自拟养阴生津方对辐射损伤小鼠HPRT基因防护作用研究

发布时间：2018-05-04 20:45

  本文选题：自拟养阴生津方 + 辐射损伤　； 参考：《南华大学》2012年硕士论文

【摘要】：目的： 探讨自拟养阴生津方对辐射损伤小鼠hprt基因防护作用，丰富中医药抗辐射理论，为中医药方剂预防放射辐射和降低放射辐射所导致的损害提供可检测的实验方法和理论指导。 方法： 动物分组：取雄性健康昆明种小鼠200只随机分为五组，每组40只。A.生理盐水组、B.照射1组、C.照射2组、D.中药1组、E.中药2组；照射：一次性给予B组、D组8Gy剂量的X射线。多次给予C组、E组总剂量为8Gy的X射线，1Gy/d连续照射8天。给药：给D.组、E.组小鼠用自拟养阴生津方灌胃，1次/d，0.5ml/次/只.其余各组小鼠用生理盐水以同样方法灌胃，连续10天，然后照射。照射期及其后继续给药，直至该组实验结束。检测及观察：照射结束后第5天，每组取10只小鼠处死，检测各组小鼠hprt基因突变率和突变谱。观察每组30只小鼠的30天存活率及平均存活天数。 结果： 1.小鼠的存活率及平均存活天数。各组小鼠30天存活率分别：A.生理盐水组100%，B.照射1组0.00%，C.照射2组13.3%，D.中药1组16.7%， E.中药2组26.7%。各组小鼠平均存活天数分别为：A.生理盐水组30.0±0.0天，B.照射1组8.3±1.3天，C.照射2组13.1±4.2天，D.中药1组12.1±3.3天， E.中药2组16.4±1.7天。照射1组、照射2组小鼠30天存活率及平均存活天数与生理盐水组比较均明显降低，差异有显著性(P0.01)；给予小鼠自拟养阴生津方后，中药1组存活率及平均存活天数与照射1组比较有明显提高，差异有显著性(P0.05)；中药2组存活率及平均存活天数与照射2组比较有明显提高，差异有显著性(P0.05)。 2.各组小鼠外周血淋巴细胞hprt基因的突变率分别为：A.生理盐水组0.35±0.02‰，B.照射1组0.80±0.07‰，C.照射2组0.98±0.02‰，D.中药1组0.72±0.02‰，E.中药2组0.85±0.01‰。照射1组、2组小鼠hprt基因的突变率与生理盐水组比较均明显升高，差异有显著性(P0.01)；给予小鼠自拟养阴生津方后，中药1组小鼠hprt基因的突变率与照射1组比较有明显降低，差异有显著性(P0.05)；中药2组小鼠hprt基因的突变率与照射2组比较有明显降低，差异有显著性(P0.05)。 3.各组小鼠外周血淋巴细胞hprt基因的突变谱缺失突变比例分别为：A.生理盐水组25％，B.照射1组75％， C.照射2组87.5％，， D.中药1组50％，E.中药2组62.5％。照射1组、2组小鼠hprt基因的突变谱缺失突变比例与生理盐水组比较均明显升高，差异有显著性(P0.01)；给予小鼠自拟养阴生津方后，中药1组小鼠hprt基因的突变谱缺失突变比例与照射1组比较有明显降低，差异有显著性(P0.05)；中药2组小鼠hprt基因的突变谱缺失突变比例与照射2组比较有明显降低，差异有显著性(P0.05)。 结论： 1.自拟养阴生津方能提高辐射小鼠的30天存活率及平均存活天数。 2.自拟养阴生津方能降低辐射小鼠hprt基因突变率。 3.自拟养阴生津方能降低辐射小鼠hprt基因突变谱缺失突变比例。
[Abstract]:Purpose :

This paper discusses the protective effect of self - prepared yin - generating body on hprf gene in radiation - injured mice , enriches the theory of anti - radiation of traditional Chinese medicine , and provides the experimental method and theoretical guidance for the prevention of radiation radiation and the reduction of radiation induced by Chinese medicinal prescription .

Method :

Animal grouping : 200 male healthy Kunming mice were randomly divided into five groups : 40 . A . physiological saline group , B . irradiation group 1 , C . irradiation group 2 , D . Chinese medicine 1 group , E . traditional Chinese medicine 2 group ;
Two groups of mice were treated with normal saline for 8 days . The total dose of group E was 8Gy , and the dose of 1Gy / d was given for 8 days . The results were as follows : 5 days after the end of irradiation , 10 mice were sacrificed and the mutation rate and mutation spectrum of the mice were detected . The survival rate and the average survival days of 30 days in each group were observed .

Results :

1 . The survival rate and the average survival days of mice in each group were 100 % and 100 % in group A , 0.00 % in group B , 13.3 % in group C , 16.7 % in group B , and 26.7 % in group E . The average survival days of mice in each group were as follows : A . Saline group 30.0 卤 0 . 0 days , D . Chinese medicine 1 group 12.1 卤 3.3 days , E . Chinese medicine group 2 group 16.4 卤 1.7 days . The survival rate and average survival days of irradiated group 2 mice were significantly lower than those in physiological saline group , and the difference was significant ( P0.01 ) .
The survival rate and the average survival time of the traditional Chinese medicine group were significantly increased compared with those in the irradiated group ( P0.05 ) .
The survival rate and average survival time of the traditional Chinese medicine group were significantly improved compared with those in group 2 ( P0.05 ) .

2 . The mutation rate of hpr1 gene in peripheral blood lymphocytes of each group was 0 . 35 卤 0 . 02 鈥

本文编号：1844584