6周递增跑台运动对大鼠肾上腺ACTH、GC受体和血清GC的影响

发布时间：2018-03-28 20:50

本文选题：递增负荷运动　切入点：促肾上腺皮质激素受体mRNA　出处：《西安体育学院学报》2017年01期

【摘要】：目的探讨6周递增跑台运动对大鼠血清GC和肾上腺ACTH、GC受体的影响。方法 SPF级雄性SD大鼠128只,随机分成16组,分别是完全不训练组(A),共4小组,每小组8只;处死当天不训练组(B),共4小组,每小组8只;运动后即刻处死组(C),共4小组,每小组8只;运动后3 h处死组(D),共4组,每小组8只。A组在整个实验过程中不训练,B、C、D组进行6周递增负荷跑台运动。分别在第0、2、4、6周周末处死A、B、C、D组各一小组。取肾上腺和血液,用Real-time PCR测试各小组肾上腺ACTH-R mRNA和GR mRNA的表达,用ELISA法测试各小组血清中GC的含量。结果第0、2、4、6周末4组大鼠的肾上腺中ACTH-R mRNA在第0周、第2周末和第6周末的表达没有显著性差异(P㧐0.05),只是在第4周末B组和C组比A组和D组有显著性升高(P0.05);各组GR mRNA在第0周和第2周末无显著性差异,第4周末和第6周末的C组和D组比其它两组有极显著性升高(P0.001);各周末血清中GC含量都表现为C组和D组比其它两组有显著性升高(P0.05)。结论 6周递增负荷运动过程中,肾上腺ATCH-R mRNA表达表现出时相性和饱和性,其表达并没有和ACTH阳性细胞的表达保持一致,二者具有不同步性,可能是ACTH在运输或在正、负反馈调节环节受到调控的结果。随着运动强度的增大,机体的中GR mRNA表达的含量降低,其表达趋势和GC分泌趋势相反,这应该是机体保持自身稳态的保护机制之一。
[Abstract]:Objective to investigate the effect of 6-week incremental treadmill exercise on serum GC and adrenal ACTHN GC receptors in rats. Methods SPF grade male SD rats were randomly divided into 16 groups with 8 rats in each group. On the day of death, 4 groups (8 rats per group) were killed without training, 4 groups (8 rats) were killed immediately after exercise, 4 groups (8 rats per group) were killed immediately after exercise, and 4 groups (4 groups) were killed 3 hours after exercise. During the whole experiment, 8 rats in group A were killed at the end of the 6th week, and the adrenal gland and blood were taken from adrenal gland and blood. The expression of ACTH-R mRNA and gr mRNA in adrenal gland was measured by Real-time PCR, and the content of GC in serum was measured by ELISA method. Results ACTH-R mRNA in adrenal gland of 4 groups was detected at the end of the 6th week. There was no significant difference in expression between the 2nd weekend and the 6th weekend. There was no significant difference in gr mRNA between group B and group C at the end of the 4th week compared with that in group A and group D, but there was no significant difference in gr mRNA between group A and group D at the end of week 0 and week 2, but there was no significant difference between group B and group C at the end of the 4th week. At the end of the 4th and 6th weekend, the serum GC levels in group C and group D were significantly higher than those in group C and group D compared with the other two groups. Conclusion during 6 weeks of incremental load exercise, the serum GC levels in group C and group D were significantly higher than those in group C and group D, respectively. Conclusion during 6 weeks of incremental load exercise, the serum GC levels in group C and D were significantly higher than those in group C and group D, respectively. The expression of ATCH-R mRNA in adrenal gland showed chronotropic and saturation, and its expression was not consistent with the expression of ACTH positive cells. The expression of ACTH was different from that of ACTH positive cells, and it may be that the expression of ACTH was in transit or positive. With the increase of the intensity of exercise, the expression of gr mRNA in the body decreased, which was contrary to the tendency of GC secretion, which should be one of the protective mechanisms to maintain the homeostasis of the body.
【作者单位】：山东师范大学体育学院;华南师范大学体育科学学院;
【基金】：广东省自然科学基金项目(9151063101000059)
【分类号】：R87

【参考文献】