离心跑台运动对小鼠骨骼肌炎性因子、肌再生因子及血管再生因子表达的影响

发布时间：2018-04-08 17:51

  本文选题：离心跑台运动　切入点：骨骼肌　出处：《中国康复医学杂志》2017年11期

【摘要】：目的:观察小鼠离心运动后骨骼肌炎性因子、肌再生因子以及血管再生因子的表达变化,探讨其在离心运动骨骼肌损伤修复中可能发挥的作用。方法:32只雄性C57BL/6小鼠随机分为对照组(C组,n=8)和离心运动组(D组,n=24)。根据取材时间,离心运动组又分为3个小组(运动后1d,3d和7d组)。离心运动后不同时间点取双侧腓肠肌。HE染色观察骨骼肌形态学变化,比色法检测血浆肌酸激酶(CK)和乳酸脱氢酶(LDH)活性,荧光定量PCR检测炎性因子、肌再生因子及血管再生因子基因表达的变化。结果:小鼠下坡跑后骨骼肌纤维结构无明显破坏,无显著炎性细胞浸润。与对照相比,血浆CK和LDH活性在运动后各时间点均无显著变化(P0.05)。从基因层面看,炎性细胞标志物(MPO—嗜中性粒细胞标志物;F4/80—巨噬细胞标志物)在下坡跑后显著上升。部分炎症因子(如肿瘤坏死因子-α、白介素-1β、白介素-6)在下坡跑后显著上调。而肌再生调节因子中,只有机械生长因子和白血病抑制因子在运动后出现显著上调。而肌再生关键细胞——肌卫星细胞的特异性标记分子(分子标志—Pax7,增殖标记—Myo D,分化标记—myogenin)在离心运动后未出现显著变化(P0.05)。此外,其他与肌肉和血管再生密切相关的再生因子(如:胰岛素样生长因子1、肝细胞生长因子、尿激酶型纤溶酶原激活物、环氧合酶2、低氧诱导因子-1α、血管内皮生长因子、血管生成素1等)在离心运动后均未见显著变化(P0.05)。结论:从形态学表现、血清指标和基因表达综合来看,离心跑台运动并非小鼠骨骼肌损伤建模的理想方式。
[Abstract]:Aim: to observe the expression of inflammatory factor, muscle regeneration factor and vascular regeneration factor in skeletal muscle after eccentric exercise in mice, and to explore the possible role of these factors in the repair of skeletal muscle injury after eccentric exercise.Methods 32 male C57BL/6 mice were randomly divided into control group (C group) and centrifugal exercise group (group D).According to the sampling time, the centrifuge exercise group was divided into 3 groups (1 d after exercise on 3 d and 7 d after exercise).The morphological changes of skeletal muscle were observed by HE staining at different time points after centrifugation. The activities of creatine kinase (CK) and lactate dehydrogenase (LDH) in plasma were detected by colorimetry, and inflammatory factors were detected by fluorescence quantitative PCR.Changes of gene expression of muscle regeneration factor and vascular regeneration factor.Results: there was no obvious destruction of skeletal muscle fiber structure and no significant inflammatory cell infiltration after running downhill in mice.Compared with the control, the activities of CK and LDH in plasma did not change significantly at each time point after exercise.At the gene level, the inflammatory cell marker, MPO- neutrophil marker, F4 / 80-macrophage marker, increased significantly after downhill running.Some inflammatory factors (such as TNF-伪, IL-1 尾, IL-6) were significantly up-regulated after downhill running.Among the regulating factors of muscle regeneration, only mechanical growth factor and leukemia inhibitory factor were significantly up-regulated after exercise.However, the specific labeling molecules (molecular marker -Pax7, proliferative marker -Myo D, differentiation marker -myogenin) of muscle regeneration key cell-mysatellite cells did not show significant changes after centrifugation.In addition, other regeneration factors closely related to muscle and vascular regeneration (such as insulin-like growth factor 1, hepatocyte growth factor, urokinase-type plasminogen activator, cyclooxygenase-2, hypoxia inducible factor -1 伪, vascular endothelial growth factor),Angiopoietin-1, et al.) there was no significant change in P0.05 after centrifugal exercise.Conclusion: centrifugal treadmill exercise is not an ideal way to model skeletal muscle injury in mice from morphological manifestation, serum index and gene expression.
【作者单位】： 上海体育学院运动科学学院;井冈山大学体育学院;
【基金】：国家自然科学基金资助项目(31300975;31271273) 教育部博士点基金项目(20133156120004) 上海市人类运动能力开发与保障重点实验室(上海体育学院)资助项目(11DZ2261100)
【分类号】：R873
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本文编号：1722684