运用多模态影像技术进行非酒精性脂肪肝定量分析的可行性研究

发布时间：2018-04-27 18:19

本文选题：非酒精性脂肪肝 + 定量诊断　；参考：《安徽医科大学》2017年硕士论文

【摘要】：目的:探讨运用双能量能谱CT及3.0T磁共振IDEAL-IQ(磁共振成像定量非对称回波的最小二乘估算法迭代水脂分离序列,Magnetic Resonance Imaging iterative decomposition of water and fat with echo asymmetry and least-squares estimation quantitation sequence)技术进行非酒精性脂肪肝定量分析的可行性。方法:50只雄性SD(Sprague-Dawley)大鼠给予高脂饲料喂养,从第6周起,每隔两周随机取10只大鼠进行MRI和双能量能谱CT扫描。使用GE ADW(Advantage Workstation)4.6工作站测量MRI IDEAL-IQ图像肝脏的脂肪分数,将MRI IDEAL-IQ所测脂肪分数定义5%为A组,5%-10%为B组,10%为C组,根据MRI IDEAL-IQ分组使用双能量能谱CT工作站GSI软件分析对应0.625mm和1.25mm下不同基物质对(脂/水、水/脂)下所测脂含量,并获取与之对应的能谱曲线,以上述测量选定ROI层面为参考取相应区域大鼠肝脏做病理检查,行HE染色(苏木精-伊红染色,hematoxylin-eosinstaining)作为脂肪含量测定的金标准。使用SPSS16.0软件首先分析上述3组间大鼠能谱CT以及MRI所测肝脏脂肪含量与病理结果的相关性,其次分析能谱CT不同基物质对(脂/水、水/脂)及单能量CT值所测脂含量与MRI IDEAL-IQ所测脂肪肝脂质含量之间的相关性。结果:50只模型组大鼠有45只大鼠肝脏发生脂肪变,造模成功率达90%。各组间脂肪细胞计数、MRI IDEAL-IQ所测脂肪分数以及能谱CT所测数值之间差异有统计学意义(P0.05);各组大鼠能谱CT脂/水基物质对所测脂质含量与HE染色结果之间的相关性较高(r_(脂/水)1.25=0.886,r_(脂/水0.625)=0.884),水/脂基物质对所测脂质含量与HE染色结果之间呈负相关性(r水/脂1.25=-0.879,r水/脂0.625=-0.876);60Ke V单能量CT值与HE染色结果呈负相关性(r_(CT1.25)=-0.855,r_(CT0.625)=-0.851);MRI IDEAL-IQ技术所测大鼠非酒精性脂肪肝脂肪分数与HE染色结果之间具有密切的相关性(r_(MRI)=0.975),能谱CT不同基物质对(脂/水、水/脂)所测脂含量与MRI IDEAL-IQ所测脂肪肝脂质含量之间的相关性为r’_(脂/水1.25)=0.906,r’_(脂/水0.625)=0.908,r’_(水/脂1.25)=-0.903,r’_(水/脂0.625)=-0.905;不同层厚能谱CT 60Ke V下CT值与MRI IDEAL-IQ所测脂肪肝脂质含量之间的相关性为r_(CT1.25)=-0.872,r_(CT0.625)=-0.870。脂肪含量越高,能谱衰减曲线弓背弧度越大。结论:雄性SD大鼠经高脂饲料喂养一段时间后可形成非酒精性脂肪肝。能谱CT与MRI IDEAL-IQ均可无创、准确的定量测量非酒精性脂肪肝的脂肪含量,其中,MRI IDEAL-IQ所测脂肪分数准确性较高。能谱CT脂、水基物质对所测脂含量、单能量CT值与MRI IDEAL-IQ所测脂肪分数也有一定的相关性。
[Abstract]:Objective: to investigate the use of dual-energy spectroscopic CT and 3.0T magnetic resonance IDEAL-IQ (least square estimation of quantitative asymmetric echo of magnetic resonance imaging) to iterate the magnetic Resonance Imaging iterative decomposition of water and fat with echo asymmetry and least-squares estimation quantitation sequence) technique for the separation of water and lipid sequences. Feasibility of quantitative analysis of refined fatty liver. Methods Fifty male SD rats were fed with high fat diet. From the 6th week, 10 rats were randomly selected for MRI and dual energy spectrum CT scans every two weeks. GE ADW(Advantage Workstation)4.6 workstation was used to measure the liver fat fraction in MRI IDEAL-IQ images. The fat fraction measured by MRI IDEAL-IQ was defined as 5% in group A, 5-10% in group B, 10% in group B, and 10% in group C. According to MRI IDEAL-IQ grouping, the lipid content measured under different base material pairs (lipid / water, water / fat) under 0.625mm and 1.25mm was analyzed by using GSI software of dual-energy spectrum CT workstation, and the corresponding energy spectrum curve was obtained. The ROI level was selected as the reference for the pathological examination of the liver in the corresponding region of rats. Hematoxylin-eosin staining (hematoxylin-eosin staining) was used as the gold standard for the determination of fat content. SPSS16.0 software was used to analyze the correlation between the pathological results and the liver fat content measured by MRI in the above three groups, and then to analyze the relationship between the lipid / water ratio of the three groups, and the relationship between the liver fat content and the pathological results of the three groups of rats. The correlation between lipid content measured by water / fat and single energy CT value and fatty liver lipid content measured by MRI IDEAL-IQ. Results there were 45 rats with fatty degeneration in the liver of 50 rats in the model group, and the success rate of the model was 90%. The difference of fat fraction measured by IDEAL-IQ and EDS CT was statistically significant (P 0.05). The correlation between lipid content and HE staining results of lipid / water base substance in each group was higher than that in other groups. There was a negative correlation between the lipid content measured by water / water and the results of HE staining r / L 1.25- 0.879rwater / lipid 0.625- 0.87660KEV single energy CT value was negatively correlated with the results of HE staining in rats with the results of HE staining and the correlation between the single energy CT value and the results of HE staining was negative correlation between the content of lipids measured by water / lipids and the results of HE staining. There was a close correlation between fat fraction of non-alcoholic fatty liver and HE staining results. The correlation between the lipid content measured in water / fat and the lipid content in fatty liver measured by MRI IDEAL-IQ is as follows: rtih (lipid / water 1.25 / water 0.906) RSP (lipid / water 0.625 ~ 0.908) (water / lipid 1.25 ~ -0.903 ~ 0.903 ~ 0.903 ~ 0.905); CT value under different slice thickness CT 60Ke V and measured by MRI IDEAL-IQ The correlation of lipid content in fatty liver was as follows: CT1. 25 and CT0. 872; CT0. 625 and CT0. 870. The higher the fat content, the greater the arc of the curve of energy spectrum attenuation. Conclusion: Non-alcoholic fatty liver can be formed in male SD rats after feeding with high fat diet for a period of time. Energy dispersive CT and MRI IDEAL-IQ were both noninvasive and accurate quantitative measurement of fat content in non-alcoholic fatty liver, among which the accuracy of fat fraction measured by IDEAL-IQ was higher than that of non-alcoholic fatty liver. There was also a certain correlation between the fat fraction measured by MRI IDEAL-IQ and the content of fat measured by energy dispersive CT, water based substance and single energy CT.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R575.5;R816.5;R445.2

【相似文献】