茄尼醇对大鼠运动疲劳恢复期心肌自由基代谢和血清相关指标的影响

发布时间：2018-04-17 03:22

本文选题：茄尼醇 + 大鼠　；参考：《西北师范大学》2012年硕士论文

【摘要】：大强度运动过程中，机体内自由基生成量增加，自身的抗氧化系统难以清除过剩的自由基，是运动损伤和疲劳发生的重要原因。外源性抗氧化剂的补充可延缓或逆转由于氧化应激引起的各种慢性病理过程及运动性疲劳的发生和发展，提高运动尤其是有氧运动能力。茄尼醇是一种不饱和的聚异戊二烯醇，属四倍半萜醇。茄尼醇本身具有9个异戊二烯基团和多个非共轭双键，因此具有非常强烈的吸收自由基的性能。茄尼醇可以作为异戊二烯单元用于合成具有生物活性的泛醌类物质，如辅酶Q_(10)和维生素K_2类。而辅酶Q_(10)是一种代谢激活剂，可以改变细胞和组织的缺氧状态，同时，辅酶Q_(10)还是细胞自身产生的天然抗氧化剂，能抑制线粒体的过氧化，保护生物膜结构的完整性。实验目的：在运动训练中，运动性疲劳的产生、积累和消除是一个连续过程。本文通过大鼠运动实验，研究在运动与恢复期间疲劳的产生、积累和清除这一渐进的、连续的过程中，补充茄尼醇对递增运动负荷训练至力竭的运动性疲劳大鼠心肌和血清相关指标影响及其规律，旨在为探讨补充茄尼醇对延缓运动性疲劳的产生和加速疲劳的消除以及对大鼠心肌细胞的保护作用提供实验依据。实验方法：实验以健康雄性SD大鼠为研究对象，以递增负荷跑台训练方式建立运动性疲劳模型。将跑台适应训练、筛选后大鼠随机分为：安静组（UT）；运动即刻组(TC1)、恢复6h组(TC2)和恢复24h组(TC3)；运动小剂量即刻组(TST1)、恢复6h组(TST2)和恢复24h组(TST3)；运动大剂量即刻组(TLT1)、恢复6h组(TLT2)和恢复24h组(TLT3)。剂量组每天上午以按照药剂量3mg/100g（小剂量）、6mg/100g(大剂量)给予灌胃，下午按照运动疲劳方案进行训练，除安静组外，其余各组按递增负荷训练2周，中间不休息，2周训练后第1天，大鼠除安静组外，所有其他组最后一次全部以30m/min的速度一次性力竭。大鼠力竭后，按照不同恢复时间，摘除眼球采血，然后断髓处死，同时迅速取出心脏，分别检测大鼠心肌组织SOD、GSH-Px、MDA、CAT、SDH、LDH和血清的BUN和CK。实验结果：（1）运动力竭即刻，TST心肌组织SOD、GSH-PX、CAT、LDH、SDH的活性和SOD/MDA比值显著性（P0.01）高于TC，TLT也有所升高，与TC和TST相比，无显著性差异；TST心肌组织MDA活性显著性（P0.01）低于TC和TLT，TST血清CK活性最低，与TC和TLT无显著性差异，TST血清BUN显著性（P0.05）低于TC，与TLT无显著性差异。（2）运动力竭恢复6h, TST心肌组织GSH-PX、LDH、SDH的活性和SOD/MDA比值显著性（P0.01）高于TC，且显著性（P0.05）高于TLT；TST血清CK、BUN活性显著性（P0.05）低于TC，且TST血清BUN显著性（P0.05）低于TLT。（3）运动力竭恢复24h, TST心肌组织GSH-PX、CAT、LDH、SDH的活性和SOD/MDA比值显著性（P0.01）高于TC，其中，TST心肌组织CAT、LDH、的活性和SOD/MDA比值显著性（P0.01）高于TLT；TST血清CK、BUN活性显著性（P0.01）低于TC，且TST血清BUN显著性（P0.05）低于TLT。实验结论：（1）外源性补充外源性茄尼醇可不同程度提高运动大鼠恢复期心肌SOD/MDA比值GSH-PX和CAT活性。说明茄尼醇具有良好的抗脂质过氧化作用，可以降低由大量自由基引起的心肌缺血再灌注损伤程度和心肌细胞凋亡率，保护心肌细胞膜结构和功能完整性,促进运动疲劳的恢复。（2）茄尼醇可不同程度提高运动大鼠恢复期心肌LDH、SDH的活性，降低血清CK活性和尿素氮的含量，，从而缓解疲劳产生、增强运动能力；有利于有氧代谢过程，促进运动时心肌能量的供给和疲劳恢复。（3）小剂量对大鼠运动疲劳恢复的影响优于大剂量。
[Abstract]:Coenzyme Q _ ( 10 ) is a kind of unsaturated polyisoprenoid , which can delay or reverse the occurrence and development of various chronic diseases , such as coenzyme Q _ ( 10 ) and vitamin K _ 2 , which are caused by oxidative stress .

