耐力训练对帕金森模型小鼠中脑线粒体自噬相关基因表达的影响

发布时间：2018-05-02 00:18

  本文选题：耐力训练 + 帕金森病　； 参考：《中国康复医学杂志》2015年05期

【摘要】：目的:探讨耐力训练对帕金森病(PD)模型小鼠中脑线粒体自噬的影响,揭示运动预防帕金森病的分子生物学机制。方法:10周龄雄性C57BL/6小鼠32只,随机分为安静组(C),运动组(E),帕金森模型安静组(P)及帕金森模型运动组(PE),每组8只。E组及PE组小鼠进行为期8周跑台耐力训练,跑台速度15m/min,坡度为0%,每天运动持续50min,每周6次,周日休息。训练结束后,P组和PE组小鼠腹腔注射MPTP(30mg/kg),C组和E组小鼠注射等量生理盐水,持续3d。注射结束1周后,处死各组小鼠,取双侧中脑及纹状体,采用实时PCR及Western blot技术检测线粒体自噬相关基因及TH蛋白表达,HPLC技术检测中脑及纹状体组织内DA含量。结果:与C组比,E组小鼠中脑TH蛋白上调(P0.05),中脑及纹状体DA含量均增加(P0.05),P组和PE组小鼠TH蛋白及DA含量均显著下降(P0.01),而P组较PE组相应指标下降更明显(P0.05)。与C组比,E组小鼠中脑PINK、PARK2 m RNA及PINK1蛋白表达上调(P0.05),LC3Ⅱ/Ⅰ比值增加,P组小鼠PINK1m RNA表达下调(P0.05),PE组小鼠PINK1 m RNA、Parkin蛋白含量及LC3Ⅱ/Ⅰ比值较P组增加。结论:为期3d的MPTP处理可导致小鼠中脑及纹状体TH及DA含量下降,诱导帕金森病发生,耐力训练可缓解这一现象;MPTP诱导的帕金森小鼠模型中脑线粒体自噬水平下降,而耐力训练可作用于线粒体自噬相关基因的表达,抑制MPTP的神经毒性作用。
[Abstract]:Aim: to investigate the effects of endurance training on mitochondrial autophagy in midbrain of PD mice and to reveal the molecular biological mechanism of exercise in preventing Parkinson's disease. Methods Thirty-two male 10 week-old male C57BL/6 mice were randomly divided into three groups: control group, exercise group, Parkinson model group (P) and Parkinson's model exercise group (P). Each group of 8 mice in .E group and PE group were trained for 8 weeks. Running at a speed of 15 m / min, with a slope of 0, running 50 minutes a day, six times a week, and resting on Sundays. After training, mice in P group and PE group were intraperitoneally injected with 30 mg / kg MPTPN in group C and E, respectively. The same amount of normal saline was injected into mice of group E for 3 days. One week after the injection, the mice were killed and the contents of DA in the midbrain and striatum were detected by real-time PCR and Western blot. The expression of th protein and mitochondrial autophagy related gene were detected by HPLC. Results: compared with group C, th protein in mesencephalon of mice in E group was up-regulated, and DA content in midbrain and striatum were increased. Th protein and DA content in P0.05 group and PE group were significantly decreased compared with that in group C, while in group P, the decrease of corresponding indexes in group P was more obvious than that in group PE. Compared with group C, the expression of PINKT PARK2 m RNA and PINK1 protein in the midbrain of E group was increased compared with that in P group. The increase of PINK1m RNA expression in P group was higher than that in P group. Compared with that in P group, the expression of PINK1m RNA in P group decreased PINK1 m RNA-parkin protein content and the ratio of LC3 鈪，

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