclusion: the endurance exercise of power vehicle and the high intensity intermittent exercise have significant changes. After 4 hours of exercise, the change of serum Mbbun was significant. 3) the content of human MG53 increased immediately after two aerobic exercise modes compared with that before exercise. The content of human MG53 was higher than that before and immediately after aerobic exercise in different ways, and immediately after exercise, the content of human MG53 was increased immediately after exercise. There was no correlation between serum CKB and Mbbun at 1 hour, 4 hours and 24 hours after exercise. (5) the period of change of serum MG53 was different from that of serum CK Mb after aerobic exercise.
【学位授予单位】：首都体育学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R873

【相似文献】