The aim of this study is to study the effects of solanesol on cardiac and serum - related indexes of exercise - induced fatigue in rats during exercise and recovery , and to provide experimental basis for the study of the effects of solanesol on the delayed exercise fatigue and the elimination of accelerated fatigue .

The experimental methods were as follows : the healthy male SD rats were used as the research objects , and the sports fatigue model was established by increasing the training mode of the load running platform . The treadmill was adapted to the training , and the rats after screening were randomly divided into : quiet group ( UT ) ;
exercise immediate group ( tcl ) , recover 6h group ( tc2 ) and recover 24h group ( tc3 ) ;
Immediate group ( TST1 ) , 6h group ( TST2 ) and 24h group were recovered ( Tst3 ) .
The rats were divided into three groups according to the daily dose of 3 mg / 100g ( small dose ) , 6 mg / 100g ( high dose ) and 6 mg / 100g ( high dose ) . The rats were divided into three groups according to the daily dose of 3 mg / 100g ( small dose ) and 6 mg / 100g ( high dose ) .

Experimental results :

( 1 ) Immediately after exercise , the activity of SOD , GSH - PX , CAT , LDH , SDH and SOD / MDA in myocardium were significantly higher than those of TC and TLT ( P0.01 ) .
There was no significant difference in serum CK activity between TLT and TLT ( P0.01 ) . There was no significant difference with TC and TLT . There was no significant difference in serum BUN ( P0.05 ) between TLT and TLT .

( 2 ) The activity of GSH - PX , LDH , SDH and the ratio of SOD / MDA were significantly higher than that of TC ( P0.01 ) .
There was significant difference ( P0.05 ) between serum CK and BUN ( P0.05 ) , and the serum BUN was lower than that of TLT ( P0.05 ) .

( 3 ) The activity of GSH - PX , CAT , LDH , SDH and the ratio of SOD / MDA ( P0.01 ) were higher than that of TC , and the activities of CAT , LDH , LDH , LDH , SDH and SOD / MDA were significantly higher than that of TLT ( P0.01 ) .
There was significant difference ( P0.01 ) between serum CK and BUN ( P0.01 ) , and the serum BUN was lower than that of TLT ( P0.05 ) .

Experimental Conclusion :

( 1 ) The activities of SOD / MDA and GSH - PX and CAT in the myocardium of rats with different degrees could be increased in different degrees . The results showed that solanesol had a good anti - lipid peroxidation effect , and could reduce the degree of myocardial ischemia reperfusion injury induced by a large number of free radicals and the apoptosis rate of myocardial cells , protect the membrane structure and functional integrity of myocardial cells , and promote the recovery of exercise fatigue .

( 2 ) solanesol can improve the activity of LDH and SDH in the recovery period of exercise rats in different degrees , reduce the serum CK activity and the content of urea nitrogen , thereby relieving fatigue and enhancing athletic ability ;
is beneficial to the aerobic metabolism process and promotes the supply and fatigue recovery of myocardial energy during exercise .

( 3 ) The effect of small dose on the recovery of exercise fatigue in rats was superior to that of large dose .

【学位授予单位】：西北师范大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：G804.2

【参考文献】