紫甘薯花青素对过度训练大鼠心肌氧化损伤干预的研究

发布时间：2018-04-26 02:33

本文选题：过度训练 + 花青素　；参考：《江苏师范大学》2013年硕士论文

【摘要】：研究目的：研究表明，自由基是过度训练对大鼠心肌氧化损伤的最直接物质，服用抗氧化性物质可以减少自由基的生成，降低心肌受到的氧化损伤。本研究以抗氧化物（紫甘薯花青素）对过度训练大鼠心肌氧化损伤的干预为切入点，分析抗氧化物（紫甘薯花青素）对过度训练大鼠心肌氧化损伤的影响，以寻求新的抗心肌氧化损伤的物质，丰富有关抗心肌氧化损伤的物质来源。本研究旨在探讨紫甘薯花青素的抗氧化作用对过度训练大鼠心肌氧化损伤的干预效果，为进一步研究紫甘薯花青素等抗氧化物对过度训练引起的心肌损伤的干预提供一个可靠的依据。研究方法：本研究采用动物实验法，正式实验之前先进行预实验，以健康雄性SD大鼠为实验对象。预实验选取较成熟的过度训练方案，进行六周的造模，然后取大鼠腹主动脉血液制备血清，测量判断过度训练的主指标：血睾酮（T）和皮质醇（C），通过分析指标,得出预实验中所用的训练方案能够成功的复制过度训练模型，该方案可以应用于正式实验当中。正式实验选取健康雄性SD大鼠84只，随机分为4大组，分别为空白对照组（C组）、一般训练组（NS组）、过度训练组（OT组）和过度训练花青素组（OTH组），其中所有运动组大鼠分两种处死状态，即安静状态下处死（后加1表示）和即刻状态下处死（后加2表示）。运动组大鼠周日至周五运动，周六休息，共进行6周，然后取大鼠腹主动脉血液制备血清并摘取心脏，零下80℃保存。实验结束后，制作心肌组织切片并进行HE染色病理学观察，测量分析血清指标心肌肌钙蛋白I（cTnI）、肌酸激酶同工酶（CK-MB）、乳酸脱氢酶（LDH）、肌酸激酶（CK）、血睾酮（T）和皮质醇(C)，大鼠心肌线粒体指标丙二醛(MDA)、超氧化物歧化酶(SOD)、线粒体内Ca2+浓度(Ca2+)，对检测结果进行相关统计学分析。研究结果： 1NS组超氧化物歧化酶、丙二醛、Ca2+浓度、血睾酮、皮质醇、乳酸脱氢酶、肌酸激酶、肌酸激酶同工酶、心肌肌钙蛋白I与C组对比均没有差异性；NS组与C组相比，肌纤维增粗，排列整齐有序。 2OT组超氧化物歧化酶、血睾酮与C组和NS组相比均显著降低,丙二醛、Ca2+浓度、皮质醇、乳酸脱氢酶、肌酸激酶、肌酸激酶同工酶、心肌肌钙蛋白I的水平均显著高于C组和NS组；组织切片表明OT组与C组和NS组相比，心肌过度肥大，肌纤维排列疏松错乱，有病态现象。 3OTH组超氧化物歧化酶、血睾酮水平显著低于C组和NS组；丙二醛、皮质醇、Ca2+浓度、乳酸脱氢酶、肌酸激酶、肌酸激酶同工酶、心肌肌钙蛋白I的水平均显著高于C组和NS组；OTH组超氧化物歧化酶、血睾酮水平高于OT组，但不显著；丙二醛、Ca2+浓度、皮质醇、乳酸脱氢酶、肌酸激酶、肌酸激酶同工酶、心肌肌钙蛋白I的水平低于OT组，其中心肌肌钙蛋白I、Ca2+浓度差异非常显著，其他指标显著。OTH组的组织切片也表现出了一定的病态现象，但是要轻于OT组。研究结论： 1适宜的运动训练能一定程度的提高心肌的抗氧化能力，能使心肌肌纤维增粗，排列更致密有序，引导心肌向着健康的方向发展，对心肌具有一定的保护作用。 2过度训练破坏了大鼠心肌内自由基的防御系统，心肌细胞结构被破坏，肌纤维过度肥大，排列错乱无规则，心肌组织受到一定程度的氧化损伤，有病态现象。 3紫甘薯花青素的干预能一定程度的提高过度训练大鼠的抗氧化能力，保护心肌细胞的结构，减轻肌纤维的排列错乱现象，病态现象减轻，降低了过度训练所引起的心肌氧化损伤程度。 4经过24小时的休息，一般训练大鼠心肌的轻微损伤能得到有效的修复；过度训练运动大鼠也有所修复，但是修复程度较小；紫甘薯花青素的干预可以在一定程度上加快过度训练大鼠心肌氧化损伤的修复速度。
[Abstract]:The purpose of the study is:
Studies have shown that free radicals are the most direct substances of oxidative damage to rats' Myocardium by overtraining. Taking antioxidant substances can reduce the formation of free radicals and reduce oxidative damage to the myocardium. The effect of sweet potato anthocyanins on oxidative damage of myocardium in overtrained rats, in order to seek new substances against myocardial oxidative damage, and to enrich the material sources of anti myocardial oxidative damage. The aim of this study was to explore the effect of antioxidant effects of purple sweet potato anthocyanins on myocardial oxygen damage in overtrained rats and to further study purple sweet potato Anthocyanins and other antioxidants provide a reliable basis for the intervention of myocardial injury induced by overtraining.
Research methods:
In this study, the animal experiment method was used. Before the formal experiment, a healthy male SD rat was taken as the experimental object. A more mature overtraining program was selected to make a model for six weeks. Then the blood serum was prepared from the abdominal aorta of rats. The main index of overtraining was measured: blood testosterone (T) and cortisol (C). It can be used in the formal experiment. 84 healthy male SD rats were randomly divided into 4 groups: blank control group (group C), general training group (group NS), overtraining group (OT group) and overtraining anthocyanin group. (group OTH), all of the rats in the exercise group were killed in two kinds of death States, that is, to be executed in a quiet state (after adding 1) and to be executed under immediate condition. The rats in the exercise group were exercise on Sunday to Friday, and the Saturday rest, for a total of 6 weeks. Then the rat abdominal