封闭型空间细胞培养板研制与验证

发布时间：2018-04-28 14:29

本文选题：细胞培养板 + 流路控制挡板　；参考：《航天医学与医学工程》2017年02期

【摘要】：目的为适用于空间特殊实验环境,并在细胞培养实验中更高效地使用试剂、提高细胞培养用品的污染防护能力,提出了一种内部流动路径可控、具备防渗漏、防污染能力的全透明和全封闭细胞培养板。方法基于有限元仿真对培养板核心结构—流路控制挡板进行了优化,以优化后的培养板为核心部件搭建细胞培养回路,并开展了中长周期细胞培养试验。结果最优化的三坝-纵向排列细胞培养板,可有效控制其内部流体的流动路径,进出口流速1 m L/min时流体剪切力仅4.8×10~(-5)Pa,流动死区几乎为0,以该培养板为核心构建的细胞培养回路中培养的细胞可正常生长15 d。结论该细胞培养板,可使腔内新旧细胞培养试剂更换及残留气体排出更彻底,防渗漏防污染能力大大提升,试剂使用率提高,能满足空间实验常用典型细胞系的中长期培养需求,适合于我国目前及将来的空间医学生物学实验应用场合。
[Abstract]:Objective in order to be suitable for special space experimental environment, to use reagents more efficiently in cell culture experiment, and to improve the pollution protection ability of cell culture products, a kind of internal flow path is controlled and anti-leakage is proposed. Anti-fouling ability of fully transparent and fully enclosed cell culture plates. Methods based on the finite element simulation, the core structure of the culture plate, the flow control baffle, was optimized. The cell culture loop was set up with the optimized culture plate as the core component, and the medium and long period cell culture experiment was carried out. Results the optimized three-dam-longitudinal cell culture plate can effectively control the flow path of the internal fluid. At the flow rate of 1 m L/min, the flow shear stress was only 4.8 脳 10 ~ (-5) Pa, and the flow dead zone was almost zero. The cells cultured in the cell culture loop constructed with the culture plate as the core could grow normally for 15 days. Conclusion the new and old cell culture reagents can be replaced and the residual gas can be discharged more thoroughly, the ability of preventing leakage and pollution can be greatly improved, and the utilization rate of reagents can be increased by using the new and old cell culture plates. It can meet the medium and long term culture needs of typical cell lines in space experiments, and is suitable for the present and future applications of space medical biology experiments in China.
【作者单位】：中国航天员科研训练中心航天医学基础与应用国家重点实验室;
【基金】：航天医学基础与应用国家重点实验室资助项目(SMFA13A03,SMFA15A02)
【分类号】：R85

【相似文献】