一次长时间运动对MAPK信号的影响

发布时间：2018-03-09 04:23

本文选题：一次长时间运动　切入点：骨骼肌　出处：《北京体育大学》2012年硕士论文　论文类型：学位论文

【摘要】：研究目的：本研究通过建立一个耐力运动模型,探讨一次长时间运动后MAPK信号的变化规律,及其在骨骼肌蛋白合成中的作用。研究方法：9周龄雄性SD大鼠42只,采用自由饮食喂养。适应性训练后,分为7组,每组6只：安静对照组(C),90分钟运动后即刻取材组(GO),90分钟运动后1时取材组(G1),90分钟运动后6时取材组(G2),]20分钟运动后即刻取材组(LO)120分钟运动后1时取材组(L1),120分钟运动后6时取材组(L2)。实验为一次长时间训练：除安静组外所有大鼠均需在水平跑台一次长时间训练,坡度5°,跑速20m/min,运动90min和120min,共计运动一次.所有组在运动后即刻,1时,6时取样。用Western blotting方法测定骨骼肌中MEK1/2, ERK1/2, P90rsk磷酸化表达,MHC蛋白表达。研究结果： (])与安静对照组相比,G2组和L2组MEK1/2磷酸化水平(P0.05),显著性下降。G1组磷酸化增加。G组运动后1时磷酸化水平增加,6时下降。一次长时间运动使骨骼肌MEK1/2的磷酸化水平改变,使G组先降低后升高再降低,L组逐渐降低。 (2)与安静组C相比,G2组ERK1/2磷酸化(P0.05),显著性下降。90分钟运动组和120分钟运动组磷酸化水平均稍低于安静对照组,两运动组趋势一致,均为运动后1时比运动后即刻磷酸化水平升高,运动后6时降低。 (3)与安静组C相比,L(L0.L1.L2)组P90rsk磷酸化(P0.05),显著性降低。90分钟运动组和120分钟运动组磷酸化水平均低于安静对照组。 (4)与安静组C相比,G2组MHC磷酸化(PO.05),显著性降低。90分钟运动组的趋势为运动后1时比运动后即刻升高,运动后6时明显下降。120分钟运动组运动后即刻与运动后1时比安静对照组降低,但6时明显升高研究结论：一次性长时间运动使MAPK信号通路中MEK1/2磷酸化活性降低,ERK1/2磷酸化活性降低,p90rsk磷酸化活性降低,骨骼肌MHC蛋白合成降低。
[Abstract]:Objective: to establish an endurance exercise model to study the changes of MAPK signal after a long time exercise and its role in skeletal muscle protein synthesis. Methods Forty-two male Sprague-Dawley rats aged 9 weeks were fed with free diet. After adaptive training, they were divided into 7 groups. Six rats in each group: the quiet control group immediately after 90 minutes of exercise, the control group immediately after 90 minutes of exercise, the 1:00 sampling group after 90 minutes of exercise, the 1:00 selection group, the material selection group after 90 minutes of exercise, the 6:00 sampling group, the group of G _ 2s, the immediately after 20 minutes of exercise, the immediate sampling group, after 120 minutes of exercise, the 1:00 sampling group, the L1120 minutes exercise, and the latter, the taking group L1120 minutes of exercise. The experiment was conducted for a long time: all the rats except the quiet group were trained for a long time on the horizontal running platform. All groups were sampled at 1:00 and 6:00 immediately after exercise. MEK1 / 2, ERK1 / 2, P90rsk phosphorylated expression and MHC protein expression in skeletal muscle were determined by Western blotting method. Results of the study:. Compared with the quiet control group, the phosphorylation level of MEK1/2 in G 2 group and L 2 group was significantly decreased. The phosphorylation level in G 1 group increased after exercise. The phosphorylation level in G group increased at 1:00 and decreased at 6:00. One long time exercise changed the level of MEK1/2 phosphorylation in skeletal muscle. Group G decreased first and then increased and then decreased gradually. (2) compared with the rest group C, the phosphorylation level of ERK1/2 phosphorylation in the G 2 group was significantly lower than that in the rest control group, and the phosphorylation levels in the exercise group and the 120 minute exercise group were slightly lower than those in the rest control group, and the trend of the two exercise groups was the same. The level of phosphorylation increased at 1:00 after exercise and decreased at 6:00 after exercise. The phosphorylation level of P90rsk phosphorylated P90rsk in the control group was significantly lower than that in the control group (P < 0.05). The phosphorylation levels in the exercise group and the 120min exercise group were significantly lower than those in the control group. (4) compared with the rest group C, the trend of MHC phosphorylation in G _ 2 group was significantly lower than that in the control group (P < 0.05). The trend of exercise group was that 1:00 after exercise was higher than that immediately after exercise. Significantly decreased at 6:00 after exercise .120min exercise group immediately after exercise and 1:00 after exercise compared with the quiet control group, but 6:00 significantly increased. The study concluded that:. Long time exercise decreased the phosphorylation activity of MEK1/2 in MAPK signaling pathway. The phosphorylation activity of ERK1 / 2 decreased the phosphorylation activity of p90rsk and the synthesis of MHC protein in skeletal muscle.
【学位授予单位】：北京体育大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：G804.2

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