运动经PKC-Nrf2途径调节肝HO-1表达及抗氧化能力的研究

发布时间：2018-04-25 17:45

本文选题：耐力运动 + 肝脏　；参考：《广西师范大学学报(自然科学版)》2017年01期

【摘要】：为探讨规律耐力运动通过PKC-Nrf2途径提高大鼠肝血红素加氧酶-1(HO-1)表达及抗氧化能力(T-AOC)的效应,将24只成年雄性SD大鼠随机分为安静对照组(A)、运动组(B)与白屈菜赤碱运动组(C)。B、C组进行8周较大强度的持续耐力跑,但C组在每次运动前进行PKC抑制剂即白屈菜赤碱(CHE)处理。实验中观察各组肝细胞膜PKC及肝细胞总PKC的活性水平,Western Blotting观察肝细胞核Nrf2及肝细胞HO-1的蛋白表达水平,以及观察肝细胞HO-1活性和T-AOC水平,结果发现:与A组相比,B组肝细胞膜PKC及肝细胞总PKC的活性显著升高、细胞膜PKC活性/肝细胞总PKC活性的值显著升高(P0.05),细胞核中的Nrf2蛋白含量(核转位)显著增加(P0.05),肝细胞HO-1的蛋白表达水平、活性水平及肝细胞T-AOC等均显著升高(P0.05);耐力运动引起B组中的上述变化在C组中被明显遏制。综上可见,较大强度的规律耐力运动可经过PKC-Nrf2途径,诱导肝细胞HO-1蛋白表达而提高肝脏抗氧化能力。
[Abstract]:To investigate the effects of regular endurance exercise on the expression of heme oxygenase-1 (HO-1) and its antioxidant capacity (T-AOC) through PKC-Nrf2 pathway in rats. Twenty-four adult male Sprague-Dawley rats were randomly divided into two groups: control group (control group), exercise group (B group) and carrageenine group (group C) for 8 weeks of sustained endurance run, but group C was treated with PKC inhibitor (Che) before each exercise. The activity levels of PKC and total PKC of hepatocytes in each group were observed. The expression of Nrf2 and HO-1 protein, the activity of HO-1 and the level of T-AOC in hepatocytes were observed by Western Blotting. The results showed that compared with group A, the activities of PKC and total PKC of hepatocytes in group B were significantly higher than those in group A. The PKC activity of cell membrane / total PKC activity of hepatocytes increased significantly, while the Nrf2 protein content (nuclear translocation) in the nucleus increased significantly, and the expression level of HO-1 protein in the hepatocytes was also increased. The activity level and T-AOC of hepatocytes were significantly increased (P 0.05), and the above changes in group B induced by endurance exercise were significantly inhibited in group C. It can be concluded that high intensity regular endurance exercise can induce the expression of HO-1 protein through PKC-Nrf2 pathway and improve the antioxidant ability of liver.
【作者单位】：广西师范大学体育学院;
【基金】：广西自然科学基金(2013GXNSFBA019146)
【分类号】：R87

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