益美肤治疗SD大鼠急性β射线皮肤Ⅱ度损伤创面的实验研究

发布时间：2018-04-04 16:34

本文选题：益美肤　切入点：放射性皮肤损伤　出处：《南华大学》2015年硕士论文

【摘要】：目的:通过比较创面大体变化、组织学变化及表皮干细胞标记物K19及整合素β1阳性细胞表达方面的变化,探讨益美肤对SD大鼠皮肤急性β射线Ⅱ度皮肤损伤创面的修复的效果。方法:2-3月龄清洁级SD.大鼠102只,体重190±10g,单笼喂养5天以适应环境。实验动物创面制备时使用SIMENS直线加速器,参数设置6Me V高能电子束,单次照射大鼠臀部裸露皮肤11分钟52秒,制作SD大鼠急性β射线Ⅱ度皮肤损伤创面。将实验大鼠随机分.为空白组、对照组和实验组。实验组创面外.用.益美肤,每日三次;对照组创面外用比亚芬,每日三次;空白组创面不予处理。于放射后第1、3、5、7、14、21、35天,观察各组大鼠放射部位皮肤创面损伤及愈合情况后取放射部位皮肤组织,石蜡包埋切片后,一部分用于做HE染色用于组织病理学检查,另一部分做免疫组织化学检测(SP法)。使用Ipp6.0图像软件检测不同时相点创面表皮干细胞标记物K19和整合素β1阳性细胞表达情况。所有数据用SPSS18.0统计软件进行单因素方差分析,以p0.05为有统计学意义。结果:1、大体观察:从照射后的第3天开始,空白组照射区皮肤出现干性脱屑等反应,甚至出现局部充血性红斑。对.照组及实验组在照射后第5天开始出现红斑反应,但无明显脱屑,红斑颜色较浅;随着时间推移,空白组创面颜色加深明显并形成苔藓样坏死溃疡痂皮,创面脱痂愈合延迟,愈合过程中逐步变厚变硬呈挛缩状态,表面不平整,色泽较正常皮肤深,充血水肿明显,毛发生长稀疏。对照组.实验组愈合过程类似,被覆新生上皮,愈合部位较平整,颜色与正常皮肤相仿,毛发生长相对较多。2、创面组织学观察:与空白组比较,对照组与实验组的毛细血管肿胀程度较轻,到14天后新生毛细血管逐步增多,上皮细胞增生较活跃,炎症反应较轻。3、免疫组化结果:K19及integrinβ1在空白组、对照组、实验组均有阳性表达,在基底细胞层及毛囊周围可见大量棕黄色颗粒,其中实验组阳性细胞数量最多。空.白.组.在.同.一.时.相.点.的.K19、β1整.合.素IOD.值.分.别与对照组、实.验.组.比较差异有统计学意义(p0.05),对照组与实验组比较差异无统计学意义(p0.05)。各组K19、integrinβ1的IOD值均在第21天达峰值,尤以实验组峰值最高,随后表达逐步降低。结论:SD大鼠急性β射线Ⅱ度皮肤损伤创面外用益美肤后,表皮干细胞标记物integrinβ1及K19表达显著上调,增殖较活跃,加速创面上皮化进程,明显缩短创面愈合时间。益美肤对治疗大鼠急性β射线Ⅱ度皮肤损伤创面具有确切的疗效,疗效与比亚芬相当,具有临床使用和推广价值。
[Abstract]:Objective: to investigate the effect of Yimei skin on repairing the wounds of SD rats with acute 尾 -ray injury by comparing the gross changes, histological changes and the expression of K19 and integrin 尾 _ 1 positive cells in epidermal stem cells.Methods: two to three months old clean grade SDS.102 rats, weighing 190 卤10 g, were fed alone for 5 days to adapt to the environment.SIMENS linear accelerator was used in the preparation of experimental animal wounds. 6Me V high energy electron beam was set up and exposed skin on the buttocks of SD rats was irradiated for 11 minutes for 52 seconds. The wounds of acute 尾 -ray second-degree skin injury were made in SD rats.The experimental rats were randomly divided.Blank group, control group and experimental group.The experimental group was outside the wound.With.The wounds in the control group were treated with Biafen three times a day, and the wounds in the blank group were not treated.On the 35th day after irradiation, the skin tissue was taken from the radiation site of the rats after the injury and healing of the skin wound. After paraffin embedded sections, some of them were used for HE staining for histopathological examination.The other part was detected by immunohistochemical method.Ipp6.0 image software was used to detect the expression of K19 and integrin 尾 1 positive cells.All the data were analyzed by single factor ANOVA with SPSS18.0 software.Results 1. Gross observation: from the 3rd day after irradiation, dry desquamation appeared in the skin of the blank group, and even local congestive erythema appeared.Yes.The erythema reaction began to appear in the irradiation group and the experimental group on the 5th day after irradiation, but there was no obvious desquamation, and the erythema color was lighter. With the passage of time, the wound color of the blank group deepened obviously and formed the mossy necrotic ulcer callus, and the wound descab healing was delayed.During the healing process, thickening and hardening showed contracture, uneven surface, deeper color than normal skin, obvious hyperemia and edema, and sparse hair growth.Control group.The healing process of the experimental group was similar, the healing site was flat, the color was similar to the normal skin, and the hair growth was relatively more .2.The wound histological observation: compared with the blank group, the healing process of the experimental group was similar to that of the control group.The capillary swelling degree of the control group and the experimental group was mild. After 14 days, the neovascularization gradually increased, the epithelial cells proliferated more actively, and the inflammatory reaction was lighter. The immunohistochemical results showed that: K19 and integrin 尾 1 were in the blank group, and in the control group.A large number of brown granules were found in the basal cell layer and around the hair follicle, among which the number of positive cells in the experimental group was the most.Empty.White.Group.In.The same.I.When.PhasePoint.K19, 尾 1 integral.Together.Simple IOD.Value.Minutes.Do not and control group, solid.Test.Group.The difference was statistically significant (P 0.05), but there was no significant difference between the control group and the experimental group (P 0.05).The IOD value of K19 integrin 尾 1 reached its peak on the 21st day, especially in the experimental group, and then decreased gradually.Conclusion the expression of integrin 尾 1 and K19, the marker of epidermal stem cells, is up-regulated, the proliferation is more active, the process of wound epithelization is accelerated, and the wound healing time is shortened obviously after topical application of the skin in the rats with acute 尾 -ray skin injury.Yimeifu has a definite curative effect on acute 尾 -ray second-degree skin injury wound in rats, and the curative effect is equivalent to that of Biafen, which has clinical application and popularization value.
【学位授予单位】：南华大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R818

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