高灵敏、高对比度无标记三维光学微血管造影系统与脑科学应用研究

发布时间：2018-03-09 18:57

本文选题：光学相干层析术微血管造影　切入点：小波域分量复合　出处：《物理学报》2016年15期 　论文类型：期刊论文

【摘要】：结合光学相干层析技术的三维成像能力和动态散射技术的运动识别能力,可以实现无标记的三维光学微血管造影,在不牺牲线扫描速度的前提下,通过帧间分析的方法提高血流造影的灵敏度,实现毛细血管水平的探测.提出小波域分量复合的方法降低静态组织信号和动态血流信号之间的分割误差,实现高对比度的血管造影分别利用组织血流模拟样品和活体大鼠脑组织进行实验验证,结果发现,采用小波域分量复合之后,血管分割误差分别减小了83%和71%,造影图对比度增强,并且具有更好的血管连接性.进而,利用研制的系统对大鼠脑血管局部缺血性中风模型进行了初步的成像研究,清晰地呈现了中风模型形成,血管受损和血管恢复的整个过程,有助于对局部缺血性中风模型机理的研究.
[Abstract]:Combined with the 3D imaging ability of optical coherence tomography and the motion recognition ability of dynamic scattering technique, unlabeled 3D optical microangiography can be realized without sacrificing the scanning speed of line. The sensitivity of blood flow angiography is improved by inter-frame analysis, and the detection of capillary level is realized. A method of wavelet domain component recombination is proposed to reduce the segmentation error between static tissue signal and dynamic blood flow signal. The high contrast angiography was verified by tissue blood flow simulation sample and rat brain tissue respectively. The results showed that, after wavelet domain component recombination, The errors of vascular segmentation were reduced by 83% and 71, respectively. The contrast of the angiogram was enhanced, and the vascular connectivity was improved. Furthermore, a preliminary imaging study was carried out on the cerebral ischemic stroke model in rats using the developed system. It clearly presents the whole process of stroke model formation, vascular damage and vascular recovery, which is helpful to the study of the mechanism of local ischemic stroke model.
【作者单位】：浙江大学现代光学仪器国家重点实验室光电科学与工程学院;
【基金】：浙江省自然科学基金(批准号:LY14F050007) 国家自然科学基金(批准号:61475143,11404285,61335003,61327007,61275196) 浙江省科技厅公益性技术应用研究计划(批准号:2015C33108) 国家高技术研究发展计划(批准号:2015AA020515) 中央高校基本科研业务费专项资金(批准号:2014QNA5017) 教育部留学回国人员科研启动基金资助的课题~~
【分类号】：R816.1

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