运动性低血色素形成中大鼠十二指肠铁吸收蛋白表达的动态变化

发布时间：2018-04-02 18:34

本文选题：运动性低血色素　切入点：血红素转运蛋白1　出处：《河北师范大学》2012年硕士论文

【摘要】：铁在运动中发挥重要作用。运动性低血色素的发生往往严重影响运动员的运动能力。机体主要通过调控十二指肠铁吸收来维持机体铁稳态。血红素转运蛋白1(heme carrier protein1，HCP1)、二价金属离子转运体(divalent metal transporter1，DMT1)及膜铁转运蛋白1（Ferroportin1，FPN1）是近年来发现的肠铁吸收相关蛋白，参与十二指肠铁的吸收和释放并在肠铁吸收转运中起关键作用。HCP1和DMT1可分别将食物中的血红素铁和非血红素铁转运到十二指肠上皮吸收细胞内，，而FPN1将十二指肠上皮细胞内的铁转运到胞外，通过血液循环将铁运输至各个器官，供机体进行各种生理活动。然而运动中肠铁吸收的具体变化过程还不甚清楚，尤其是运动性低血色素形成中机体通过何途径如何调节小肠铁转运蛋白的表达，还需进一步研究。因此，本文研究了长时间递增负荷跑台运动导致的运动性低血色素形成中，大鼠肠铁吸收蛋白HCP1、DMT1和FPN1表达的动态变化，为运动性低血色素的预防和治疗提供实验和理论依据。研究内容与方法 36只雄性Wistar大鼠，随机分为对照组（control group，CG）和运动组（exercisegroup，EG）。对照组和运动组又分别分为3周、4周、5周组，运动组大鼠进行6d/w、坡度为0、速度30m/min的递增负荷跑台训练。前2周每天训练1次，时间从1min开始，每次递增2min，从第3周开始，每天训练2次。分别于运动第3、4、5周末取材，与其相对应的对照组也同时取材。采用血细胞自动分析仪测定血红蛋白（Hb）含量；分光光度计测血清铁含量；Western Blot检测十二指肠上皮细胞HCP1、DMT1及FPN1表达。研究结果 1、运动性低血色素形成中大鼠血红蛋白（Hb）的变化：经过长时间大强度运动后大鼠Hb含量逐渐降低。运动组大鼠3周Hb与其对照组相比有下降趋势，4周和5周显著低于其对照组（P 0.05，P 0.01）。 2、运动性低血色素形成中大鼠血清铁含量的变化：经过递增负荷运动后大鼠血清铁含量先增加后降低。运动组大鼠3周血清铁含量与其对照组相比显著增加（P 0.05）；4周运动组大鼠血清铁含量与其对照组相比稍有减少但变化不明显；5周运动组大鼠血清铁含量与其对照组相比极显著减少（P 0.01）。 3、运动性低血色素形成中大鼠十二指肠上皮吸收细胞铁吸收蛋白的变化：运动组大鼠3周十二指肠上皮细胞HCP1、DMT1及FPN1表达均显著高于其对照组（P0.01），4周与其对照组相比均无显著差异（P0.05），5周均显著低于对照组（P0.01）。研究结论长时间大强度运动开始阶段，机体通过增加十二指肠铁吸收维持运动机体对铁的需求，但随运动时间延长，机体肠铁吸收和释放能力降低，导致血清铁含量减少，这是引发运动性低血色素的重要原因之一。
[Abstract]:Iron plays an important role in sports.The occurrence of sports hypohemoglobin often seriously affects the athletic ability of athletes.The body maintains iron homeostasis by regulating the absorption of iron in the duodenum.The heme transporter 1(heme carrier protein 1 (HCP1), divalent metal transporter1 (DMT1) and membrane iron transporter (1) Ferroportin 1 (FPN1) are identified as iron absorption related proteins in the intestine.Participate in the absorption and release of iron from duodenum and play a key role in the absorption and transport of iron in intestine. HCP1 and DMT1 can transport heme iron and non heme iron from food into the absorption cells of duodenal epithelium, respectively.FPN1 transports iron from the duodenal epithelial cells to the extracellular, and transports iron to various organs through blood circulation for various physiological activities.However, the specific process of iron absorption in the intestine during exercise is not clear, especially the regulation of the expression of small intestinal iron transporter by the body in the process of exercise hypohemoglobin formation, which needs further study.Therefore, the dynamic changes of the expression of iron absorption protein HCP1DMT1 and FPN1 during the formation of exercise hypohemoglobin induced by prolonged incremental treadmill exercise in rats were studied in order to provide experimental and theoretical basis for the prevention and treatment of exercise hypohemoglobin.Research contents and methodsThirty-six male Wistar rats were randomly divided into two groups: control group (CGG) and exercise group (exercise group).The control group and the exercise group were divided into 3 weeks and 4 weeks and 5 weeks groups respectively. The rats in the exercise group were trained with incremental load running for 6 d / w with a slope of 0 and a speed of 30m/min.The first 2 weeks were trained once a day, the time began from 1min, each time increased by 2 mins, from the 3rd week, 2 times a day.The materials were collected at the end of the 5th week of exercise, and the control group was also taken at the same time.The content of hemoglobin (HB) and the expression of HCP1DMT1 and FPN1 in duodenal epithelial cells were determined by automatic blood cell analyzer and serum iron content by spectrophotometer and Western Blot respectively.Research results1. The changes of hemoglobin (HB) in rats during exercise induced hypohemoglobin formation: after a long time and intensive exercise, the HB content of rats decreased gradually.Compared with the control group, HB in the exercise group decreased significantly at 4 and 5 weeks, which was significantly lower than that in the control group at 4 and 5 weeks.2. Changes of serum iron content in exercise induced hypohemoglobin formation: after increasing load exercise, the serum iron content of rats increased first and then decreased.The serum iron content in the exercise group was significantly higher than that in the control group at 3 weeks. The serum iron content in the exercise group was slightly lower than that in the control group, but there was no significant change in the serum iron content between the exercise group and the control group.Compared with the control group, there was a significant decrease of P 0.01.3, changes of iron absorption protein in duodenal epithelial resorption cells of rats during exercise hypohemoglobin formation: the expression of HCP1DMT1 and FPN1 in duodenal epithelial cells in exercise group was significantly higher than that in control group at 4 weeks after 3 weeks of exercise compared with that in control group.There was no significant difference in P0. 05 and P0. 01 in the control group at 5 weeks.Research conclusionAt the beginning of a long period of high intensity exercise, the body maintained the iron demand by increasing the absorption of iron in duodenum, but with the extension of exercise time, the absorption and release ability of iron in intestine decreased, which resulted in the decrease of iron content in serum.This is one of the important causes of exercise-induced hypohemoglobin.
【学位授予单位】：河北师范大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R87

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