钝挫伤对大鼠骨骼肌卫星细胞体外增殖的影响

发布时间：2018-03-29 11:34

本文选题：骨骼肌　切入点：钝挫伤　出处：《中国老年学杂志》2017年05期

【摘要】：目的研究钝挫伤对大鼠骨骼肌卫星细胞体外增殖的影响。方法选取24只5周龄Wistar雄性大鼠,随机分为安静对照组(C组)和钝挫伤即刻(D0组)、24 h(D24组)、48 h组(D48组)4组,每组6只。其中钝挫伤组进行钝挫伤的操作后,分别于即刻、24、48 h进行麻醉,与对照组一同摘取左侧腓肠肌,立刻进行肌卫星细胞体外培养。另取新生鼠(N组)2只,宰杀后取下左侧腓肠肌,同样进行肌卫星细胞体外培养。结果新生鼠骨骼肌卫星细胞增殖最显著,在培养第2天便可见少量纺锤形的单核细胞出现;在第3天就大量增殖,当培养5 d后,卫星细胞相互融合形成多核的肌管;D0组3 d后可见肌卫星细胞,而D24、D48组4 d后可见卫星细胞,到第8天时增殖达到高峰,基本布满大部分培养皿,部分分化融合成肌管,之后增殖速度下降。C组肌卫星细胞含量最少,且增殖速度最慢。结论钝挫伤对骨骼肌卫星细胞的激活与增殖具有重要作用,且新生鼠体内的肌卫星细胞并未处于静息状态。
[Abstract]:Objective to study the effect of blunt contusion on the proliferation of skeletal muscle satellite cells in vitro. Methods Twenty-four Wistar male rats aged 5 weeks were randomly divided into two groups: control group C (n = 24) and group D _ 0 (n = 48 h) and control group (n = 4). There were 6 rats in each group. After the operation of blunt contusion, the left gastrocnemius muscle was removed from the left gastrocnemius muscle with the control group for 48 hours, and the satellite cells were cultured in vitro. The left gastrocnemius muscle was removed from the left gastrocnemius and cultured in vitro. Results the proliferation of skeletal muscle satellite cells in newborn rats was the most significant, and a small number of spindle monocytes appeared on the second day of culture, and a large number of proliferation occurred on the third day. After 5 days of culture, the satellite cells were fused to form multicellular myotube D0 group after 3 days, while the satellite cells were found in D24 D48 group after 4 days. At the 8th day, the proliferation of satellite cells reached its peak and was basically covered with most of the petri dishes. After partial differentiation and fusion into myotube, the proliferation rate of muscle satellite cells in group C was the least, and the proliferation rate was the slowest. Conclusion blunt contusion plays an important role in the activation and proliferation of skeletal muscle satellite cells. Moreover, the muscle satellite cells in newborn mice were not in a resting state.
【作者单位】：湖南医药学院体育教研室;扬州大学;
【基金】：湖南医药学院校级科研课题(20152KB15)
【分类号】：R873

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