海带多糖对大鼠颌下腺血管辐射损伤防护作用的初步研究

发布时间：2018-03-31 11:11

本文选题：颌下腺　切入点：海带多糖　出处：《广西医科大学》2013年硕士论文

【摘要】：目的放疗为头颈部肿瘤常用治疗方式之一。由于涎腺解剖位置及其周组织紧密毗邻,在治疗过程中辐射损伤无法避免。目前射线引发腺泡和导管细胞的凋亡为研究热点,但腺体其他附属组织如血管、神经、淋巴等的损伤亦不容忽视。血管为腺体组织汲取营养及新陈代谢的重要媒介,射线引起腺体血管的损伤及闭锁可加剧病情恶化。褐藻类萃取物海带多糖(laminaria japonica polysaccharide, LJP)天然且毒副作用小,具有抗辐射、调节免疫、抗氧化等许多生物活性。本研究将探讨海带多糖对60Coγ射线所诱导的大鼠颌下腺血管损伤的防护作用。方法70只Wistar大鼠随机分为4组：正常对照组(N组),放疗对照组(R组),海带多糖高剂量(300mg/kg)照射组(LHR组),海带多糖低剂量(30mg/kg)照射组(LLR组)。照射前3天及照射后3天分别给予腹腔注射生理盐水和海带多糖。R组,LHR组,LLR组的大鼠头颈部予以60Coγ射线15Gy一次性照射。免疫组织化学方法检测腺体微血管密度(microvessel density, MVD); RT-PCR检测血管内皮生长因子(vascular endothelial growth factor, VEGF)、基质金属蛋白酶9(matrix metalloproteinase-9,, MMP-9)及基质金属蛋白酶抑制剂1(tissue inhibitor ofmetalloproteinase-1, TIMP-1)的表达,其扩增产物经电泳后行凝胶成像分析；透射电镜观察颌下腺血管内皮细胞超微结构改变。结果 ①免疫组织化学检测见海带多糖可降低照射后的颌下腺微血管密度的减小幅度,LHR和LLR组的MVD与R组相比差异有统计学意义(P0.05)； ②RT-PCR检测VEGF、MMP-9在LHR及LLR组的表达均高于R组(P0.05), TIMP-1在各组表达差异无统计学意义(P0.05)； ③电镜下观察R组血管及内皮细胞损伤最为明显,LHR及LLR组损伤较轻。结论海带多糖可减轻射线对颌下腺血管的损伤。
[Abstract]:The purpose of radiotherapy of head and neck cancer is one of the commonly used treatment. Because salivary gland anatomical site and periodontal tissue closely adjacent to the injury can not be avoided in the course of treatment. The radiation ray induce apoptosis of acinar and duct cells as the research hotspot, but the glands and other affiliated organizations such as blood vessels, nerves, lymph and other damage should not be ignored. Vascular glands learn the important nutrition and media organization caused by radiation damage and The new supersedes the old., atresia gland blood vessel can aggravate disease progression. The brown algae extract of Laminaria japonica polysaccharides (Laminaria japonica polysaccharide, LJP) natural and low adverse effect, with anti radiation, regulating immunity, antioxidant and many other biological activities. This study will explore the protective effect of Laminaria japonica polysaccharide induced by 60Co gamma ray submandibular vascular injury in rats.
Methods 70 Wistar rats were randomly divided into 4 groups: normal control group (N group) and radiotherapy group (R group), kelp polysaccharide high dose (300mg/kg) irradiation group (LHR group), low dose kelp polysaccharide (30mg/kg) irradiation group (LLR group). 3 days before irradiation and irradiation after 3 days were given intraperitoneal injection of saline and laminarin.R group, LHR group, LLR group of rat neck to 60Co gamma ray irradiation. 15Gy immunohistochemical method to detect microvessel density (microvessel gland density, MVD RT-PCR); vascular endothelial growth factor (vascular endothelial, growth factor, VEGF), matrix metalloproteinase 9 (matrix, metalloproteinase-9, MMP-9) and tissue inhibitor of matrix metalloproteinase 1 (tissue inhibitor, ofmetalloproteinase-1, TIMP-1) expression of the amplified products were electrophoresis gel imaging analysis; observe vascular endothelial cell ultrastructure by transmission electron microscopy of submandibular gland Structural changes.
Result
(1) immunohistochemistry showed that Laminaria Polysaccharide decreased the microvessel density of submandibular gland after irradiation. There was a significant difference in MVD between LHR and LLR group compared with R group (P0.05).
(2) the expression of VEGF by RT-PCR and the expression of MMP-9 in LHR and LLR groups were higher than that in group R (P0.05), and there was no significant difference in the expression of TIMP-1 in each group (P0.05).
(3) the most obvious damage of vascular and endothelial cells in R group was observed under electron microscope, and the injury of group LHR and LLR was lighter.
Conclusion the polysaccharide of kelp can reduce the damage to the blood vessels of the submandibular gland.

【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R965;R818.05

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