运动训练和表没食子儿茶素没食子酸酯对2型糖尿病大鼠肾脏TLR4炎性因子表达的作用研究

发布时间：2018-05-06 20:55

本文选题：型糖尿病 + 运动训练　；参考：《西安体育学院学报》2016年06期

【摘要】：目的探讨运动训练、表没食子儿茶素没食子酸酯(EGCG)单独干预及联合应用对2型糖尿病大鼠肾脏血管通透性和肾TLR4、NF-κBp65、IL-1β蛋白表达量的影响。方法通过高脂喂养加腹腔注射小剂量链脲佐菌素(STZ)构建2型糖尿病SD大鼠模型。将造模成功的56只大鼠随机分为糖尿病对照组(D)、糖尿病给药组(DY)、糖尿病运动组(DE)、糖尿病运动给药组(DEY),每组14只,另设正常对照组(C)12只。运动组大鼠进行12周不负重游泳训练,每周训练5 d。EGCG给药组大鼠每周灌胃7 d。实验结束后检测大鼠FBG、尿蛋白、UAER、TLR4、NF-κBp65、IL-1β蛋白和肾脏血管通透性变化,并对实验结果进行统计分析。结果与正常对照组(C)相比,糖尿病对照组(D)的FBG、尿蛋白、UAER、TLR4、NF-κBp65、IL-1β蛋白和肾脏血管通透性均显著升高(P0.01);糖尿病给药组(DY)、糖尿病运动组(DE)、糖尿病运动给药组(DEY)的FBG、尿蛋白、UAER、TLR4、NF-κBp65、IL-1β蛋白和肾脏血管通透性较糖尿病对照组(D)均显著降低(P0.05或P0.01);糖尿病运动给药组(DEY)FBG、UAER、TLR4、NF-κBp65、IL-1β蛋白表达量和肾血管通透性均显著低于糖尿病给药组(DY)(P0.05或P0.01),糖尿病运动给药组(DEY)肾脏指数、尿蛋白、UAER、TLR4、NF-κBp65、IL-1β蛋白表达量和肾血管通透性均显著低于糖尿病运动组(DE)(P0.05)。结论 2型糖尿病大鼠存在肾组织炎性因子表达增多,运动训练和EGCG可改善2型糖尿病大鼠肾脏炎性损伤,抑制TLR4信号通路。
[Abstract]:Objective to investigate the effects of exercise training, epigallocatechin gallate (EGCGG) alone and combined therapy on renal vascular permeability and renal TLR4NF- 魏 Bp65 / IL-1 尾 protein expression in type 2 diabetic rats. Methods SD rat model of type 2 diabetes was established by high fat feeding and intraperitoneal injection of streptozotocin (STZ). Fifty-six rats were randomly divided into three groups: control group (n = 14), control group (n = 12), diabetic exercise group (n = 14), diabetic exercise group (n = 14) and diabetic exercise group (n = 14). The rats in the exercise group were trained for 12 weeks without weight bearing swimming, and the rats in the control group were given 5 d.EGCG weekly intragastric perfusion for 7 days. At the end of the experiment, the changes of FBG, urinary protein TLR4, NF- 魏 Bp65, IL-1 尾 and renal vascular permeability in rats were detected and the results were statistically analyzed. Results compared with the normal control group, FBG, urinary protein TLR4, NF- 魏 Bp65, IL-1 尾 and renal vascular permeability in diabetic control group were significantly higher than those in diabetic group, diabetic exercise group and diabetic exercise group. Urinary protein UAERTLR4NF-Bp65 尾 and renal vascular permeability were higher than those in diabetic group. The expression of IL-1 尾 protein and renal vascular permeability in diabetic exercise group were significantly lower than those in diabetic group (P 0.05 or P 0.01), and the renal index in diabetic exercise group was significantly lower than that in diabetic exercise group (P 0.05 or P 0.01), and the expression of IL-1 尾 protein in TLR4 and renal vascular permeability was significantly lower in diabetic exercise group than that in diabetic exercise group, and the expression of IL-1 尾 and renal vascular permeability were significantly lower in the control group than in the diabetic exercise group, and the renal vascular permeability was significantly lower in the diabetic exercise group than that in the diabetic exercise group. The expression of NF- 魏 Bp65- 尾 and renal vascular permeability in UAR4 were significantly lower than those in diabetic exercise group (P 0.05). Conclusion there is an increase in the expression of renal inflammatory factors in type 2 diabetic rats. Exercise training and EGCG can improve the renal inflammatory injury and inhibit the TLR4 signal pathway in type 2 diabetic rats.
【作者单位】：河南大学体育学院;苏州大学体育学院;
【基金】：河南省教育厅科学技术研究重点项目(14B890010,14B890009)
【分类号】：R87

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