递增循环载荷量致骨腱结合部过度使用损伤的发病机制研究

发布时间：2018-03-11 13:51

  本文选题：递增　切入点：循环　出处：《北京体育大学》2014年博士论文　论文类型：学位论文

【摘要】：目的：通过递增循环载荷量致过度使用损伤发生,在此过程从形态结构、生长因子、急性期炎性蛋白及细胞外基质表达的变化,探索髌骨-膑腱结合部(Patella-Patella Tendon Junction, PPTJ)过度使用损伤的发病机制。 方法：45只成年雌性新西兰大白兔,按循环载荷量不同随机分成2h、4h、6h、12h和24h组。完成相应的循环载荷量后,处死、取材、常规组织学处理。行HE、Safrain0染色以及CRP、生长因子和细胞外基质的免疫组化染色。MetaMorph Premier Offline对各组肌腱厚度、细胞密度、纤维软骨带厚度、胶原排列、粘多糖蛋白面积指标组织形态计量。免疫组化的评定包括标本阳性染色率及阳性染色细胞密度。SAS6.1.2进行统计分析处理,显著性水平为P0.05。 结果：与C相比：6h组细胞密度显著增加(p0.01),12h、24h组纤维软骨带显著增宽(p0.01),4h、6h、12h和24h组胶原排列显著紊乱(p0.01)；24h组肌腱厚度显著低于12h组(p0.05)；4h组b-FGF阳性染色细胞密度显著高于其它各组(p0.05)；C组CTGF阳性染色细胞密度显著低于其它各组(p0.05)；2h组TGF-β2、PDGF-B阳性染色细胞密度均显著高于C、6h、12h和24h组(p0.05)；随循环载荷量增加：VEGF的阳性染色率显著增加(p0.05); BMP-7标本阳性染色呈现线性增加趋势(p0.05); Tenascin-C的阳性染色细胞密度呈显著的二次多项式趋势(p0.05)。与C组相比,2h和24h组Collagen-III阳性染色细胞密度显著升高(p0.05)。 结论：149759±7285N/kg体重的循环载荷量能成功建立PPTJ过度使用损伤动物模型。在过度使用损伤发生过程中,PPTJ先后呈现出胶原排列紊乱-细胞密度增加-纤维软骨带增宽的病理变化过程。VEGF表达的增加使PPTJ血管增生,bFGF的降低使得PPTJ修复能力下降,TGF-β2表达减少降低肌腱的抗牵拉能力,CTGF的持续高表达引起PPTJ胶原的交联,四者表达的变化可能促使过度使用损伤发生。Ⅲ型胶原表达增加加剧了PPTJ过度使用损伤发展,Tenascin-C表达的降低使得细胞外基质的机械稳定性降低,二者表达变化可能促进过度使用损伤的发生。
[Abstract]:Aim: to change the expression of inflammatory proteins and extracellular matrix (ECM) in the process of overuse injury caused by increasing cyclic load, including morphological structure, growth factor, inflammatory protein and extracellular matrix. Objective: to explore the pathogenesis of patella-patella Tendon Junction (PPTJ) overuse injury in patellar patellar tendon junction. Methods Forty-five adult female New Zealand white rabbits were randomly divided into two groups according to their cyclic load. Routine histopathology. Hefa Safrain0 staining and immunohistochemical staining of CRP, growth factor and extracellular matrix .Meta Morph Premier Offline on tendon thickness, cell density, fibrochondral band thickness, collagen arrangement in each group. Tissue morphometry of mucopolysaccharide protein area index. Immunohistochemical evaluation included positive staining rate and positive staining cell density. SAS6.1.2 for statistical analysis, the significant level was P0.05. Results: compared with C group, the cell density of group C was significantly higher than that of group C for 12 h and 24 h. The proliferation of fibrochondrocytes was significantly wider in group P 0.01C for 4 h and in group C for 24 h. The thickness of tendon in group 24 h was significantly lower than that in group C (group C) was significantly lower than that in group C (24 h), and the density of b-FGF positive cells in group 24 h was significantly higher than that in group B (0.05 h). The cell density of CTGF positive staining in group C was significantly lower than that in group C (2 h), and the density of positive cells in group C was significantly higher than that in group C (12 h and 24 h), the positive staining rate of CTGF was significantly increased with the increase of cyclic load, and the positive staining rate of BMP-7 was significantly higher than that of group C (P 0.05). The positive cell density of TGF- 尾 2 + PDGF-B in group C was significantly higher than that in group C at 6 h and 24 h, and the positive staining rate of CTGF was significantly increased with the increase of cyclic load. The positive staining cell density of Tenascin-C showed a significant quadratic polynomial trend. Compared with group C, the density of Collagen-III positive staining cells in group C was significantly higher than that in group C (2 h and 24 h). Conclusion the PPTJ model of overuse injury can be successfully established by the cyclic load of 1: 149759 卤7285N-1 / kg body weight. In the process of overuse injury, the disorder of collagenous arrangement, cell density increase and fibrochondral band widening can be seen successively in the process of overuse injury. The increased expression of VEGF decreased the repair ability of PPTJ and decreased the expression of TGF- 尾 2. The continued high expression of TGF- 尾 2 resulted in the cross-linking of PPTJ collagen. Increased expression of type 鈪，

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