放疗对小型猪腮腺颌下腺舌下腺微血管损伤影响的实验研究

发布时间：2018-03-26 05:32

本文选题：腮腺　切入点：颌下腺　出处：《口腔医学研究》2017年01期

【摘要】：目的:观察放疗对小型猪腮腺、颌下腺、舌下腺的微血管损伤状况。方法:将6只实验用小型猪分为2组。2组动物进行放疗,将双侧腮腺、颌下腺、舌下腺加入放射野中,放疗组20Gy/每侧,对照组0Gy/每侧。放疗结束后4h处死两组动物,取两组动物腮腺、颌下腺、舌下腺标本行HE染色与CD31免疫组化染色,观察放疗早期3种腺体微血管密度变化。结果:两组小型猪腮腺、颌下腺、舌下腺CD31阳性染色颗粒数有显著差异(P0.05);3个腺体间统计结果有显著性差异(P0.05);腮腺与颌下腺及舌下腺有显著性差异(P0.05),颌下腺与舌下腺无显著性差异。结论:放疗可导致小型猪腮腺、颌下腺、舌下腺微血管密度明显减低,且各腺体间微血管密度减低有差异,腮腺的损伤程度大于颌下腺和舌下腺。
[Abstract]:Objective: to observe the microvascular injury of parotid, submandibular and sublingual glands in miniature pigs treated by radiotherapy. Methods: six small pigs were divided into 2 groups, and the bilateral parotid gland, submandibular gland and sublingual gland were added to the radiation field. Each side of 20Gy/ in radiotherapy group and 0Gy/ side in control group were killed 4 hours after radiotherapy. The parotid gland, submandibular gland and sublingual gland of the two groups were collected for HE staining and CD31 immunohistochemical staining. The changes of microvessel density of three kinds of glands in the early stage of radiotherapy were observed. Results: the parotid gland and submandibular gland of the two groups were observed. There were significant differences in the number of CD31 positive granules in sublingual gland (P 0.05), statistical difference among three glands (P 0.05), significant difference between parotid gland and submandibular gland and sublingual gland (P 0.05), but no significant difference between submandibular gland and sublingual gland. Conclusion: radiotherapy can lead to parotid gland in miniature pig. The microvessel density of submandibular gland and sublingual gland was significantly decreased, and the microvessel density of each gland was different. The degree of injury of parotid gland was greater than that of submandibular gland and sublingual gland.
【作者单位】：首都医科大学附属北京友谊医院口腔科;北京市隆福医院口腔科;
【基金】：国家自然科学基金(编号:81070844) 北京市卫生系统高层次卫生技术人才培养计划项目(编号:2013-3-065)
【分类号】：R739.91;R730.55

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