aorta was taken to prepare the serum and extract the heart and save at minus 80. After the experiment, the experiment was made. Myocardial tissue sections and HE staining pathological observation were performed to measure and analyze serum cardiac troponin I (cTnI), creatine kinase isoenzyme (CK-MB), lactate dehydrogenase (LDH), creatine kinase (CK), serum testosterone (T) and cortisol (C), myocardial mitochondrial index of malondialdehyde (MDA), superoxide dismutase (SOD), and Ca2+ concentration in mitochondria (Ca2+). Statistical analysis was carried out on the results of the test.
The results of the study:
1NS group superoxide dismutase, malondialdehyde, Ca2+ concentration, blood testosterone, cortisol, lactate dehydrogenase, creatine kinase, creatine kinase isoenzyme, cardiac troponin I and C group were no difference, NS group and C group, muscle fiber thickened, arranged orderly and orderly.
The level of MDA, Ca2+, cortisol, creatine kinase, creatine kinase isoenzyme and cardiac troponin I were significantly higher in group 2OT than in group C and NS. The tissue section showed that OT group was hyperhypertrophy of myocardium and loosely arranged muscle fiber in group OT and C group and NS group. A disorder, a pathological phenomenon.
3OTH group superoxide dismutase, blood testosterone level was significantly lower than group C and NS, malondialdehyde, cortisol, Ca2+ concentration, lactate dehydrogenase, creatine kinase, creatine kinase isoenzyme, myocardial troponin I level were significantly higher than the C and NS groups; OTH group superoxide dismutase, blood testosterone level was higher than the OT group, but not significant; malondialdehyde, Ca2+ concentration Degree, cortisol, lactate dehydrogenase, creatine kinase, creatine kinase isoenzyme, cardiac troponin I level were lower than that of group OT, and the difference of cardiac troponin I and Ca2+ concentration was very significant. Other indicators also showed a certain pathological phenomenon in group.OTH, but it should be lighter than group OT.
The conclusions are as follows:
1 suitable exercise training can improve the antioxidant capacity of the myocardium to a certain extent, make the muscle fiber thickening, arrange more compact and orderly, guide the heart to the direction of health and have a certain protective effect on the myocardium.
2 over training destroys the free radical defense system in the rat myocardium, the structure of the myocardial cells is destroyed, the muscle fiber hypertrophy, the disorder of the arrangement is irregular, the myocardial tissue is damaged by some degree of oxidative damage, and there is a pathological phenomenon.
The intervention of 3 purple sweet potato anthocyanins can improve the antioxidant capacity of overtraining rats to a certain extent, protect the structure of cardiac myocytes, reduce the disorder of muscle fiber arrangement, reduce the pathological phenomenon, and reduce the degree of myocardial oxidative damage caused by overtraining.
4 after 24 hours of rest, the mild injury of the rat's myocardium can be effectively repaired, and the overtraining exercise rats are also repaired, but the repair degree is small, and the intervention of Purple Sweet Potato Anthocyanin can accelerate the repair speed of myocardial oxidative damage in rats to a certain extent.

【学位授予单位】：江苏师范大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R873

【参考文献】