山菠菜多糖对长时间大强度运动大鼠铁代谢的影响及分子机制研究

发布时间：2018-04-15 09:31

本文选题：山菠菜多糖 + 铁代谢　；参考：《沈阳体育学院学报》2016年05期

【摘要】：为了探讨补充山菠菜多糖对长时间大强度运动大鼠铁代谢的影响及可能的分子机制,选取30只SD大鼠分为对照组(C组)、运动组(E组)和运动+干预组(EI组),利用跑台运动构建长时间大强度运动大鼠模型。从跑台运动第3周起,大鼠用100 mg/kg的山菠菜提取物悬浊液每天灌服2 ml,灌服3周后测定其血清Hepcidin、TF和铁状态,实时荧光定量PCR技术检测肝脏组织中TGF-β1、BMP-2和Smad4基因表达,Western blot法检测肝脏组织中IL蛋白和JAK/STAT信号通路蛋白表达。结果:与E组相比,补充山菠菜多糖能够有效降低血清Hepcidin和TF,改善机体铁缺乏状态,差异有统计学意义(P0.05),补充山菠菜多糖可有效抑制TGF-β1/BMP/Smad信号通路,也可通过降低肝脏组织中IL-6蛋白表达,而抑制JAK/STAT信号通路蛋白活性,差异有统计学意义(P0.05)。结论:补充山菠菜多糖可通过抑制TGF-β1/BMP/Smad信号通路及IL-6介导的JAK/STAT信号通路而减少Hepcidin表达,改善长时间大强度运动大鼠铁缺乏状态。
[Abstract]:In order to investigate the effect of supplementation of spinach polysaccharide on iron metabolism and its possible molecular mechanism in rats with long time and high intensity exercise,Thirty Sprague-Dawley rats were divided into two groups: control group (C group), exercise group (group E) and exercise intervention group (group I).From the third week of treadmill exercise, rats were given 2 ml of spinach extract suspension (100 mg/kg) daily. After 3 weeks of administration, the serum levels of hepcidinine TF and iron were measured.The expression of BMP-2 and Smad4 in liver tissue was detected by real-time fluorescence quantitative PCR. The expression of IL protein and JAK/STAT signal pathway protein in liver tissue was detected by Western blot.Results: compared with group E, supplementation of mountain spinach polysaccharides could effectively reduce serum Hepcidin and TFFs and improve iron deficiency status. The difference was statistically significant (P 0.05). Supplementation of mountain spinach polysaccharides could effectively inhibit TGF- 尾 1/BMP/Smad signaling pathway.The activity of JAK/STAT signaling pathway protein could be inhibited by decreasing the expression of IL-6 protein in liver tissue, and the difference was statistically significant (P 0.05).Conclusion: supplementation of spinach polysaccharides can reduce the expression of Hepcidin by inhibiting TGF- 尾 1/BMP/Smad signaling pathway and JAK/STAT signaling pathway mediated by IL-6, and ameliorate iron deficiency in rats with long time and high intensity exercise.
【作者单位】：吉林大学体育学院;
【基金】：吉林省发改委产业技术研究与开发项目(2016C055-3)
【分类号】：R87

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