大鼠运动性骨骼肌损伤后血清肌酸激酶、乳酸脱氢酶以及波形蛋白的变化

发布时间：2018-03-09 12:47

本文选题：离心运动　切入点：骨骼肌损伤　出处：《中国老年学杂志》2016年15期 　论文类型：期刊论文

【摘要】：目的探讨大鼠离心运动致骨骼肌损伤后血清肌酸激酶(CK)、乳酸脱氢酶(LDH)以及波形蛋白(Vimentin)的变化。方法 SPF级健康雄性SD大鼠40只,随机分为对照组和实验组各20只。实验组根据运动后时间不同分为运动后即刻(实验组A)、运动后12 h(实验组B)、运动后24 h(实验组C)、运动后48 h(实验组D),每组5只。观察大鼠离心运动情况,各组大鼠血清CK和LDH水平变化,各组大鼠骨骼肌Vimentin蛋白表达情况。结果与对照组相比,实验组A、实验组B、实验组C大鼠血清CK、LDH水平明显上升,且呈依次增加趋势(P0.05),而实验组D无明显变化(P0.05);与实验组A、实验组B、实验组C比较,实验组D大鼠血清CK、LDH水平明显下降(P0.05);与对照组比较,实验组A、实验组B、实验组C大鼠骨骼肌Vimentin表达阳性强度吸光度值明显增加(P0.05),且呈依次下降趋势,而实验组D无明显变化(P0.05);与实验组A、实验组B、实验组C比较,实验组D大鼠骨骼肌Vimentin表达阳性强度吸光度值明显降低(P0.05)。结论大鼠离心运动能够引起延迟性骨骼肌损伤,CK、LDH、Vimentin可更好反映骨骼肌损伤的程度。
[Abstract]:Objective to investigate the changes of serum creatine kinase (CK), lactate dehydrogenase (LDH) and vimentin (Vimentin) in skeletal muscle after eccentric exercise in rats. The experimental group was randomly divided into two groups: the control group (n = 20) and the experimental group (n = 20). The experimental group was divided into two groups according to the time after exercise: immediately after exercise (experimental group A, 12 h after exercise) (experimental group B, 24 h after exercise, experiment group C, 48 h after exercise) (experimental group D, n = 5, each group, n = 5). To observe the centrifugal exercise in rats, The changes of serum CK and LDH levels and the expression of Vimentin protein in skeletal muscle of rats in each group were observed. Results compared with the control group, the serum levels of CK and LDH in experimental group A, experimental group B and experimental group C were significantly increased. Compared with experimental group A, experimental group B and experimental group C, the level of serum CK LDH in experimental group D decreased significantly (P 0.05), and compared with control group, compared with control group, the level of LDH in serum of experimental group D decreased significantly. The positive intensity absorbance of Vimentin expression in the skeletal muscle of experimental group A, experimental group B, experimental group C was significantly increased and decreased in turn, but the change of group D was not obvious (P 0.05), which was compared with experimental group A, experimental group B and experimental group C, and compared with experimental group A, experimental group B, experimental group C, and compared with experimental group A, experimental group B, experimental group C. The positive intensity absorbance of Vimentin expression in the skeletal muscle of experimental group D decreased significantly (P 0.05). Conclusion eccentric exercise can induce delayed skeletal muscle injury and can better reflect the degree of skeletal muscle injury.
【作者单位】：浙江工业职业技术学院;浙江大学附属第一医院病理科;浙江大学附属第一医院骨科;松原市中心医院病理科;温州医学院运动医学学院;
【基金】：浙江省自然科学基金课题(No.LY12C11002) 温州医学院科研启动基金课题(No.QTJ09018)
【分类号】：R873

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